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Research ArticleBIOENERGETICS AND PHOTOSYNTHESIS
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Comparative Proteomics of Chloroplast Envelopes from C3 and C4 Plants Reveals Specific Adaptations of the Plastid Envelope to C4 Photosynthesis and Candidate Proteins Required for Maintaining C4 Metabolite Fluxes

Andrea Bräutigam, Susanne Hoffmann-Benning, Andreas P.M. Weber
Andrea Bräutigam
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Susanne Hoffmann-Benning
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Andreas P.M. Weber
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Published September 2008. DOI: https://doi.org/10.1104/pp.108.121012

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    Figure 1.

    Schematic representation of central carbon metabolism and associated transport processes in C3 chloroplasts and C4 PCA-type chloroplasts. In C3 chloroplasts, for three carbons fixed, at most one transport process is required; in C4 PCA-type chloroplasts, for three carbons fixed, at least 12 transport processes are required.

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    Figure 2.

    The envelope proteomes are similar when analyzed qualitatively. The percentage of proteins within one proteome is plotted. They are similar with regard to their physicochemical properties (solubility in organic solvents and the presence of membrane attachment structures; A) and with regard to their predicted targeting and percentage of novel proteins (B).

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    Figure 3.

    Quantitatively, the envelope proteomes differ in selected proteins. The relative abundance in C3-type envelopes was subtracted from the relative abundance in C4 PCA-type envelopes, and the difference was plotted for each protein. The dotted lines represent differences of ±0.5%.

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    Figure 4.

    For the extremes and selected unchanged proteins from Figure 3, detailed results were plotted. A, Selected proteins that do not change significantly in relative abundance. B, The 12 proteins that are lowest in relative abundance in C4 PCA-type envelopes compared with C3-type envelopes. C, The 12 proteins that are highest in relative abundance in C4 PCA-type envelopes compared with C3-type envelopes.

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Comparative Proteomics of Chloroplast Envelopes from C3 and C4 Plants Reveals Specific Adaptations of the Plastid Envelope to C4 Photosynthesis and Candidate Proteins Required for Maintaining C4 Metabolite Fluxes
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Comparative Proteomics of Chloroplast Envelopes from C3 and C4 Plants Reveals Specific Adaptations of the Plastid Envelope to C4 Photosynthesis and Candidate Proteins Required for Maintaining C4 Metabolite Fluxes
Andrea Bräutigam, Susanne Hoffmann-Benning, Andreas P.M. Weber
Plant Physiology Sep 2008, 148 (1) 568-579; DOI: 10.1104/pp.108.121012

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Comparative Proteomics of Chloroplast Envelopes from C3 and C4 Plants Reveals Specific Adaptations of the Plastid Envelope to C4 Photosynthesis and Candidate Proteins Required for Maintaining C4 Metabolite Fluxes
Andrea Bräutigam, Susanne Hoffmann-Benning, Andreas P.M. Weber
Plant Physiology Sep 2008, 148 (1) 568-579; DOI: 10.1104/pp.108.121012
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Plant Physiology: 148 (1)
Plant Physiology
Vol. 148, Issue 1
September 2008
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  • Steady-State Phosphorylation of Light-Harvesting Complex II Proteins Preserves Photosystem I under Fluctuating White Light
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