Skip to main content

Main menu

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
    • Focus Collections
    • Classics Collection
    • Upcoming Focus Issues
  • Info for
    • Authors
    • Submit a Manuscript
    • Advertisers
    • Subscribers
    • Librarians
  • About
    • About the Journal
    • Editorial Board and Staff
  • More
    • Alerts
    • Contact Us
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Plant Cell Teaching Tools
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Physiology
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Plant Cell Teaching Tools
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Physiology

Advanced Search

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
    • Focus Collections
    • Classics Collection
    • Upcoming Focus Issues
  • Info for
    • Authors
    • Submit a Manuscript
    • Advertisers
    • Subscribers
    • Librarians
  • About
    • About the Journal
    • Editorial Board and Staff
  • More
    • Alerts
    • Contact Us
  • Follow plantphysiol on Twitter
  • Visit plantphysiol on Facebook
  • Visit Plantae
Research ArticleRESEARCH REPORTS
Open Access

What Is Stress? Dose-Response Effects in Commonly Used in Vitro Stress Assays

Hannes Claeys, Sofie Van Landeghem, Marieke Dubois, Katrien Maleux, Dirk Inzé
Hannes Claeys
Department of Plant Systems Biology, VIB, B–9052 Ghent, Belgium; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Hannes Claeys
Sofie Van Landeghem
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marieke Dubois
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Katrien Maleux
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dirk Inzé
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Dirk Inzé
  • For correspondence: dirk.inze@psb.vib-ugent.be

Published June 2014. DOI: https://doi.org/10.1104/pp.113.234641

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Figure 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 1.

    Literature study of in vitro stress assays. A, Concentrations of NaCl and mannitol/sorbitol used to impose salt and osmotic stress on Arabidopsis in PubMed Central open-access articles (n = 216). The median is indicated with the black lines. B, Phenotypes recorded to assess stress tolerance in PubMed Central open-access articles (n = 106). The different types of growth measurements are further broken down in the diagram on the right.

  • Figure 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 2.

    Sensitivity of germination, overall plant health, root length, and rosette area to abiotic stress. Root length (n = 20–24) and rosette area (n = 30–36) are expressed relative to nontreated plants. For germination rate and plant health (as scored by the appearance of visual stress symptoms, such as bleaching and anthocyanin accumulation), data come from three independent experiments. Error bars indicate se.

  • Figure 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 3.

    Effects of stress on rosette growth. A, C, E, and G, Projected rosette areas at 22 DAS (final time point), with representative images of plants for a range of mannitol, NaCl, and H2O2 concentrations. The top right insets show rosette compactness. For H2O2 treatment, the inset at the bottom left shows rosette areas (dots) and their averages (lines) grouped by the presence (red) or absence (black) of substantial bleaching. B, D, F, and H, RGRs as a function of the stress levels at 8 DAS (red), 15 DAS (blue), and 22 DAS (green). At NaCl concentrations greater than 150 mm (E and F), germination or seedling establishment was inhibited, so no growth data could be obtained for these concentrations, as represented by the dashed lines on the graphs. Error bars indicate se. The letters above the error bars denote significance groups (ANOVA; P < 0.05; n = 30–36).

  • Figure 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 4.

    Effects of stress on the expression of selected marker genes. Levels of oxidative stress markers (NAC032 and AKR4C9), ABA markers (CYP707A3 and NCED3), dehydration markers (LEA5, RD29B, and DREB2A), and mild osmotic stress markers (ERF5, WRKY33, and MYB51) in complete seedlings are represented as log2 (fold change) compared with nonstressed plants. Significant changes (ANOVA; P < 0.05; n = 3) are indicated with asterisks.

PreviousNext
Back to top

Table of Contents

Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Plant Physiology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
What Is Stress? Dose-Response Effects in Commonly Used in Vitro Stress Assays
(Your Name) has sent you a message from Plant Physiology
(Your Name) thought you would like to see the Plant Physiology web site.
Citation Tools
What Is Stress? Dose-Response Effects in Commonly Used in Vitro Stress Assays
Hannes Claeys, Sofie Van Landeghem, Marieke Dubois, Katrien Maleux, Dirk Inzé
Plant Physiology Jun 2014, 165 (2) 519-527; DOI: 10.1104/pp.113.234641

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
What Is Stress? Dose-Response Effects in Commonly Used in Vitro Stress Assays
Hannes Claeys, Sofie Van Landeghem, Marieke Dubois, Katrien Maleux, Dirk Inzé
Plant Physiology Jun 2014, 165 (2) 519-527; DOI: 10.1104/pp.113.234641
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • RESULTS
    • DISCUSSION
    • MATERIALS AND METHODS
    • Acknowledgments
    • Footnotes
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

In this issue

Plant Physiology: 165 (2)
Plant Physiology
Vol. 165, Issue 2
Jun 2014
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Advertising (PDF)
  • Ed Board (PDF)
  • Front Matter (PDF)
View this article with LENS

More in this TOC Section

  • Gibberellin Induces an Increase in Cytosolic Ca2+ via a DELLA-Independent Signaling Pathway
  • Arabidopsis β-Amylase2 Is a K+-Requiring, Catalytic Tetramer with Sigmoidal Kinetics
  • A SUMO Ligase AtMMS21 Regulates the Stability of the Chromatin Remodeler BRAHMA in Root Development
Show more RESEARCH REPORTS

Similar Articles

Our Content

  • Home
  • Current Issue
  • Plant Physiology Preview
  • Archive
  • Focus Collections
  • Classic Collections
  • The Plant Cell
  • Plant Direct
  • Plantae
  • ASPB

For Authors

  • Instructions
  • Submit a Manuscript
  • Editorial Board and Staff
  • Policies
  • Recognizing our Authors

For Reviewers

  • Instructions
  • Journal Miles
  • Policies

Other Services

  • Permissions
  • Librarian resources
  • Advertise in our journals
  • Alerts
  • RSS Feeds

Copyright © 2018 by The American Society of Plant Biologists

Powered by HighWire