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Research ArticleSIGNALING AND RESPONSE
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S-Sulfhydration: A Cysteine Posttranslational Modification in Plant Systems

Ángeles Aroca, Antonio Serna, Cecilia Gotor, Luis C. Romero
Ángeles Aroca
Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas y Universidad de Sevilla, 41092 Seville, Spain (A.A., C.G., L.C.R.); and
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  • ORCID record for Ángeles Aroca
Antonio Serna
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Cecilia Gotor
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Luis C. Romero
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  • For correspondence: lromero@ibvf.csic.es

Published May 2015. DOI: https://doi.org/10.1104/pp.15.00009

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  • © 2015 American Society of Plant Biologists. All Rights Reserved.

Abstract

Hydrogen sulfide is a highly reactive molecule that is currently accepted as a signaling compound. This molecule is as important as carbon monoxide in mammals and hydrogen peroxide in plants, as well as nitric oxide in both eukaryotic systems. Although many studies have been conducted on the physiological effects of hydrogen sulfide, the underlying mechanisms are poorly understood. One of the proposed mechanisms involves the posttranslational modification of protein cysteine residues, a process called S-sulfhydration. In this work, a modified biotin switch method was used for the detection of Arabidopsis (Arabidopsis thaliana) proteins modified by S-sulfhydration under physiological conditions. The presence of an S-sulfhydration-modified cysteine residue on cytosolic ascorbate peroxidase was demonstrated using liquid chromatography-tandem mass spectrometry analysis, and a total of 106 S-sulfhydrated proteins were identified. Immunoblot and enzyme activity analyses of some of these proteins showed that the sulfide added through S-sulfhydration reversibly regulates the functions of plant proteins in a manner similar to that described in mammalian systems.

  • Glossary

    H2S
    hydrogen sulfide
    NO
    nitric oxide
    H2O2
    hydrogen peroxide
    PTP1B
    Tyr phosphatase 1B
    BSM
    biotin switch method
    MMTS
    methyl methanethiosulfonate
    biotin-HPDP
    N-[6-(biotinamido)hexyl]-3′-(2'-pyridyldithio)-propionamide
    GAPDH
    glyceraldehyde 3-phosphate dehydrogenase
    DTT
    dithiothreitol
    LC
    liquid chromatography
    MS/MS
    tandem mass spectrometry
    • Received January 8, 2015.
    • Accepted March 24, 2015.
    • Published March 25, 2015.
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    S-Sulfhydration: A Cysteine Posttranslational Modification in Plant Systems
    Ángeles Aroca, Antonio Serna, Cecilia Gotor, Luis C. Romero
    Plant Physiology May 2015, 168 (1) 334-342; DOI: 10.1104/pp.15.00009

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    S-Sulfhydration: A Cysteine Posttranslational Modification in Plant Systems
    Ángeles Aroca, Antonio Serna, Cecilia Gotor, Luis C. Romero
    Plant Physiology May 2015, 168 (1) 334-342; DOI: 10.1104/pp.15.00009
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    May 2015
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