Table of Contents
ON THE INSIDE
Editor’s Choice
Editor’s Choice: Evaluating the Potential for Adverse Interactions within Genetically Engineered Breeding StacksWhen genetically engineered stacks (also known as stacked or combined events) are produced by combining two or more single transgenic events by conventional breeding, the potential for interactions between the products of the events that impact food and feed safety can be evaluated and the need for further safety assessment can be determined on a case-by-case basis.
BREAKTHROUGH TECHNOLOGIES
Correlative Imaging of Fluorescent Proteins in Resin-Embedded Plant Material1A simple technique permits fluorescent proteins to be imaged in resin-embedded plant material.
Production of a High-Efficiency TILLING Population through PolyploidizationConversion of diploid Arabidopsis to autotetraploidy enables denser mutagenesis, resulting in a highly efficient population for reverse genetics.
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BIOCHEMISTRY AND METABOLISM
Overexpression of a BAHD Acyltransferase, OsAt10, Alters Rice Cell Wall Hydroxycinnamic Acid Content and SaccharificationAn acyltransferase reduces cross linking in grass cell walls, yielding grass leaves and stems that can be more easily broken down to make biofuels.
Reciprocal Control of Anaplerotic Phosphoenolpyruvate Carboxylase by in Vivo Monoubiquitination and Phosphorylation in Developing Proteoid Roots of Phosphate-Deficient Harsh HakeaA novel pattern of in vivo posttranslational modifications activates phosphoenolpyruvate carboxylase in maturing proteoid roots of harsh hakea.
The Regulatory Properties of Rubisco Activase Differ among Species and Affect Photosynthetic Induction during Light TransitionsThe regulatory properties of Rubisco activase from different species suggest a new strategy for enhancing photosynthetic performance by increasing the rate of photosynthetic induction.
Reprogramming the Phenylpropanoid Metabolism in Seeds of Oilseed Rape by Suppressing the Orthologs of REDUCED EPIDERMAL FLUORESCENCE1Metabolic redirection in seeds of oilseed rape defines the role of an aldehyde dehydrogenase as a branch-point enzyme in the plant phenylpropanoid pathway.
Understanding the Role of Defective Invertases in Plants: Tobacco Nin88 Fails to Degrade SucroseAn inactive invertase may indirectly stimulate the activity of active cell wall invertases.
Autophagy Contributes to Nighttime Energy Availability for Growth in ArabidopsisAutophagic recycling of proteins supplies nighttime energy sources such as amino acids under conditions of limited sugar availability.
Chromoplast-Specific Carotenoid-Associated Protein Appears to Be Important for Enhanced Accumulation of Carotenoids in hp1 Tomato FruitsA chromoplast protein may assist in the sequestration and stabilization of carotenoids.
CELL BIOLOGY
- Multiple Functions of Kip-Related Protein5 Connect Endoreduplication and Cell Elongation
The cell cycle inhibitor KRP5 binds chromatin to coordinately control endoreduplication and chromatin structure and to allow the expression of genes required for cell elongation.
Distribution of Transglutaminase in Pear Pollen Tubes in Relation to Cytoskeleton and Membrane DynamicsDistribution of a cell wall enzyme in pollen depends both on actin filaments and membrane trafficking.
The Rab GTPase RabG3b Positively Regulates Autophagy and Immunity-Associated Hypersensitive Cell Death in ArabidopsisA Rab GTPase protein connects autophagy with plant immunity-triggered hypersensitive response and programmed cell death.
Time-Resolved Fluorescence Imaging Reveals Differential Interactions of N-Glycan Processing Enzymes across the Golgi Stack in PlantaThe biophysical technique of in planta FRET-FLIM provides evidence for the existence of homo- and heteromeric N-glycan processing enzyme complexes, which are predominantly formed between cis- and medial-Golgi enzymes.
AtMMS21, an SMC5/6 Complex Subunit, Is Involved in Stem Cell Niche Maintenance and DNA Damage Responses in Arabidopsis RootsAtMMS21 acts in double-strand break amelioration and stem cell niche maintenance during Arabidopsis root development.
Traffic of Human α-Mannosidase in Plant Cells Suggests the Presence of a New Endoplasmic Reticulum-to-Vacuole Pathway without Involving the Golgi ComplexNoncanonical protein traffic from the endoplasmic reticulum to the vacuole may bypass the Golgi complex altogether.
Chloroplast DNA Replication Is Regulated by the Redox State Independently of Chloroplast Division in Chlamydomonas reinhardtiiChloroplast DNA replication is regulated by the redox state in the cell, which is sensed by the chloroplast nucleoids in Chlamydomonas reinhardtii.
ECOPHYSIOLOGY AND SUSTAINABILITY
Transcriptome Responses to Combinations of Stresses in ArabidopsisIn Arabidopsis, the response of the majority of the genes cannot be predicted from single stress experiments and only a small fraction of the genes have potential antagonistic responses, indicating that plants have evolved to cope with combinations of stresses and therefore may be bred to endure them.
LESION SIMULATING DISEASE1, ENHANCED DISEASE SUSCEPTIBILITY1, and PHYTOALEXIN DEFICIENT4 Conditionally Regulate Cellular Signaling Homeostasis, Photosynthesis, Water Use Efficiency, and Seed Yield in ArabidopsisGene functions should be studied not only under stable laboratory conditions, but also in the environment abounding in multiple stresses.
Introgression of Novel Traits from a Wild Wheat Relative Improves Drought Adaptation in WheatAgropyron elongatum introgression into bread wheat (Triticum aestivum) improves root traits for drought adaptation.
In Vivo Visualizations of Drought-Induced Embolism Spread in Vitis viniferaTime-lapse x-ray tomography uncovers the importance of intervessel connections in the xylem network in drought-induced embolism.
GENES, DEVELOPMENT, AND EVOLUTION
A Developmental Transcriptional Network for Maize Defines Coexpression ModulesAnalyzing transcript abundance between tissues and during development identifies sets of coexpressed genes and related transcriptional controls.
Prevalent Role of Gene Features in Determining Evolutionary Fates of Whole-Genome Duplication Duplicated Genes in Flowering PlantsSome gene features, including evolution rate and gene structural complexity, are highly correlated to a gene’s evolutionary fate after whole-genome duplication.
Oleosin of Subcellular Lipid Droplets Evolved in Green AlgaeOleosin, the surface structural protein on plant seed oil bodies, evolved in green algae in which the oleosin genes have weak but cell/development-specific expression.
Widespread Long Noncoding RNAs as Endogenous Target Mimics for MicroRNAs in PlantsA computational method systematically identifies intergenic or noncoding gene-originated target mimics (eTMs) for 20 conserved microRNAs in Arabidopsis and rice and validates the biological functions of some eTMs.
A Role for MORE AXILLARY GROWTH1 (MAX1) in Evolutionary Diversity in Strigolactone Signaling Upstream of MAX2Phylogenetic and functional analysis of strigolactone pathway genes across the plant kingdom suggests considerable promiscuity in events upstream allowing for signal diversity and its later refinement.
Spatiotemporal Seed Development Analysis Provides Insight into Primary Dormancy Induction and Evolution of the Lepidium DELAY OF GERMINATION1 GenesExpression of a set of related proteins in Arabidopsis and its relative Lepidium papillosum (Brassicaceae) precede the onset of seed dormancy.
Potential Functional Replacement of the Plastidic Acetyl-CoA Carboxylase Subunit (accD) Gene by Recent Transfers to the Nucleus in Some Angiosperm LineagesFunctional transfer of a chloroplast gene gene to the nucleus of Campanulaceae species is a remarkable example of the processes underpinning endosymbiotic evolution.
Jasmonate Controls Leaf Growth by Repressing Cell Proliferation and the Onset of Endoreduplication while Maintaining a Potential Stand-By ModeThe plant hormone jasmonate inhibits leaf growth by delaying the switch from the mitotic cell cycle to the endoreduplication cycle and maintains the cell in a stand-by mode but ready-to-go after the stress.
Early Induction of Apple Fruitlet Abscission Is Characterized by an Increase of Both Isoprene Emission and Abscisic Acid ContentIsoprene is an early marker of apple fruitlet abscission induction and its emission is correlated with the levels of bioactive abscisic acid and the activation of the abscission zone.
Rice LHS1/OsMADS1 Controls Floret Meristem Specification by Coordinated Regulation of Transcription Factors and Hormone Signaling PathwaysIntegration of transcriptional and signaling pathways promote rice floret meristem specification and organ development.
AGAMOUS-Like15 Promotes Somatic Embryogenesis in Arabidopsis and Soybean in Part by the Control of Ethylene Biosynthesis and ResponseThe MADS domain transcription factor AGL15 promotes somatic embryogenesis in part through the control of ethylene biosynthesis and response in Arabidopsis and soybean.
MEMBRANES, TRANSPORT AND BIOENERGETICS
A Nostoc punctiforme Sugar Transporter Necessary to Establish a Cyanobacterium-Plant SymbiosisThe major facilitator superfamily GlcP glucose permease of the symbiont cyanobacterium Nostoc punctiforme is necessary for infection of its plant partner, the hornwort Anthoceros punctatus.
A Major Latex-Like Protein Is a Key Factor in Crop Contamination by Persistent Organic PollutantsA latex-like protein binds and transports polychlorinated biphenyls into the aerial part of plants.
SIGNALING AND RESPONSE
Identification of Genes in Thuja plicata Foliar Terpenoid DefensesTranscriptome profiling of foliage with and without resin glands identifies candidate gene products in thujone biosynthesis.
Disease Resistance Gene-Induced Growth Inhibition Is Enhanced by rcd1 Independent of Defense Activation in ArabidopsisGrowth inhibition induced by a disease resistance gene is uncoupled from plant defense activation.
The Epiphytic Fungus Pseudozyma aphidis Induces Jasmonic Acid- and Salicylic Acid/Nonexpressor of PR1-Independent Local and Systemic ResistanceAn epiphytic fungus induces plant resistance against pathogens.
The Anticipation of Danger: Microbe-Associated Molecular Pattern Perception Enhances AtPep-Triggered Oxidative BurstMicrobial elicitors and the plant defense hormone jasmonic acid differentially modulates the plant’s innate immune response.
Identification of a Dual-Targeted Protein Belonging to the Mitochondrial Carrier Family That Is Required for Early Leaf Development in RiceA novel mitochondrial carrier protein is required for early leaf development in rice.
Phosphorylation of HopQ1, a Type III Effector from Pseudomonas syringae, Creates a Binding Site for Host 14-3-3 ProteinsA pathogen effector protein undergoes specific phosphorylation and associates with host 14-3-3 proteins, which affects its subcellular distribution and stability.
The Pseudomonas syringae Effector HopQ1 Promotes Bacterial Virulence and Interacts with Tomato 14-3-3 Proteins in a Phosphorylation-Dependent MannerA bacterial effector is delivered into plant cells during infection, is phosphorylated, and binds plant 14-3-3 proteins in a phosphorylation-dependent manner.
Interplay between Heat Shock Proteins HSP101 and HSA32 Prolongs Heat Acclimation Memory Posttranscriptionally in ArabidopsisPositive feedback between two heat shock proteins, and the consequent delayed degradation of one, extends tolerance to temperature extremes.
Conditional Involvement of CONSTITUTIVE PHOTOMORPHOGENIC1 in the Degradation of Phytochrome ALight-induced reduction of phyA levels depends on cullin1, while COP1 only regulates this process in specific conditions.
Heterotrimeric G Proteins Serve as a Converging Point in Plant Defense Signaling Activated by Multiple Receptor-Like KinasesArabidopsis heterotrimeric G proteins function as a converging point of plant defense signaling by mediating responses initiated by multiple receptor-like kinases, which may fulfill equivalent roles to GPCRs in fungi and animals.
Lipoxygenase6-Dependent Oxylipin Synthesis in Roots Is Required for Abiotic and Biotic Stress Resistance of ArabidopsisA defect in lipoxygenase6 abolishes production of jasmonates in roots and renders plants more susceptible to drought and crustacean feeding.
SYSTEMS AND SYNTHETIC BIOLOGY
Protein-Coding cis-Natural Antisense Transcripts Have High and Broad Expression in ArabidopsisProtein-coding overlapping genes in Arabidopsis have unexpectedly high levels and breadths of expression.
