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Open Access

Integrative Approaches for the Identification and Localization of Specialized Metabolites in Tripterygium Roots

B. Markus Lange, Justin T. Fischedick, Malte F. Lange, Narayanan Srividya, Dunja Šamec, Brenton C. Poirier
B. Markus Lange
Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, Washington 99164–6340 (B.M.L., J.T.F., N.S., D.Š., B.C.P.);
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  • ORCID record for B. Markus Lange
  • For correspondence: lange-m@wsu.edu
Justin T. Fischedick
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Malte F. Lange
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Narayanan Srividya
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Dunja Šamec
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Brenton C. Poirier
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Published January 2017. DOI: https://doi.org/10.1104/pp.15.01593

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  • © 2017 American Society of Plant Biologists. All Rights Reserved.

Abstract

Members of the genus Tripterygium are known to contain an astonishing diversity of specialized metabolites. The lack of authentic standards has been an impediment to the rapid identification of such metabolites in extracts. We employed an approach that involves the searching of multiple, complementary chromatographic and spectroscopic data sets against the Spektraris database to speed up the metabolite identification process. Mass spectrometry-based imaging indicated a differential localization of triterpenoids to the periderm and sesquiterpene alkaloids to the cortex layer of Tripterygium roots. We further provide evidence that triterpenoids are accumulated to high levels in cells that contain suberized cell walls, which might indicate a mechanism for storage. To our knowledge, our data provide first insights into the cell type specificity of metabolite accumulation in Tripterygium and set the stage for furthering our understanding of the biological implications of specialized metabolites in this genus.

  • Glossary

    MSI
    mass spectrometry-based imaging
    MALDI
    matrix-assisted laser-desorption ionization
    LDI
    laser-desorption ionization
    QTOF
    quadrupole time of flight
    AMT
    accurate mass time
    MS/MS
    tandem mass spectrometry
    DAD
    diode array detector
    LOQ
    limit of quantitation
    MS
    mass spectrometry
    CLSM
    confocal laser-scanning microscopy
    RSD
    relative sd
    • Received October 14, 2016.
    • Accepted November 13, 2016.
    • Published November 18, 2016.

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    Integrative Approaches for the Identification and Localization of Specialized Metabolites in Tripterygium Roots
    B. Markus Lange, Justin T. Fischedick, Malte F. Lange, Narayanan Srividya, Dunja Šamec, Brenton C. Poirier
    Plant Physiology Jan 2017, 173 (1) 456-469; DOI: 10.1104/pp.15.01593

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    Integrative Approaches for the Identification and Localization of Specialized Metabolites in Tripterygium Roots
    B. Markus Lange, Justin T. Fischedick, Malte F. Lange, Narayanan Srividya, Dunja Šamec, Brenton C. Poirier
    Plant Physiology Jan 2017, 173 (1) 456-469; DOI: 10.1104/pp.15.01593
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