Article Figures & Data
Figures
- Figure 1.
Whole cell absorption spectra of the wild type (WT), CB, CK, and PAL. Note that in the mutants, the absorbance in the 625-nm region is progressively decreased, indicating a decrease in PC. Each spectrum was normalized to the same value at an optical density at 750 nm and then offset by 0.2 absorbance units for clarity.
- Figure 2.
Thin-section electron micrographs of Synechocystis 6803 wild type and phycobilisome antenna mutants. A, Wild-type (WT) strain with intact phycobilisomes. B, CB mutant with one PC hexamer per rod. C, CK mutant that contains only the APC core. D, PAL mutant that lacks the assembled phycobilisomes. Labeled are thylakoid membranes (T), polyphosphate bodies (P), and carboxysomes (C). A cartoon model of the phycobilisome structure in each strain is shown as an inset. The APC core is represented by the central blue circle, and the PC discs are shown as teal ellipsoids. Bars = 250 nm. [See online article for color version of this figure.]
- Figure 3.
MCR models for the wild type and phycobilisome mutants. A, MCR-derived pure spectral components for CB, CK, and PAL. B, MCR model for the wild type and PAL, where PSII and PSI have been constrained and are spectrally identical to their counterparts in A. The data have been normalized to unit length. For details on how the models were determined, see “Materials and Methods.”
- Figure 4.
A, MCR-derived concentration maps for PC, APC, PBS, PSII, and PSI components within each cell type. Each image box is 10 × 10 μm. Note that the color scale was constructed from 0 to maximum fluorescence intensity and is unique for each image. A red-green composite image was constructed to highlight the spatial segregation between PSII (red) and PSI (green) and is displayed in the right-most panel of A. The fluorescence intensities presented here have been calculated at the 99.5% confidence interval (see “Materials and Methods”). B, Surface plot of the per-pixel PSII-PSI ratio for the representative cells boxed in the composite images in A. Note that the color scale in B is uniform for each cell type. WT, Wild type.
- Figure 5.
Statistical box plots of the mean intensities for PSI (A) and PSII (B) from all cells for each cell type. The red center line represents the median of these values, and the 95% confidence band is denoted by notches. The bottom and top of the box represent the 25th and 75th percentiles, respectively. The dotted extended lines indicate ±2.7σ (99.3% data coverage), and the red crosses represent statistical outliers. n = 332 cells. WT, Wild type. [See online article for color version of this figure.]
Additional Files
Supplemental Data
Supplemental Figures and Movies
Files in this Data Supplement:
- Supplemental Data - Supplemental Figures 1-3
- Supplemental Data - Supplemental Figure and Movie Legends
- Supplemental Data - Supplemental Movie 1
- Supplemental Data - Supplemental Movie 2
- Supplemental Data - Supplemental Movie 3
- Supplemental Data - Supplemental Movie 4
- Supplemental Data - Supplemental Movie 5
