Article Figures & Data
Figures
- Figure 1.
LOX2 is required to induce JAZ10 expression in roots after cotyledon wounding. qRT-PCR of JAZ10 expression basally (A) and 1 h after cotyledon wounding (B) in 5-d-old aerial organs and roots of lox2, lox34, lox6, lox26, and lox346 mutants. JAZ10 transcript levels were normalized to those of UBC21 and are displayed relative to the expression of wild-type controls that are set to one and indicated with dashed lines. Bars represent the means of three biological replicates (±sd), each containing a pool of organs from approximately 60 seedlings. Complete qRT-PCR data are in Supplemental Data Set S1, data set 1. JGP reporter activity in control (C) and wounded wild-type (WT) and lox2 (D) 5-d-old seedlings. E, LOX2p:GUS reporter in control and wounded wild-type seedlings. Detection of GUS activity was performed 2 h after wounding. Orange asterisks indicate cotyledon wounding sites. Bars = 0.5 mm.
- Figure 2.
Evidence for axial shoot-to-root transport of jasmonate (or jasmonate precursors). JAZ10p:GUS reporter activity in grafted 13-d-old seedlings of the indicated scion/rootstock genotypes: WT/WT grafts (A and B), aos/aos grafts (C and D), aos/WT grafts (E and F), and WT/aos grafts (G and H). One of the two cotyledons was excised before grafting. GUS staining was performed 3 h after wounding aerial organs with a needle at sites indicated by orange asterisks (B, D, F, and H). Note the presence of reporter activity in the rootstock of the wild-type (WT)/aos grafted plant. Black arrowheads indicate grafting sites, and red arrowheads indicate reporter activity in the root. Bars = 1 mm.
- Figure 3.
LOX6 activity is sufficient to activate near wild-type JAZ10 expression in wounded and distal leaves of adult rosette plants. qRT-PCR of JAZ10 expression in unwounded L8 (A) and 1 h after wounding L8 (B) and distal L13 (C) in indicated genotypes. Basal JAZ10 levels are similar between L8 and L13 (Mousavi et al., 2013); thus, L8 was used as the unwounded control. Note that, after wounding, the lox234 mutant harboring only LOX6 activity reaches near wild-type JAZ10 expression levels in both L8 and L13. JAZ10 transcript levels were normalized to those of UBC21 and are displayed relative to the expression of wild-type control (L8) or wounded (L8 or L13) levels that are set to one and indicated with dashed lines. Bars represent the means of three to four biological replicates (±sd) from individual 4.5-week-old plants. Complete qRT-PCR data are in Supplemental Data Set S1, data set 2.
- Figure 4.
LOX6-derived oxylipins are exported from the vasculature into the leaf blade. A to D, LOX6p:LOX6-GUS protein fusion activity in rosettes before (A) and after (B–D) wounding. E to L, JAZ10p:GUS activity in the wild type (WT) and lox234 triple mutant before (E and I) and after (F–H and J–L) wounding. In each case, L3 of 21-d-old plants was wounded (orange asterisks), and GUS staining was performed 6 h later. L4 from a wounded (W) plant is shown in C, G, and K, and details of the primary order (1°), secondary order (2°), and tertiary order (3°) veins in L4 are shown in D, H, and L. Bars = 0.2 cm (A, B, E, F, I, and J), 1 mm (C, G, and K), and 200 μm (D, H, and L).
- Figure 5.
Oxylipins produced in xylem contact cells activate JAZ10 expression throughout the vascular cylinder and in extraveinal cells. A and B, Cross sections through the midvein showing the localization of LOX6-GUS fusion protein expressed under the LOX6 promoter before (A) and after (B) wounding. C and D, JAZ10p:GUS activity in the wild type (WT) before (C) and after (D) wounding. E and F, JAZ10p:GUS activity in the lox234 triple mutant before (E) and after (F) wounding. L4 of 21-d-old plants was stained for GUS activity in control samples and 6 h after wounding L3. c, Xylem contact cell; m, mesophyll; p, phloem region including phloem parenchyma; v, xylem vessel. Bar = 50 μm.
Additional Files
Supplemental Data
Supplemental Figures and Dataset
Files in this Data Supplement:
- Supplemental Data - Supplemental Figures 1-8
- Supplemental Data - Supplemental Dataset
