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Calcium Pumps and Interacting BON1 Protein Modulate Calcium Signature, Stomatal Closure, and Plant Immunity

Dong-Lei Yang, Zhenying Shi, Yongmei Bao, Jiapei Yan, Ziyuan Yang, Huiyun Yu, Yun Li, Mingyue Gou, Shu Wang, Baohong Zou, Dachao Xu, Zhiqi Ma, Jitae Kim, Jian Hua
Dong-Lei Yang
aState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
bSchool of Integrative Plant Science, Plant Biology Section, Cornell University, Ithaca, New York 14853
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  • ORCID record for Dong-Lei Yang
  • For correspondence: jh299@cornell.edu dlyang@njau.edu.cn
Zhenying Shi
bSchool of Integrative Plant Science, Plant Biology Section, Cornell University, Ithaca, New York 14853
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Yongmei Bao
aState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
bSchool of Integrative Plant Science, Plant Biology Section, Cornell University, Ithaca, New York 14853
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Jiapei Yan
bSchool of Integrative Plant Science, Plant Biology Section, Cornell University, Ithaca, New York 14853
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Ziyuan Yang
aState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
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Huiyun Yu
bSchool of Integrative Plant Science, Plant Biology Section, Cornell University, Ithaca, New York 14853
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Yun Li
aState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
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Mingyue Gou
bSchool of Integrative Plant Science, Plant Biology Section, Cornell University, Ithaca, New York 14853
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Shu Wang
bSchool of Integrative Plant Science, Plant Biology Section, Cornell University, Ithaca, New York 14853
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Baohong Zou
aState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
bSchool of Integrative Plant Science, Plant Biology Section, Cornell University, Ithaca, New York 14853
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Dachao Xu
aState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
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Zhiqi Ma
aState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
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Jitae Kim
bSchool of Integrative Plant Science, Plant Biology Section, Cornell University, Ithaca, New York 14853
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Jian Hua
aState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
bSchool of Integrative Plant Science, Plant Biology Section, Cornell University, Ithaca, New York 14853
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  • For correspondence: jh299@cornell.edu dlyang@njau.edu.cn

Published September 2017. DOI: https://doi.org/10.1104/pp.17.00495

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Abstract

Calcium signaling is essential for environmental responses including immune responses. Here, we provide evidence that the evolutionarily conserved protein BONZAI1 (BON1) functions together with autoinhibited calcium ATPase10 (ACA10) and ACA8 to regulate calcium signals in Arabidopsis. BON1 is a plasma membrane localized protein that negatively regulates the expression of immune receptor genes and positively regulates stomatal closure. We found that BON1 interacts with the autoinhibitory domains of ACA10 and ACA8, and the aca10 loss-of-function (LOF) mutants have an autoimmune phenotype similar to that of the bon1 LOF mutants. Genetic evidences indicate that BON1 positively regulates the activities of ACA10 and ACA8. Consistent with this idea, the steady level of calcium concentration is increased in both aca10 and bon1 mutants. Most strikingly, cytosolic calcium oscillation imposed by external calcium treatment was altered in aca10, aca8, and bon1 mutants in guard cells. In addition, calcium- and pathogen-induced stomatal closure was compromised in the aca10 and bon1 mutants. Taken together, this study indicates that ACA10/8 and BON1 physically interact on plasma membrane and function in the generation of cytosol calcium signatures that are critical for stomatal movement and impact plant immunity.

Calcium ion (Ca2+) is an important cellular second messenger for diverse developmental processes and environmental responses in both plants and animals (Lewis, 2001; Dodd et al., 2010; Kudla et al., 2010). The extreme low calcium concentration in cytosol (100–200 nm) creates a unique environment where calcium concentration can be regulated dynamically (Bush, 1995). Increases of calcium transient in cytosol are activated in various environmental and developmental processes, including root growth, stomatal movement, pollen growth, abiotic stress responses, and plant-microbe interaction. Calcium spiking with unique magnitude, frequency, shape, and duration in response to environmental and endogenous cues is referred as “calcium signature” and is thought to encode stimulus-specific information. It is shown that, in the guard cell where calcium is a key signal for stomatal movement control (Murata et al., 2015), calcium oscillation is indeed essential for stomatal closure (Allen et al., 2000).

The stimulus-specific calcium signature is thought to be generated by coordinated action of various Ca2+ influx and efflux systems including channels, pumps, and exchangers located at different cellular membranes. However, the molecular identities of calcium-specific influx channels remain controversial (Steinhorst and Kudla, 2013). Plasma membrane (PM)-localized cyclic nucleotide-gated channels, Glu receptor-like and TWO-PORE CHANNEL are considered as main channels that release Ca2+ in plant (Dodd et al., 2010; Kudla et al., 2010). Extrusion of Ca2+ from cytosol to external cell and intercellular store compartment is believed to be achieved by Ca2+-ATPases (Ca2+ pumps) and Ca2+/H+ antiporters driven by ATP and proton motive force, respectively (Geisler et al., 2000; Sze et al., 2000; Shigaki and Hirschi, 2006). Ca2+ pumps fall into two groups: type IIA, or ECA, for ER-type Ca2+-ATPases, and type IIB, or ACA, for autoinhibited Ca2+-ATPase (Geisler et al., 2000). ACAs have an N-terminal calmodulin-binding autoinhibitory domain that inhibits the ATPase activity in the C terminus, and they are localized to diverse membrane compartments (Sze et al., 2000; Boursiac and Harper, 2007). Calcium pumps in vacuole, ER, and nucleus were found to be important for calcium signal generation in response to environmental stimuli. The loss of function (LOF) of PCA1, a vacuole-localized ACA, resulted in sustained rather than transient elevated Ca2+ in cytosol under salt treatment in the moss Physcomitrella patens (Qudeimat et al., 2008). In tobacco (Nicotiana benthamiana), knockdown of NbCA1, an ER-localized ACA, greatly increased the amplitude and duration of calcium spikes induced by cryptogein (Zhu et al., 2010). The LOF of MCA, a nucleus-localized EAC, resulted in greatly reduced nuclear calcium spiking in response to Nod factor in Medicago truncatula (Capoen et al., 2011). The involvement of PM localized calcium pumps in calcium signal is not known yet in plants.

Calcium signaling has recently been genetically connected with plant immunity. Distinct calcium signatures are rapidly induced upon pathogen invasion (Lecourieux et al., 2006; Ma and Berkowitz, 2007). Disruption of putative calcium channels such as PM-localized cyclic nucleotide-gated channels and Glu receptor-like either enhance or compromise plant immune responses (Clough et al., 2000; Forde and Roberts 2014). The PM-localized calcium pumps ACA8 and ACA10 are found to be associated with immune receptor FLS2 and their LOF mutants were susceptible to bacterial pathogens (Frei dit Frey et al., 2012). Multiple calcium-binding proteins are also involved in plant immunity regulation. For instance, a calmodulin (CaM)-binding protein MLO negatively regulates resistance to powdery mildew in barley (Kim et al., 2002), and a Ca2+- and CaM-binding transcription factor AtSR1/CAMTA3 is a negative regulator of salicylic acid-dependent defense responses in Arabidopsis (Arabidopsis thaliana; Du et al., 2009). A CaM gene in N. benthamiana is required for immune responses triggered by silencing of the ER calcium pump CA1 (Zhu et al., 2010), and overexpression of a pepper CaM gene induces programmed cell death and enhances disease resistance (Choi et al., 2009). A chloroplast calcium-regulated protein, CAS, also plays an important role in plant immunity (Nomura et al., 2012). In addition, calcium-dependent protein kinases (CDPK or CPK) are critical regulators of plant immune responses both to pathogen-associated molecular patterns (PAMPs) and effectors (Boudsocq and Sheen, 2013). Four CDPKs (CPK4/5/6/11) are found to be critical for transcriptional reprogramming and reactive oxygen species production in responses to PAMPs (Boudsocq et al., 2010). CPK1/2/4/5/6/11 are shown to be involved in downstream events, including transcriptional reprogramming and reactive oxygen species production after activation of plant immune receptor NOD1-like Receptor (NLR) genes in response to pathogen effectors (Gao et al., 2013). Recently, CPK28 is shown to phosphorylate BIK1, a substrate of multiple PAMP receptors, and therefore attenuating PAMP signaling (Monaghan et al., 2014).

One intriguing component involved in calcium signaling and plant immunity is the Arabidopsis BON1 gene. BON1 is a member of an evolutionarily conserved copine family found in protozoa, plants, nematodes, and mammals (Creutz et al., 1998). The copine proteins have two calcium-dependent phospholipid-binding C2 domains at their amino (N) terminus and a putative protein-protein interaction von Willebrand A or A domain at their carboxyl (C) terminus (Rizo and Südhof, 1998; Whittaker and Hynes, 2002). The BON1 protein resides on the PM mainly through myristoylation of its second residue Gly (Hua et al., 2001; Li et al., 2010). Mutating residue Gly (G) 2 to Ala (A) abolishes PM localization of BON1 and results in the loss of BON1 activity (Li et al., 2010). BON1 has conserved Asp residues important for calcium binding in the two C2 domains. Mutating all these Asp residues in either C2A or C2B domains abolishes BON1 activity in rescuing the bon1-1 defects indicating that calcium binding is required for BON1 function (Li et al., 2010).

BON1 is a negative regulator of plant immune responses and a positive regulator of stomatal closure response. In the LOF mutant bon1-1 in Col-0 background, a plant immune receptor NLR gene suppressor of NPR1, constitutive1 (SNC1) is increased at transcript level through initial up-regulation followed by salicylic acid-mediated amplification (Yang and Hua, 2004; Li et al., 2007). The bon1-1 mutant thus has constitutive defense (autoimmune) responses and is consequently dwarf. However, these observed defects are dependent on a Col-0 specific NB-LRR (Nucleotide Binding-Leucine Rich Repeat) gene, SNC1, and the LOF bon1-2 allele in the Wassilewakija (Ws) accession does not exhibit constitutive defense activation because there is no functional SNC1 in Ws (Yang and Hua, 2004). In addition to the function in negatively regulating SNC1, BON1 has been recently shown to promote stomatal closure in response to stimuli, including abscisic acid and bacterial pathogen (Gou et al., 2015). This function is independent of SNC1 or NB-LRR signaling, and the bon1 mutant is defective in stomatal closure when autoimmunity is suppressed by the SNC1 LOF mutation snc1-11 (Gou et al., 2015). Therefore, BON1 has a more general role in regulating signaling events in addition to NLR gene expression.

It is intriguing that an evolutionarily conserved calcium-binding copine protein can regulate stomatal closure and impact NLR gene expression. Here, we identified calcium pumps ACA10 and ACA8 as interacting proteins of BON1 both physically and genetically. The LOF mutants of these genes have altered calcium signatures and calcium homeostasis and are defective in stomatal closure and plant immunity. Thus, we have uncovered a critical role for PM-localized calcium pumps ACA10/8 in calcium signature generation as well as their regulation by an evolutionarily conserved BON1 protein in Arabidopsis.

RESULTS

BON1 and the Calcium ATPase ACA10 Interact Physically

To further understand how BON1 functions in immunity, we searched for BON1 coexpressed genes, as they might function together with BON1. Among the top 300 genes with high coexpression values in the ATTED-II database (Obayashi et al., 2009), 33 genes were annotated as calcium-binding proteins including calcium channels, pumps, calmodulin, and calcium-dependent kinases (Supplemental Table S1). Five of them encode calcium efflux pumps, including ACA1, ACA2, ACA10, ACA11, and ACA.1. Previously, ACA.1 was identified as a putative BON1-interacting protein by BON1 immunoprecipitation followed by mass spectrometry (Gou et al., 2015), suggesting that BON1 could physically interact with ACA proteins. Because ACA1, 2, and 11 are localized to the plastid envelope, ER, and vacuole, respectively (Huang et al., 1993; Harper et al., 1998; Lee et al., 2007), we chose the PM-localized ACA10 for further analysis because BON1 is PM localized. This is also due to the fact that LOF mutant of ACA10 in the Nossen-0 (No-0) accession had a phenotype reminiscent to that of bon1 (see below section).

We first analyzed potential physical interaction between BON1 and ACA10. We were not able to clone the full length of ACA10 cDNA without mutations in E. coli, which was also reported for the full-length cDNAs of ACA9, the close homolog of ACA10 (Schiøtt et al., 2004). We therefore used a cDNA/genomic DNA chimera (where the genomic fragment is used after the 22nd exon) for ACA10 for expression analysis. When expressed in N. benthamiana, the ACA10-GFP fusion protein showed signals only on the PM (Supplemental Fig. S1), confirming that ACA10 is indeed a PM-localized protein (Bonza et al., 2000; Schiøtt et al., 2004). Bimolecular fluorescence complementation (BiFC; Bracha-Drori et al., 2004) assays were used to determine the interaction of BON1 and ACA10 in N. benthamiana. Strong fluorescence signals were observed when both the ACA10 fusion with N terminus of YFP and the BON1 fusion with C terminus of YFP were expressed (Fig. 1A; Supplemental Fig. S2); and the signals were on the PM, where both BON1 and ACA10 are localized (Supplemental Fig. S1A). The positive BiFC signal of ACA10 with BON1 is not a nonspecific interaction of BON1 with any PM protein, as another PM protein oligopeptide transporter3 (OPT3) did not give a positive signal when coexpressed with BON1 (Fig. 1A; Supplemental Fig. S2).

Figure 1.
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Figure 1.

Protein-protein interaction between ACA10 and BON1. A, BiFC assay of ACA10 and BON1 interaction. ACA10 fused with N-terminal part of YFP (ACA10-NE) and BON1 fused with C-terminal part of YFP (BON1-CE) were transiently expressed in N. benthamiana by Agrobacterium-mediated transformation. A control plasma membrane protein OPT3 coexpressed with BON1 is shown at the bottom. Bar = 50 µm. B, BiFC assay of interaction between BON1 and each of the five segments of ACA10: I, II, III, IV, and V (Supplemental Fig. S1B). Bar = 50 µm. C, Split-LUC assay of interaction of BON1 and the first segment of ACA10. Fusion of segment I of ACA10 with C terminus LUC (ACA10I-Cluc) was coexpressed with fusion of BON1 with N terminus LUC (BON1-Nluc) in N. benthamiana (upper left). Coexpressions of ACA10I-Cluc with Nluc (upper right), Cluc with BON1-Nluc (lower left), and Cluc with Nluc (lower right) were used as controls. D, Yeast-two-hybrid assay between the first segment of ACA10 and the A domain of BON1. BAP1 was used as a positive control. Shown is growth of serial dilutions of yeasts on synthetic medium without Leu or Trp (SC-LT) or synthetic medium without Leu, Trp, adenine, or His (SC-LTAH). AD, Activation domain of GAL4; BD,DNA-binding domain of GAL4.

We subsequently used a BiFC assay to test which segment of ACA10 (Supplemental Fig. S1B) interacts with BON1 and found that BON1 interacts with the N-terminal segment I that includes the autoinhibited domain of ACA10 (Fig. 1B). Four other segments (II–IV) of ACA10 were also analyzed, but no positive signals were observed (Fig. 1B). Further tests will determine their expression levels in order to determine whether these segments interact with BON1 in addition to segment I. The interaction between BON1 and the segment I of ACA10 was analyzed further by split-LUC (luciferase) assay (Fig. 1C). Coexpression of the fusion of BON1 and the N-terminal LUC (BON1-NLUC) with the fusion of C-terminal LUC and segment I of ACA10 (CLUC-ACA10I) in N. benthamiana gave a strong luminescence signal from the LUC activity, while the controls without coexpressing the two proteins showed no luminescence (Fig. 1C). This interaction was further verified by the yeast two-hybrid assay where the C-terminal A domain of BON1 (BON1-A) was found to interact with the segment I of ACA10 (Fig. 1D). The full-length BON1 did not exhibit interaction with segment I (Fig. 1D), likely because the N-terminal part of BON1 targets to the PM and inhibits the fusion protein from going to the nucleus to activate gene expression. Because the segment I of ACA10 contains the putative autoinhibitory motif that confers autoinhibition of many ACA proteins (Bonza et al., 2000; Curran et al., 2000; Geisler et al., 2000), BON1 may regulate ACA10 activity by binding to this motif or its nearby sequences.

ACA10 Mutant in the Nossen-0 (No-0) Background Has an Autoimmune Phenotype

We found that ACA10, like BON1, is a negative regulator of plant immunity. An aca10 mutant in No-0 accession, named cif1-1, was reported to have a compact inflorescence (George et al., 2008). This aca10 mutant allele will be referred to as aca10-cif1 to be consistent with other mutant names. We suspected that the growth phenotype of aca10-cif1 is at least partially due to constitutive activation of immune responses induced by plant immune receptor NB-LRR genes based on the following observations. Under long-day growth conditions, the young leaves of aca10-cif1 display a water-soaked phenotype that is similar to the autoimmune mutant bon1-1 (Fig. 2A; Yang and Hua 2004). In addition, the compact inflorescence phenotype of aca10-cif1 is reminiscent of that of bon1 bon2 bon3 pad4 mutant (Yang et al., 2006). Phenotype of water-soaked leaf was suppressed by a higher growth temperature of 28°C (Fig. 2A), reminiscent of the suppression of NB-LRR-mediated plant defense responses by an elevated temperature (Wang and Hua, 2009). Furthermore, the aca10-cif1 phenotype is only present in No-0, but not in Col-0 or Ws, which was due to an accession difference at the CIF2 locus (George et al., 2008). The CIF2 gene is not yet cloned, and it is likely to be a NB-LRR gene, as the accession specificity is often the property of such genes (Noël et al., 1999; Clark et al., 2007).

Figure 2.
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Figure 2.

The loss of ACA10 in Nossen-0 (No-0) background leads to autoimmune responses. A, Growth phenotype of aca10-cif1 and wild-type No-0 plants at 22°C and 28°C. B, Pst DC3000 bacterial growth in aca10-cif1 and No-0 plants under 22°C and 28°C. ** indicates significant difference by Student’s t test (P < 0.001). C, PR1 expression level in aca10-cif1 and No-0 plants at 22°C and 28°C analyzed by northern blotting. D, Growth phenotype of No-0, aca10-cif1, and aca10-cif1 pad4 plants grown at 22°C. E. PR1 expression level in No-0, aca10-cif1, and aca10-cif1pad4 plants analyzed by real-time RT-PCR assay. Actin2 is used as a control gene. ** indicates significant difference by Student’s t test (P < 0.001). dpi, Days postinoculation.

We therefore assayed the growth of virulent pathogen Pseudomonas syringe pv tomato (Pst) DC3000 in aca10-cif1. The bacterial growth was reduced by 10 times in the aca10-cif1 mutant compared to that in the wild-type No-0 plants at 22°C but not 28°C (Fig. 2B). This enhanced resistance likely results from a constitutive up-regulation of defense responses at 22°C. The defense marker gene PR1, which is induced by pathogen in the wild type, is up-regulated in aca10-cif1 in the absence of pathogen inoculation, and this up-regulation of PR1 expression was observed at 22°C but not at 28°C (Fig. 2C). To further test the hypothesis that growth defect is due to activation of NB-LRR-mediated defense, we introduced in aca10-cif1 mutant the LOF mutation in the PAD4 gene that plays an important role in such a defense (Wiermer et al., 2005). The pad4-1 mutant in Col-0 background was crossed to aca10-cif1 in No-0, and F2 plants were genotyped at the ACA10, CIF2, and PAD4 loci. All plants with genotypes of aca10-cif1 cif2-n (No-0 allele of CIF2) exhibited water-soaked leaf phenotype, while all aca10-cif1 cif2-n pad4 plants exhibited a wild-type phenotype (Fig. 2D). The suppression of cif1 mutant phenotype by pad4 is further confirmed by analyzing progenies from F2 plants with aca10-cif1 cif2-n pad4/+ genotype. In addition to the growth phenotype, pad4 mutation also reduced PR1 expression in aca10-cif1 (Fig. 2E). Taken together, ACA10 is a negative regulator of disease resistance in a PAD4-dependent manner in the No-0 accession, and constitutive immune responses result in growth retardation in aca10-cif1 mutant.

ACA10 and ACA8 Are Negative Regulators of Plant Immune Responses

An earlier study reported that ACA8, the closest homolog of ACA10, interacts with a PAMP receptor, FLS2, and is a positive regulator of plant immune response (Frei dit Frey et al., 2012). It was also reported that, using a surface inoculation method in which bacteria enter the plants through stomata, the aca8, aca10, and aca10aca8 mutants in the Col-0 background were more susceptible to virulent pathogen Pst DC3000 compared to the wild type Col-0 (Frei dit Frey et al., 2012). However, our characterization of the aca10-cif1 mutant as well as the genetic and physical interaction of ACA10 and BON1 indicate that ACA10 is a negative regulator of immune responses. To investigate this discrepancy, we analyzed the disease phenotype of aca10 and aca8 mutants in the Col-0 background. The single LOF mutants of aca10-2 or aca8-2 in Col-0 did not exhibit obvious growth defects in early developmental stage, but the double mutant had water-soaked leaves and exhibited dwarfism (Fig. 3, A and B). When analyzed for resistance to the virulent pathogen Pst DC3000, the aca10-2 and the aca10-2aca8-2 mutants consistently exhibited enhanced resistance compared to the wild-type Col-0 (Fig. 3, C–E). Using syringe infiltration and dipping methods, aca10-2 but not aca8-2 had reduced pathogen growth, while aca10 aca8 mutant had a further reduction of pathogen growth compared to the wild-type Col-0 (Fig. 3, C and D). We subsequently used the same spray method as described in the prior study (Frei dit Frey et al., 2012) for inoculation and found a smaller but significant reduction of pathogen growth in the aca10-2 and aca10-2aca8-2 mutants (Fig. 3E). We could not explain the discrepancy between our result and the prior result by different inoculation methods or plant growth conditions, although we cannot exclude the possibility that a difference in an undefined plant growth condition or pathogen preparation caused a difference in disease resistance. We conclude that ACA10 and ACA8 are negative regulators of defense responses to bacterial pathogen Pst DC3000 from our analysis of their mutants. This is supported by the up-regulation of a defense response marker gene PR1 in the aca10 and aca10aca8 mutants compared to the wild-type Col-0 (Fig. 3F).

Figure 3.
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Figure 3.

The growth and defense phenotypes of aca10 and aca8 mutant in Col-0 accession background. A, The growth phenotypes of Col-0, aca10-2, aca8-2, and aca10-2 aca8-2 at seedling stage. B, The growth phenotypes of Col-0, aca8-2, aca10-2, and aca10-2 aca8-2 at seed-setting stage. C to E, Growth of virulent pathogen Pst DC3000 in the above genotypes. Different inoculation methods were used: syringe infiltration (C), dipping (D), and spray (E). Different letters indicate statistical difference (P < 0.001 by Bonferroni test) among genotypes. F, PR1 expression level in Col-0, aca10, aca8, and aca10aca8 plants analyzed by real-time RT-PCR assay. Actin2 is used as a control gene. dpi, Days postinoculation.

Mutants of ACA10 and BON1 Have Synergistic Interactions

The physical interaction of BON1 and ACA10 proteins as well as a similar phenotype in immunity indicates that they might function together. To further test this hypothesis, we constructed double mutant of aca10 and bon1 in the Ws background, as neither of the LOF mutants aca10-1 nor bon1-2 in the Ws background exhibited visible developmental defects (Fig. 4A; Supplemental Fig. S3A). The double mutant had smaller, curled, and water-soaked leaves and produced a more compact inflorescence compared to the wild type or the single mutants at 22°C but not 28°C (Fig. 4A; Supplemental Fig. S3, A–D). Pathogen growth assay demonstrated that the disease resistance was increased in the aca10-1bon1-2 double mutant compared to wild type and single mutant under 22°C but not 28°C (Fig. 4B). The aca10-1bon1-2 double mutant had a significant increase of PR1 expression compared to wild type and the single mutants at 22°C but not 28°C (Fig. 4C). All the defects in the double mutant were suppressed by either pad4 or eds1. The two triple mutants aca10-1 bon1-2 eds1-1 and aca10-1 bon1-2 pad4-5, in contrast to aca10-1 bon1-2, had a wild-type leaf and inflorescence morphology (Fig. 4D; Supplemental Fig. S3E). These triple mutants also had the wild-type level of disease resistance and PR1 up-regulation (Fig. 4, E and F). Thus, the aca10-1 and bon1-2 has synergistic effect on immune responses in the Ws background. We hypothesize that NLR genes other than SNC1 are activated in the bon1-2 aca10-1 mutant in the Ws background similar to SNC1 activation in the bon1-1 mutant in the Col-0 background.

Figure 4.
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Figure 4.

Genetic interaction between BON1 and ACA10 in Ws background. A, Growth phenotypes of wild type, aca10-1, bon1-2, and aca10-1 bon1-2 in Ws background at 22°C and 28°C. B, Pst DC3000 bacterial growth in above genotypes assayed by the syringae infiltration method. C, PR1 expression level in above genotypes grown at 22°C and 28°C by RNA blotting. Same amount of total RNAs were loaded on gels and the blots were hybridized at the same time. D, Growth phenotypes of aca10-1 bon1-2 mutants and their eds1-1 and pad4-5 combination mutants grown at 22°C. E, Growth of Pst DC3000 in genotypes as in D assayed by the syringae infiltration method. F, PR1 expression level in genotypes as in D assayed by real-time RT-PCR. Genotypes: a/b = aca10-1bon1-2; a/b/eds1-1 = aca10-1bon1-2eds1-1; a/b/pad4-5 = aca10-1bon1-2pad4-5. Different letters in B and D indicate statistical difference (P < 0.001 by Bonferroni test) of various genotypes; dpi, Days postinoculation.

Overexpression of BON1 Suppressed the aca10-cif1 But not the aca10aca8 Defects

The synergistic genetic interaction between BON1 and ACA10 single mutants is not apparently consistent with the hypothesis that BON1 and ACA10 function in the same protein complex and/or regulate each other in a signaling pathway. However, this can be explained if members of the BON1 or the ACA10 family each have similar functions. In this case, overexpression of one family member of the upstream component might be able to compensate for the loss of one but not all family members of the downstream component. To test this hypothesis, we overexpressed BON1 in aca10-cif1 mutant. More than 20 transgenic lines were generated, and the majority of the lines showed a rescued phenotype with wild-type leaf and inflorescent morphology (Fig. 5A; Supplemental Fig. S4A). In addition, bacterial growth was increased and the PR1 expression was reduced in BON1-OE/aca10-cif1 compared to aca10-cif1 (Fig. 5, B and C).

Figure 5.
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Figure 5.

Overexpression of BON1 rescued defects of the aca10-cif1 mutant but not the aca10aca8 mutants. A, Growth phenotype of wild-type No-0, aca10-cif1, and BON1 overexpression in aca10-cif1 at 22°C. B, Growth of Pst DC3000 in genotypes above assayed by the dipping inoculation method. C, PR1 expression level in above genotypes by real-time RT-PCR assay. Actin2 is used as a control gene. D, Growth phenotype of aca10-2 aca8-2 (in Col-0) and BON1 overexpression in aca10aca8 at 22°C. E, Growth phenotype of BON1 overexpression in aca10aca8 double mutant in a mixed Col-0 and No-0 background. Shown as the second and the last plant, respectively, are the two parental lines used for the cross: the BON1-OE line in aca10-cif1 and the aca10-2 aca8-2 in Col-0. The two plants in the middle are F3s from the same F2 parent from the cross, with one carrying the BON1-OE transgene. Different letters in B and C indicate statistical difference (P < 0.001 by Bonferroni test) of various genotypes; dpi, Days postinoculation.

In contrast, when we overexpressed BON1 in the double mutant aca10aca8, none of the over 10 transgenic lines of BON1-OE/aca10aca8 exhibited any difference from the aca10aca8 mutant (Fig. 5D). To exclude the possibility that the nonrescue was due to lower expression of BON1 in aca10aca8 than in aca10-cif, we analyzed the BON1 protein level by protein blot. BON1 protein level was in general lower in BON1-OE/aca10aca8 than in BON1-OE/aca10-cif1; however, one BON1-OE/aca10-cif1 line exhibiting a rescued phenotype had a lower expression than several BON1-OE/aca10aca8 lines with a nonrescued phenotype (Supplemental Fig. S4B). To confirm the nonrescue phenotype in aca10aca8, we crossed a high BON1-expression line in aca10-cif1 (in No-0) with aca10aca8 (in Col-0) and analyzed the F2 progenies. The aca10aca8 plants (now in mixed No-0 and Col-0 background) exhibited a similar growth defect irrespective of the presence of the BON1-OE transgene. The nonrescuing of aca10aca8 defects by BON1-OE was further confirmed in the F3 progenies of several lines of BON1-OE/aca10aca8 in mixed background (Fig. 5E). Therefore, BON1-OE rescues the defects of aca10 single mutant, but not the aca10aca8 double mutant, which suggests that BON1 functions upstream of both ACA10 and ACA8.

ACA8 and BON1 Have Physical Interaction

We subsequently tested the hypothesis that BON1 physically interacts with ACA8 as well as ACA10. In the BiFC assay in N. benthamiana, coexpression of the full-length ACA8 and BON1 exhibited a strong fluorescence signal, while coexpression of BON1 and a control PM protein did not (Fig. 6A; Supplemental Fig. S2). The interaction between ACA8 and BON1 was verified by a positive signal when the N-terminal segment I of ACA8 and BON1 were coexpressed in the split-LUC assay (Fig. 6B). Therefore, both ACA8 and ACA10 interact with BON1, and the BON1-interacting domains in ACA8 and ACA10 are localized in the same region of the protein.

Figure 6.
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Figure 6.

Physical interaction of ACA8 and BON1. A, BiFC assay of ACA8 and BON1. ACA8 fused with N-terminal part of YFP (ACA8-NE) and BON1 fused with C-terminal part of YFP (BON1-CE) were transiently expressed in N. benthamiana by Agrobacterium-mediated transformation. The plasma membrane protein OPT3 was used as a negative control. Graphs show YFP-mediated fluorescence derived from the protein-protein interaction, chlorophyll autofluorescence (chlorophyll), and superimposed images of chlorophyll autofluorescence and YFP (Merge). Bar = 100 µm. B, Split-LUC assay of BON1 with N-terminal segment I of ACA8. Fusion of segment I of ACA8 with C terminus LUC (ACA8I-Cluc) was coexpressed with fusion of BON1 with N terminus LUC (BON1-Nluc) in N. benthamiana (upper left). Coexpressions of ACA8I-Cluc with Nluc (upper right), Cluc with BON1-Nluc (lower left), and Cluc with Nluc (lower right) were used as controls.

ACA10, ACA8, and BON1 Affect Calcium Homeostasis and Calcium Signals

Because ACA10 and ACA8 are calcium pumps, we hypothesize that the loss of the pump function or its regulation will alter calcium homeostasis. To test this hypothesis, we monitored calcium homeostasis and signature in plant cells using FRET reporter Yellow Cameleon (YC). A plant version of this calcium sensor, YC3.6, under the control of the constitutive 35S promoter (Yang et al., 2008), was introduced into aca10-2, aca8-2, and bon1-1 mutants. Steady levels of calcium were measured in guard cells where YC3.6 has a strong expression. Calcium concentration was measured higher in the aca10 mutants compared to the wild type in both the Ws and No-0 backgrounds (Fig. 7A), which is consistent with an earlier report based on another calcium reporter (Frei dit Frey et al., 2012). Interestingly, the bon1 mutants also exhibited an increase of calcium at steady status in both Ws and Col-0 backgrounds (Fig. 7A).

Figure 7.
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Figure 7.

Calcium homeostasis and calcium oscillation are altered in the bon1 and aca10 mutants. A, Steady-state calcium levels in guard cells of No-0, aca10-cif1, Ws, bon1-2, aca10-1, bon1-2 aca10-1, Col-0, and bon1-1 assayed by the YC3.6 reporter. Shown are the average and sd of ratios of FRET/CFP from at least 30 guard cells (from five plants, two leaves/plant, and three guard cells/leaf) of each genotype. ** and * indicate significant difference between the wild type and the mutant at P < 0.001 and P < 0.05, respectively, by Bonferroni test. B, Calcium signals induced by exogenous application of calcium in Col-0, bon1-1, aca10-2, and aca8-2 plants. Shown is the representative image of calcium signals in guard cells of each genotype measured as FRET/CFP ratio using the YC3.6 reporter. The two numbers underneath each genotype indicate the number of cells with the representative calcium pattern versus the number of total cells analyzed.

Furthermore, cytosolic calcium signals generated in response to imposed calcium were altered in the aca10 and bon1 mutants. In wild-type Col-0, an external application of 10 mm calcium rapidly induced cytosolic calcium oscillation in guard cells (Fig. 7B) as reported earlier (Allen et al., 2000; Hubbard et al., 2012). In aca10-2, aca8-2, and bon1-1 LOF mutants, only one calcium spike was observed, and no more calcium peaks followed (Fig. 7B). Moreover, the decreasing of calcium level from the first spike in the three mutants was drastically delayed compared to the wild-type Col-0 (Fig. 7B). Therefore, the initial influx of calcium ions happened normally, but the calcium oscillation pattern was lost. This observation indicates an essential role of BON1, ACA10, and ACA8 in cytosolic calcium oscillation and supports the hypothesis that BON1 functions closely with ACA10 and ACA8 in regulating calcium signature.

ACA10, ACA8, and BON1 Regulate Stomatal Movement

Calcium signaling is critical in controlling stomatal movement (Kim et al., 2010). The altered calcium signature in bon1, aca10, and aca8 guard cells suggests that these mutants might have defects in stomata closure in response to environmental stimuli. Indeed, the bon1 mutant does not close stomata in response to calcium, abscisic acid, or pathogen, and this defect is not a consequence of autoimmunity as it is independent of SNC1 or PAD4 (Gou et al., 2015; Fig. 8A). None of the aca10, aca8, and aca10aca8 mutants in No-0, Ws, or Col-0 closed their stomata in response to 10 mm calcium either (Fig. 8A). Furthermore, ACA10 and ACA8, similar to BON1, functions in pathogen induced stomata closure control as well. When applied with a coronatine-deficient (COR−) Pst DC3000 strain, which does not induce stomata reopening (Melotto et al., 2006), none of the aca10 and aca8 single or double mutants closed their stomata, in contrast to the wild type (Fig. 8B). We further examined the stomatal movement over a time course in response to Pst DC3000 COR− as well as Pst DC3000, which causes reclose of stomata in the wild-type Col-0. As expected, the wild-type Col-0 opened stomata at 1.5 h and 3 h in response to both Pst DC3000 COR− and Pst DC3000 and closed its stomata at 4.5 h when applied with Pst DC3000, but not Pst DC3000 COR− (Fig. 8C). In contrast, the aca8 aca10 mutant kept its stomata closed at 1.5, 3, and 4.5 h when applied with either of the pathogen strains, except for a slight opening at 3 h when applied with Pst DC3000 (Fig. 8C). This further supports that the aca10aca8 double mutant is insensitive or less sensitive to pathogens in stomatal closure compared to the wild type. Therefore, the BON1 and ACA10/8 regulate stomata closure and plant immune responses in a similar fashion, and they might do so through modulating calcium signatures.

Figure 8.
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Figure 8.

Stomatal movement is compromised in the bon1, aca10, and aca8 mutants. A, Stomatal closure induced by exogenous application of CaCl2 in wild type, aca10, aca8, and bon1 in No-0, Ws, and/or Col-0 backgrounds. B, Stomatal closure induced by exogenous application of Pst DC3000 COR− in wild type, aca10, and bon1 in the Col-0 background. C, Time course of stomatal movement by application of Pst DC3000 COR− and Pst DC3000 in the wild type and the aca8aca10 plants. ** and * indicate significant difference before and after treatment by Student’s t test at P < 0.001 and P < 0.05, respectively.

DISCUSSION

Calcium signaling is universal and complex. Despite rapid progress in deciphering calcium signaling and networks in various developmental and environmental responses, it remains largely unknown in many processes what calcium transporters are used to generate calcium signals and how these transporters are regulated. Here, we identified Ca2+-ATPases ACA10 and ACA8 and calcium-binding protein BON1 as important regulators of calcium signature (Fig. 9). They are essential to generate calcium oscillation in response to externally applied calcium, and without their functions, only one single calcium spike is produced instead of multiple repeated spikes. This could be due to a failure to produce additional spikes or, more likely, to terminate the first spike due to the lack of ACA10/8 function. Prior studies also found an effect of ACAs on calcium signatures. For instances, mutations of tonoplast-localized PCA in moss and ER-localized NbCA1 in tobacco caused elevated calcium concentration and increased the amplitude and duration of calcium spikes, respectively (Qudeimat et al., 2008; Zhu et al., 2010). Ca2+-ATPases are high-affinity but low-capacity transporters, whereas Ca2+-exchangers are low-affinity, high-capacity transporters. Based on their biochemistry characteristics, it is hypothesized that Ca2+-ATPase is the primary component that terminates Ca2+ signaling, whereas Ca2+-exchanger is the primary factor that removes Ca2+ following cytosolic Ca2+ elevation (Sze et al., 2000). The finding here that the loss of ACA10/8 function compromises cytosolic calcium oscillation not only establishes a critical role of PM-localized ACAs in calcium signals but also indicates a broader role of ACAs in calcium signature generation than previously thought.

Figure 9.
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Figure 9.

Working model for the role of ACA10/8 and BON1 in calcium signature and immunity responses. Extracelluar calcium was released into cytosol by plasma membrane localized calcium channels when the host cells use receptors to detect pathogen features. The transient increase of Ca2+ concentration activates BON1 and ACA10/8 complex, which pumps calcium from cytosol to extracellular space. This Ca2+ exclusion is necessary to activate the following Ca2+ increase in cytosol. Ca2+ oscillation in cytosol is generated coordinately by Ca2+ channels that released calcium from extracellular medium and subcellular compartments into cytosol and by Ca2+ pumps that export Ca2+ from the cytosol. The information within Ca2+ spiking was decoded by Ca2+ binding proteins and transmitted to control stomatal movement and the expression of defense response genes.

Environmental signals such as PAMPs trigger opening of calcium-permeable channels for calcium influx, leading to the cytosolic calcium spike and sometimes multiple spikes forming an oscillation (Thor and Peiter, 2014). Our study indicates that calcium pumps ACA10 and ACA8 are needed for the oscillation, but not the first spike. It is likely that calcium levels in cytosol are reset after the first induction, before subsequent spikes can be generated, and calcium pumps are needed for this resetting. BON1, whose activity is promoted by calcium binding (Li et al., 2010), potentially modulates calcium oscillation through its binding to and activating ACA10 and ACA8. ACA10 and ACA8 both have an autoinhibitory domain that overlaps with the calmodulin binding motif, and calmodulin binding is thought to activate ACAs (Tidow et al., 2012). The binding of BON1 to this autoinhibitory domain of ACA10/8 may alter the protein conformation of ACA10/8 or facilitate calmodulin binding of ACA10/8, leading to the release of autoinhibition of ACA10/8 (Fig. 9). Because BON1 and calmodulin are both calcium-binding proteins, the ACA10/8 pump activity could be controlled by calcium itself, thus forming a feedback regulation. Future analysis of the interaction among ACA10/8, BON1, and calmodulin will reveal further the regulatory mechanisms of calcium efflux conferred by ACA10/8.

Calcium oscillation is generated in response to a number of stimuli in plants, including NOD factor in a variety of legume species (Granqvist et al., 2015). In legume, calcium oscillation occurs in nucleus in response to NOD factor, and an ER- or nuclear envelope-localized calcium ATPase was found to be essential to generate nuclear calcium oscillation (Capoen et al., 2011). The finding of a role of PM-localized calcium ATPases in calcium oscillation in cytosol indicates that calcium ATPases have general roles in calcium-signature generation in various cellular compartments. Further study should determine whether ACA10/8 and BON1 affect calcium signatures in response to other stimuli as well. The role of BON1 in calcium-signature generation also has implications for the copine protein family, where BON1 is a member. Some copines in animals were identified as genetic modifiers of channel or receptor mutants (Church and Lambie, 2003; Gottschalk et al., 2005), and they have also been implicated in receptor-mediated signaling from cell culture analysis (Tomsig et al., 2004; Heinrich et al., 2010). It would be interesting to test if copines have a regulatory role of calcium signals involved in those processes.

This study also finds opposing functions of ACA10 and ACA8 in two layers of plant immunity, similar to BON1. On one hand, the aca10 and aca8 mutants are insensitive to calcium and pathogen in stomatal closure response, similar to the bon1 mutants. This feature presumably makes these mutant plants more susceptible to pathogen at the invasion stage. An earlier report did find a more susceptible phenotype for the aca8 and aca10 mutants (Frei dit Frey et al., 2012). However, we found in this study that the aca10 and aca10aca8 mutants have enhanced resistance when bacterial propagation in plants is assayed by either infiltration or surface inoculation methods. This discrepancy might have resulted from differences in unidentified plant or pathogen growth conditions because resistance phenotype was observed even when we replicated the experiment reported in Frei dit Frey et al. (2012). Nevertheless, the enhanced resistance we observed is temperature and PAD4 dependent, indicative of activation of plant immune receptor NLR genes reminiscent of the activation of NLR genes in the bon1 mutant. Apparently, this layer of regulation overrides the stomatal layer of immunity regulation as both bon1 and aca10 mutants have enhanced resistance measured by pathogen growth.

How do BON1 and ACA10/8 regulate two layers of plant immunity? Conceivably, their positive role in stomatal closure control is directly tied with their roles in calcium signature generating, and this might represent a more direct role of these proteins. Their roles in controlling pathogen growth likely results from their negative regulation of immune receptor gene expression, which could be associated with calcium homeostasis. Altered steady state of calcium might mimic signals from sustained pathogen invasion and therefore up-regulate or activate NLR genes. Indeed, disruption of calcium channels and pumps has been shown to affect plant immunity (Clough et al., 2000; Zhu et al., 2010). For instance, two vacuole-localized ACA members, ACA4 and ACA11, repress cell death and innate immunity in Arabidopsis (Boursiac et al., 2010). Although these molecules are located in different parts of the cell, including PM, ER, or vacuole, they could all regulate calcium levels in cytosol and perhaps nucleus as well.

Although both bon1 and aca10aca8 have autoimmunity, their growth phenotypes are not identical. This might be due to specific functions these proteins have in addition to their overlapping roles. It could also be due to overlapping functions among the BON1 family members or the ACA10 family members. BON1 has two other homologs, BON2 and BON3, in Arabidopsis, while ACA10 has ACA8 and ACA9 as close homologs as well as ACA12 and ACA13 as homologs. It is likely that BON1 family members interact with ACA10 family members, each pair with a different regulatory strength. Therefore, a partial loss of the family activity could result in different statuses of calcium homeostasis. This variation in calcium steady level might be associated with activation of different NLR genes. Identifying such NLR genes activated in various mutants of calcium channels and pumps will lead to a further understanding of the mode of action of calcium signatures as well as their roles in plant immunity.

MATERIALS AND METHODS

Arabidopsis (Arabidopsis thaliana) Mutants and Plant Growth

The seeds of aca10-cif1 and aca10-1 were kindly provided by R. Sharrock (George et al., 2008). The aca10-2 (GK-044H01) and aca8-2 (GK-688H09) lines were obtained from the Arabidopsis Stock Centre (http://arabidopsis.org). For growth phenotyping, the Arabidopsis plants were grown under constant light conditions with 100 mmol m−2 s−1 and relative humidity at 50% to 70%. For pathogen growth assays, plants were grown under 12 or 16 h light conditions. Nicotiana benthamiana plants were grown in the greenhouse at 24°C for 4 to 6 weeks before use for transient expression studies.

Protein Subcellular Localization Assay

An ACA10 genomic/cDNA hybrid was constructed and cloned into the Gateway (GW) entry vector pCR8 TOPO TA vector (Invitrogen). For localization of ACA10 protein, the ACA10 gene was transferred from the entry vector to the Hpt-psatn1-ACA10 (GW) vector and transformed into the Agrobacterium tumefaciens strain GV3101 for infiltration into the abaxial surface side of 4- to 6-week-old N. benthamiana plant leaves as previously described (Gou et al., 2015). Fluorescence of the epidermal cell layer of the lower leaf surface was examined at 2 to 4 d postinoculation. Images were captured by a Leica TCS SP2 Confocal microscope with excitation wavelength at 488 and 496 nm and emission wavelength between 520 and 535 nm for GFP signals.

BiFC Assay

The full-length cDNA fragments (without stop codon) of BON1, ACA8, OPT3 (Zhai et al., 2014), and ACA10 (genomic/cDNA hybrid) were amplified using primers in Supplemental Table S2 and cloned into the Gateway entry vectors pCR8 TOPO TA or pENTR/D TOPO (Invitrogen). For BiFC experiments, BON1 was cloned into pSPYCE-35S GW (Schütze et al., 2009) using LR clonase (Invitrogen) to generate BON1:cYFP constructs, while ACA8, ACA10, and OPT3 were cloned similarly into pSPYNE-35S GW to generate corresponding nYFP constructs, respectively. A previously described protocol (Schütze et al., 2009) was followed to observe BiFC signals with minor modification. The constructs were transformed into the A. tumefaciens strain GV3101. Overnight cell cultures were collected and resuspended in 1 mL of acetosyringone medium (10 mm MES-KOH, pH 5.6, 10 mm MgCl2, and 150 µm acetosyringone) to optical density at 600 nm (OD600) to 0.8. The working suspensions were prepared by mixing appropriate clones containing the BiFC constructs and the gene-silencing inhibitor pBA-HcPro plasmid (Menke et al., 2005) at 1:1:1 ratio and letting them stand for 2 to 4 h. The A. tumefaciens suspensions were then coinfiltrated into the abaxial surface of 4-week-old N. benthamiana plant leaves. Fluorescence of the epidermal cell layer of the lower leaf surface was examined at 3 to 4 d postinoculation. Images were captured by a Leica SP5 confocal microscope with excitation wavelength at 514 nm and emission wavelength between 500 and 550 nm for YFP signals.

Split-Luc Assay

The full-length cDNA of BON1 was amplified using the oligos listed in Supplemental Table S2. The PCR fragment of BON1 was ligated into the pCAMBIA-NLuc vector (Chen et al., 2008) digested by BamHI and SalI using the ClonExpress MultiS One Step Cloning Kit (Vazyme) to generate BON1-NLuc. The N-terminal parts of ACA8 and ACA10 were each amplified using the oligos listed in Supplemental Table S2. The PCR fragments of ACA8 and ACA10 were ligated into the pCAMBIA-CLuc vector (Chen et al., 2008) digested by KpnI and SalI using a similar strategy as above to generate ACA8I-CLuc and ACA10I-CLuc. These constructs were transformed into A. tumefaciens strain GV3101.

A. tumefaciens GV3101 strains containing recombinant constructs were grown in liquid Luria-Bertani medium with rifampicin and kanamycin for 2 d, pelleted, and resuspended in Murashige and Skoog with 10 mm MES medium containing 200 μm acetosyringone to a final concentration of OD600 = 0.6. After 2 h induction, the bacterial suspensions were infiltrated into young expanded leaves of N. benthamiana plants with a needleless syringe. After infiltration, the plants were covered with dark bags at 23°C for 48 h. The plants were then kept under light for 16 h, sprayed with 1 mm luciferin in 0.01% Triton X100 solution, and kept in dark for 5 min to quench the fluorescence. A deep cooling CCD imaging apparatus (DU934P-BV, Andor) was used to capture the fluorescence image. The camera was cooled to −80°C, and all images were taken after 3 min exposure.

Pathogen Growth Assay

Pst DC3000 grown on plates with King’s B medium were washed, collected, and diluted with 10 mm MgCl2 and 0.02% Silwet l-77. Syringe infiltration was used for inoculation unless stated otherwise, where dipping or spray methods were used. For syringe infiltration, bacteria were diluted to OD600 of 0.002 and syringe-infiltrated on leaves of 3- to 4-week-old plants. Six inoculated leaves were collected as one sample, weighed, ground in 1 mL of 10 mm MgCl2, and shaken at room temperature for 1 h. Dipping inoculation was performed as previously described (Gou et al., 2015), and bacteria were diluted to OD600 = 0.05 for dipping seedlings of 2 weeks old. Three whole seedlings were collected as one sample. Spray inoculation was performed as described previously (Frei dit Frey et al., 2012), and the bacteria were diluted to OD600 = 0.2 for spray.

To determine bacterial growth (for all inoculation methods), serial dilutions of the ground tissue solution were spotted on KB medium, and the number of colony forming units per fresh weight was calculated. Three to four samples were analyzed for each genotype and time point.

Yeast Two-Hybrid Assay

The yeast (Saccharomyces cerevisiae) two-hybrid constructs were made in the pDEST-GBKT7 and pDEST-GADT7 Gateway vectors (Rossignol et al., 2007). The DNA fragment of the N-terminal segment I of ACA10 was cloned into the pCR8 TOPO TA vector and transferred to the pDEST-GBKT7 vector to obtain the BD:ACA10-I construct. The AD:BON1, AD:BON1-A, and BD-BAP1 constructs were previously described (Hua et al., 2001). The yeast two-hybrid assay was performed as previously described (Hua et al., 2001; Li et al., 2010).

Northern Blotting and Real-Time RT-PCR

Total RNA was extracted from 3-week old seedlings using Trizol reagent (Invitrogen) as instructed. RNA of 20 μg was resolved in a 1.2% gel containing formaldehyde. RNA blots were hybridized with gene-specific and 32P-labeled single-strand DNA probe.

For real-time PCR, SuperScript II Reverse Transcriptase (Invitrogen) was used to synthesize cDNA from isolated RNA. Real-time RT-PCR was performed using PR1 primers listed in Supplemental Table S2. The ACTIN2 gene was used as internal controls. Advanced Universal SYBR Green Supermix (Bio-Rad) was used for real-time RT-PCR.

Western Blotting

The vector of 35S::BON1-HA used to overexpress BON1 was reported previously (Gou et al., 2015). Arabidopsis leaves grown in soil under constant light for 3 weeks were used for protein extraction and western blotting with anti-HA antibody (Sigma Aldrich, catalog: H3663) following a previously described method (Gou et al., 2015).

Stomatal Closure Assay

Stomatal aperture assay was performed as described (Nomura et al., 2012). Young rosette leaves from 17- to 25-d-old Arabidopsis plants were detached; floated on the stomatal opening buffer containing 10 mm Tris-MES (pH 6.15), 5 mm KCl, and 50 μm CaCl2; and incubated for 2.5 h under white light (200–250 μmol m−2 s−1) at 22°C. Each leaf was then clung onto a coverslip with a medical adhesive (Hollister), and their mesophyll cells were removed by a razor blade. The epidermal strips were then transferred into the stomatal closing buffer containing 10 mm Tris-MES (pH 6.15), 5 mm KCl and 10 mm CaCl2 and incubated for another 2 h under white light. Then the epidermal strips were observed under an inverted microscopy (model D1, Carl Zeiss) before and after the closing buffer treatment. The stomatal aperture was calculated as the ratio of the inner width/outer length of each pair of stomata. For each sample, more than 50 guard cells were calculated, and the experiments were repeated four times.

Calcium Level Measurement

For calcium oscillation experiments, the epidermal strips after the light treatment were acquired as described above. Coverslips were then placed in a perfusion chamber that was fit to an inverted microscopy (model D1, Carl Zeiss) equipped with an emission filter wheel (Lambda XL) and a CCD camera (Andor Technology). Imaging calcium in the guard cells was conducted by monitoring the ratio (535 nm/442 nm) of YC3.6 using the MetaFluor fluorescence ratio imaging software. The epidermal strips were first measured for approximately 100 s, and then the opening buffer was changed into the closing buffer by an injector when the same epidermal strips were measured for 700 s. The interval of image acquisition was 3 s. For each genotype, more than 30 guard cells were measured, and the experiments were repeated three times.

Measurement of steady level of Ca2+ concentration using the YC3.6 system was performed with ZESS LSM710 confocal laser-scanning microscope following the protocol previously described (Krebs et al., 2012).

Supplemental Data

The following supplemental materials are available.

  • Supplemental Figure S1. Subcellular localization and structure of the ACA10 protein.

  • Supplemental Figure S2. Physical interaction of ACA8/10 with BON1 assayed by BiFC.

  • Supplemental Figure S3. The growth defect of aca10-1 bon1-2 is dependent on temperature, EDS1, and PAD4.

Supplemental Figure S4. Overexpression of BON1 in the aca10 mutants.

  • Supplemental Table S1. List of BON1 coexpressed genes that code for calcium signaling molecules.

  • Supplemental Table S2. Primers used in this study.

Acknowledgments

The authors thank Y. Wang for the assistance in microscopy, Y. Tan for technical assistance on YC3.6 assay, R. Sharrock for the seeds of cif1-1 and aca10-2, and J. Schroeder for the seeds of 35S:YC3.6 transgenic plant.

Footnotes

  • www.plantphysiol.org/cgi/doi/10.1104/pp.17.00495

  • The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Jian Hua (jh299{at}cornell.edu).

  • J.H. and D.-L.Y. conceived the project; D.-L.Y., Z.S., Y.B., J.Y., and J.H. designed the experiments, analyzed the data, and made the figures; D.-L.Y., Z.S., Y.B., J.Y., Z.Y., H.Y., Y.L., M.G., S.W., B.Z., D.X., Z.M., and J.K. conducted experiments; J.H. and D.-L.Y. wrote the paper.

  • ↵1 This work was supported by grants from National Science Foundation (IOS-0919914 and IOS-1353738) to J.H., the National Key Research and Development Program of China (2016YFD0100600) to D.-L.Y., Natural Science Foundation of Jiangsu (BK20150659) to D.-L.Y., National Science Foundation of China (31330061) to B.Z., and Jiangsu Collaborative Innovation Center for Modern Crop Production to J.H. and D.-L.Y. The authors thank the China Scholarship Council for fellowships to B.Z., H.Y., and Y.B. and the Shanghai Institute of Plant Physiology and Ecology for a fellowship to Z.S.

  • ↵2 These authors contributed equally to the article.

  • ↵3 Current address: Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.

  • ↵4 Current address: Biology Department, Brookhaven National Laboratory, Upton, NY 11973.

  • ↵[OPEN] Articles can be viewed without a subscription.

Glossary

PM
plasma membrane
LOF
loss of function
PAMP
pathogen-associated molecular pattern
BiFC
bimolecular fluorescence complementation
  • Received April 17, 2017.
  • Accepted July 11, 2017.
  • Published July 12, 2017.

REFERENCES

  1. ↵
    1. Allen GJ,
    2. Chu SP,
    3. Schumacher K,
    4. Shimazaki CT,
    5. Vafeados D,
    6. Kemper A,
    7. Hawke SD,
    8. Tallman G,
    9. Tsien RY,
    10. Harper JF, et al.
    (2000) Alteration of stimulus-specific guard cell calcium oscillations and stomatal closing in Arabidopsis det3 mutant. Science 289: 2338–2342
    OpenUrlAbstract/FREE Full Text
  2. ↵
    1. Bonza MC,
    2. Morandini P,
    3. Luoni L,
    4. Geisler M,
    5. Palmgren MG,
    6. De Michelis MI
    (2000) At-ACA8 encodes a plasma membrane-localized calcium-ATPase of Arabidopsis with a calmodulin-binding domain at the N terminus. Plant Physiol 123: 1495–1506
    OpenUrlAbstract/FREE Full Text
  3. ↵
    1. Boudsocq M,
    2. Sheen J
    (2013) CDPKs in immune and stress signaling. Trends Plant Sci 18: 30–40
    OpenUrlCrossRefPubMed
  4. ↵
    1. Boudsocq M,
    2. Willmann MR,
    3. McCormack M,
    4. Lee H,
    5. Shan L,
    6. He P,
    7. Bush J,
    8. Cheng SH,
    9. Sheen J
    (2010) Differential innate immune signalling via Ca(2+) sensor protein kinases. Nature 464: 418–422
    OpenUrlCrossRefPubMed
  5. ↵
    1. Boursiac Y,
    2. Harper JF
    (2007) The origin and function of calmodulin regulated Ca2+ pumps in plants. J Bioenerg Biomembr 39: 409–414
    OpenUrlCrossRefPubMed
  6. ↵
    1. Boursiac Y,
    2. Lee SM,
    3. Romanowsky S,
    4. Blank R,
    5. Sladek C,
    6. Chung WS,
    7. Harper JF
    (2010) Disruption of the vacuolar calcium-ATPases in Arabidopsis results in the activation of a salicylic acid-dependent programmed cell death pathway. Plant Physiol 154: 1158–1171
    OpenUrlAbstract/FREE Full Text
  7. ↵
    1. Bracha-Drori K,
    2. Shichrur K,
    3. Katz A,
    4. Oliva M,
    5. Angelovici R,
    6. Yalovsky S,
    7. Ohad N
    (2004) Detection of protein-protein interactions in plants using bimolecular fluorescence complementation. Plant J 40: 419–427
    OpenUrlCrossRefPubMed
  8. ↵
    1. Bush DS
    (1995) Calcium regulation in plant cells and its role in signaling. Annu Rev Plant Physiol Plant Mol Biol 46: 95–122
    OpenUrlCrossRef
  9. ↵
    1. Capoen W,
    2. Sun J,
    3. Wysham D,
    4. Otegui MS,
    5. Venkateshwaran M,
    6. Hirsch S,
    7. Miwa H,
    8. Downie JA,
    9. Morris RJ,
    10. Ané JM, et al.
    (2011) Nuclear membranes control symbiotic calcium signaling of legumes. Proc Natl Acad Sci USA 108: 14348–14353
    OpenUrlAbstract/FREE Full Text
  10. ↵
    1. Chen H,
    2. Zou Y,
    3. Shang Y,
    4. Lin H,
    5. Wang Y,
    6. Cai R,
    7. Tang X,
    8. Zhou JM
    (2008) Firefly luciferase complementation imaging assay for protein-protein interactions in plants. Plant Physiol 146: 368–376
    OpenUrlAbstract/FREE Full Text
  11. ↵
    1. Choi HW,
    2. Lee DH,
    3. Hwang BK
    (2009) The pepper calmodulin gene CaCaM1 is involved in reactive oxygen species and nitric oxide generation required for cell death and the defense response. Mol Plant Microbe Interact 22: 1389–1400
    OpenUrlCrossRefPubMed
  12. ↵
    1. Church DL,
    2. Lambie EJ
    (2003) The promotion of gonadal cell divisions by the Caenorhabditis elegans TRPM cation channel GON-2 is antagonized by GEM-4 copine. Genetics 165: 563–574
    OpenUrlAbstract/FREE Full Text
  13. ↵
    1. Clark RM,
    2. Schweikert G,
    3. Toomajian C,
    4. Ossowski S,
    5. Zeller G,
    6. Shinn P,
    7. Warthmann N,
    8. Hu TT,
    9. Fu G,
    10. Hinds DA, et al.
    (2007) Common sequence polymorphisms shaping genetic diversity in Arabidopsis thaliana. Science 317: 338–342
    OpenUrlAbstract/FREE Full Text
  14. ↵
    1. Clough SJ,
    2. Fengler KA,
    3. Yu IC,
    4. Lippok B,
    5. Smith RK, Jr..,
    6. Bent AF
    (2000) The Arabidopsis dnd1 “defense, no death” gene encodes a mutated cyclic nucleotide-gated ion channel. Proc Natl Acad Sci USA 97: 9323–9328
    OpenUrlAbstract/FREE Full Text
  15. ↵
    1. Creutz CE,
    2. Tomsig JL,
    3. Snyder SL,
    4. Gautier MC,
    5. Skouri F,
    6. Beisson J,
    7. Cohen J
    (1998) The copines, a novel class of C2 domain-containing, calcium-dependent, phospholipid-binding proteins conserved from Paramecium to humans. J Biol Chem 273: 1393–1402
    OpenUrlAbstract/FREE Full Text
  16. ↵
    1. Curran AC,
    2. Hwang I,
    3. Corbin J,
    4. Martinez S,
    5. Rayle D,
    6. Sze H,
    7. Harper JF
    (2000) Autoinhibition of a calmodulin-dependent calcium pump involves a structure in the stalk that connects the transmembrane domain to the ATPase catalytic domain. J Biol Chem 275: 30301–30308
    OpenUrlAbstract/FREE Full Text
  17. ↵
    1. Dodd AN,
    2. Kudla J,
    3. Sanders D
    (2010) The language of calcium signaling. Annu Rev Plant Biol 61: 593–620
    OpenUrlCrossRefPubMed
  18. ↵
    1. Du L,
    2. Ali GS,
    3. Simons KA,
    4. Hou J,
    5. Yang T,
    6. Reddy AS,
    7. Poovaiah BW
    (2009) Ca(2+)/calmodulin regulates salicylic-acid-mediated plant immunity. Nature 457: 1154–1158
    OpenUrlCrossRefPubMed
  19. ↵
    1. Forde BG,
    2. Roberts MR
    (2014) Glutamate receptor-like channels in plants: A role as amino acid sensors in plant defence? F1000Prime Rep 6: 37
    OpenUrl
  20. ↵
    1. Frei dit Frey N,
    2. Mbengue M,
    3. Kwaaitaal M,
    4. Nitsch L,
    5. Altenbach D,
    6. Haweker H,
    7. Lozano-Duran R,
    8. Njo MF,
    9. Beeckman T,
    10. Huettel B, et al.
    (2012) Plasma membrane calcium ATPases are important components of receptor-mediated signaling in plant immune responses and development. Plant Physiol 159: 798–809
    OpenUrlAbstract/FREE Full Text
  21. ↵
    1. Gao X,
    2. Chen X,
    3. Lin W,
    4. Chen S,
    5. Lu D,
    6. Niu Y,
    7. Li L,
    8. Cheng C,
    9. McCormack M,
    10. Sheen J, et al.
    (2013) Bifurcation of Arabidopsis NLR immune signaling via Ca2+-dependent protein kinases. PLoS Pathog 9: e1003127
    OpenUrlCrossRefPubMed
  22. ↵
    1. Geisler M,
    2. Axelsen KB,
    3. Harper JF,
    4. Palmgren MG
    (2000) Molecular aspects of higher plant P-type Ca(2+)-ATPases. Biochim Biophys Acta 1465: 52–78
    OpenUrlCrossRefPubMed
  23. ↵
    1. George L,
    2. Romanowsky SM,
    3. Harper JF,
    4. Sharrock RA
    (2008) The ACA10 Ca2+-ATPase regulates adult vegetative development and inflorescence architecture in Arabidopsis. Plant Physiol 146: 716–728
    OpenUrlAbstract/FREE Full Text
  24. ↵
    1. Gottschalk A,
    2. Almedom RB,
    3. Schedletzky T,
    4. Anderson SD,
    5. Yates III JR,
    6. Schafer WR
    (2005) Identification and characterization of novel nicotinic receptor-associated proteins in Caenorhabditis elegans. EMBO J 24: 2566–2578
    OpenUrlAbstract/FREE Full Text
  25. ↵
    1. Gou M,
    2. Zhang Z,
    3. Zhang N,
    4. Huang Q,
    5. Monaghan J,
    6. Yang H,
    7. Shi Z,
    8. Zipfel C,
    9. Hua J
    (2015) Opposing effects on two phases of defense responses from concerted actions of HEAT SHOCK COGNATE70 and BONZAI1 in Arabidopsis. Plant Physiol 169: 2304–2323
    OpenUrlAbstract/FREE Full Text
  26. ↵
    1. Granqvist E,
    2. Sun J,
    3. Op den Camp R,
    4. Pujic P,
    5. Hill L,
    6. Normand P,
    7. Morris RJ,
    8. Downie JA,
    9. Geurts R,
    10. Oldroyd GE
    (2015) Bacterial-induced calcium oscillations are common to nitrogen-fixing associations of nodulating legumes and nonlegumes. New Phytol 207: 551–558
    OpenUrlCrossRefPubMed
  27. ↵
    1. Harper JF,
    2. Hong B,
    3. Hwang I,
    4. Guo HQ,
    5. Stoddard R,
    6. Huang JF,
    7. Palmgren MG,
    8. Sze H
    (1998) A novel calmodulin-regulated Ca2+-ATPase (ACA2) from Arabidopsis with an N-terminal autoinhibitory domain. J Biol Chem 273: 1099–1106
    OpenUrlAbstract/FREE Full Text
  28. ↵
    1. Heinrich C,
    2. Keller C,
    3. Boulay A,
    4. Vecchi M,
    5. Bianchi M,
    6. Sack R,
    7. Lienhard S,
    8. Duss S,
    9. Hofsteenge J,
    10. Hynes NE
    (2010) Copine-III interacts with ErbB2 and promotes tumor cell migration. Oncogene 29: 1598–1610
    OpenUrlCrossRefPubMed
  29. ↵
    1. Hua J,
    2. Grisafi P,
    3. Cheng SH,
    4. Fink GR
    (2001) Plant growth homeostasis is controlled by the Arabidopsis BON1 and BAP1 genes. Genes Dev 15: 2263–2272
    OpenUrlAbstract/FREE Full Text
  30. ↵
    1. Huang L,
    2. Berkelman T,
    3. Franklin AE,
    4. Hoffman NE
    (1993) Characterization of a gene encoding a Ca(2+)-ATPase-like protein in the plastid envelope. Proc Natl Acad Sci USA 90: 10066–10070
    OpenUrlAbstract/FREE Full Text
  31. ↵
    1. Hubbard KE,
    2. Siegel RS,
    3. Valerio G,
    4. Brandt B,
    5. Schroeder JI
    (2012) Abscisic acid and CO2 signalling via calcium sensitivity priming in guard cells, new CDPK mutant phenotypes and a method for improved resolution of stomatal stimulus-response analyses. Ann Bot (Lond) 109: 5–17
    OpenUrlCrossRefPubMed
  32. ↵
    1. Kim TH,
    2. Böhmer M,
    3. Hu H,
    4. Nishimura N,
    5. Schroeder JI
    (2010) Guard cell signal transduction network: advances in understanding abscisic acid, CO2, and Ca2+ signaling. Annu Rev Plant Biol 61: 561–591
    OpenUrlCrossRefPubMed
  33. ↵
    1. Kim MC,
    2. Panstruga R,
    3. Elliott C,
    4. Müller J,
    5. Devoto A,
    6. Yoon HW,
    7. Park HC,
    8. Cho MJ,
    9. Schulze-Lefert P
    (2002) Calmodulin interacts with MLO protein to regulate defence against mildew in barley. Nature 416: 447–451
    OpenUrlCrossRefPubMed
  34. ↵
    1. Krebs M,
    2. Held K,
    3. Binder A,
    4. Hashimoto K,
    5. Den Herder G,
    6. Parniske M,
    7. Kudla J,
    8. Schumacher K
    (2012) FRET-based genetically encoded sensors allow high-resolution live cell imaging of Ca2+ dynamics. Plant J 69: 181–192
    OpenUrlCrossRefPubMed
  35. ↵
    1. Kudla J,
    2. Batistic O,
    3. Hashimoto K
    (2010) Calcium signals: The lead currency of plant information processing. Plant Cell 22: 541–563
    OpenUrlAbstract/FREE Full Text
  36. ↵
    1. Lecourieux D,
    2. Ranjeva R,
    3. Pugin A
    (2006) Calcium in plant defence-signalling pathways. New Phytol 171: 249–269
    OpenUrlCrossRefPubMed
  37. ↵
    1. Lee SM,
    2. Kim HS,
    3. Han HJ,
    4. Moon BC,
    5. Kim CY,
    6. Harper JF,
    7. Chung WS
    (2007) Identification of a calmodulin-regulated autoinhibited Ca2+-ATPase (ACA11) that is localized to vacuole membranes in Arabidopsis. FEBS Lett 581: 3943–3949
    OpenUrlCrossRefPubMed
  38. ↵
    1. Lewis RS
    (2001) Calcium signaling mechanisms in T lymphocytes. Annu Rev Immunol 19: 497–521
    OpenUrlCrossRefPubMed
  39. ↵
    1. Li Y,
    2. Gou M,
    3. Sun Q,
    4. Hua J
    (2010) Requirement of calcium binding, myristoylation, and protein-protein interaction for the Copine BON1 function in Arabidopsis. J Biol Chem 285: 29884–29891
    OpenUrlAbstract/FREE Full Text
  40. ↵
    1. Li Y,
    2. Yang S,
    3. Yang H,
    4. Hua J
    (2007) The TIR-NB-LRR gene SNC1 is regulated at the transcript level by multiple factors. Mol Plant Microbe Interact 20: 1449–1456
    OpenUrlCrossRefPubMed
  41. ↵
    1. Ma W,
    2. Berkowitz GA
    (2007) The grateful dead: Calcium and cell death in plant innate immunity. Cell Microbiol 9: 2571–2585
    OpenUrlCrossRefPubMed
  42. ↵
    1. Melotto M,
    2. Underwood W,
    3. Koczan J,
    4. Nomura K,
    5. He SY
    (2006) Plant stomata function in innate immunity against bacterial invasion. Cell 126: 969–980
    OpenUrlCrossRefPubMed
  43. ↵
    1. Menke FL,
    2. Kang HG,
    3. Chen Z,
    4. Park JM,
    5. Kumar D,
    6. Klessig DF
    (2005) Tobacco transcription factor WRKY1 is phosphorylated by the MAP kinase SIPK and mediates HR-like cell death in tobacco. Mol Plant Microbe Interact 18: 1027–1034
    OpenUrlCrossRefPubMed
  44. ↵
    1. Monaghan J,
    2. Matschi S,
    3. Shorinola O,
    4. Rovenich H,
    5. Matei A,
    6. Segonzac C,
    7. Malinovsky FG,
    8. Rathjen JP,
    9. MacLean D,
    10. Romeis T, et al.
    (2014) The calcium-dependent protein kinase CPK28 buffers plant immunity and regulates BIK1 turnover. Cell Host Microbe 16: 605–615
    OpenUrlCrossRefPubMed
  45. ↵
    1. Murata Y,
    2. Mori IC,
    3. Munemasa S
    (2015) Diverse stomatal signaling and the signal integration mechanism. Annu Rev Plant Biol 66: 369–392
    OpenUrlCrossRefPubMed
  46. ↵
    1. Noël L,
    2. Moores TL,
    3. van Der Biezen EA,
    4. Parniske M,
    5. Daniels MJ,
    6. Parker JE,
    7. Jones JD
    (1999) Pronounced intraspecific haplotype divergence at the RPP5 complex disease resistance locus of Arabidopsis. Plant Cell 11: 2099–2112
    OpenUrlAbstract/FREE Full Text
  47. ↵
    1. Nomura H,
    2. Komori T,
    3. Uemura S,
    4. Kanda Y,
    5. Shimotani K,
    6. Nakai K,
    7. Furuichi T,
    8. Takebayashi K,
    9. Sugimoto T,
    10. Sano S, et al.
    (2012) Chloroplast-mediated activation of plant immune signalling in Arabidopsis. Nat Commun 3: 926
    OpenUrlCrossRefPubMed
  48. ↵
    1. Obayashi T,
    2. Hayashi S,
    3. Saeki M,
    4. Ohta H,
    5. Kinoshita K
    (2009) ATTED-II provides coexpressed gene networks for Arabidopsis. Nucleic Acids Res 37: D987–D991
    OpenUrlCrossRefPubMed
  49. ↵
    1. Qudeimat E,
    2. Faltusz AM,
    3. Wheeler G,
    4. Lang D,
    5. Holtorf H,
    6. Brownlee C,
    7. Reski R,
    8. Frank W
    (2008) A PIIB-type Ca2+-ATPase is essential for stress adaptation in Physcomitrella patens. Proc Natl Acad Sci USA 105: 19555–19560
    OpenUrlAbstract/FREE Full Text
  50. ↵
    1. Rizo J,
    2. Südhof TC
    (1998) C2-domains, structure and function of a universal Ca2+-binding domain. J Biol Chem 273: 15879–15882
    OpenUrlFREE Full Text
  51. ↵
    1. Rossignol P,
    2. Collier S,
    3. Bush M,
    4. Shaw P,
    5. Doonan JH
    (2007) Arabidopsis POT1A interacts with TERT-V(I8), an N-terminal splicing variant of telomerase. J Cell Sci 120: 3678–3687
    OpenUrlAbstract/FREE Full Text
  52. ↵
    1. Schiøtt M,
    2. Romanowsky SM,
    3. Baekgaard L,
    4. Jakobsen MK,
    5. Palmgren MG,
    6. Harper JF
    (2004) A plant plasma membrane Ca2+ pump is required for normal pollen tube growth and fertilization. Proc Natl Acad Sci USA 101: 9502–9507
    OpenUrlAbstract/FREE Full Text
  53. ↵
    1. Schütze K,
    2. Harter K,
    3. Chaban C
    (2009) Bimolecular fluorescence complementation (BiFC) to study protein-protein interactions in living plant cells. Methods Mol Biol 479: 189–202
    OpenUrlCrossRefPubMed
  54. ↵
    1. Shigaki T,
    2. Hirschi KD
    (2006) Diverse functions and molecular properties emerging for CAX cation/H+ exchangers in plants. Plant Biol (Stuttg) 8: 419–429
    OpenUrlCrossRefPubMed
  55. ↵
    1. Steinhorst L,
    2. Kudla J
    (2013) Calcium and reactive oxygen species rule the waves of signaling. Plant Physiol 163: 471–485
    OpenUrlFREE Full Text
  56. ↵
    1. Sze H,
    2. Liang F,
    3. Hwang I,
    4. Curran AC,
    5. Harper JF
    (2000) Diversity and regulation of plant Ca2+ pumps: Insights from expression in yeast. Annu Rev Plant Physiol Plant Mol Biol 51: 433–462
    OpenUrlCrossRefPubMed
  57. ↵
    1. Thor K,
    2. Peiter E
    (2014) Cytosolic calcium signals elicited by the pathogen-associated molecular pattern flg22 in stomatal guard cells are of an oscillatory nature. New Phytol 204: 873–881
    OpenUrlCrossRefPubMed
  58. ↵
    1. Tidow H,
    2. Poulsen LR,
    3. Andreeva A,
    4. Knudsen M,
    5. Hein KL,
    6. Wiuf C,
    7. Palmgren MG,
    8. Nissen P
    (2012) A bimodular mechanism of calcium control in eukaryotes. Nature 491: 468–472
    OpenUrlCrossRefPubMed
  59. ↵
    1. Tomsig JL,
    2. Sohma H,
    3. Creutz CE
    (2004) Calcium-dependent regulation of tumour necrosis factor-alpha receptor signalling by copine. Biochem J 378: 1089–1094
    OpenUrlAbstract/FREE Full Text
  60. ↵
    1. Wang Y,
    2. Hua J
    (2009) A moderate decrease in temperature induces COR15a expression through the CBF signaling cascade and enhances freezing tolerance. Plant J 60: 340–349
    OpenUrlCrossRefPubMed
  61. ↵
    1. Whittaker CA,
    2. Hynes RO
    (2002) Distribution and evolution of von Willebrand/integrin A domains: Widely dispersed domains with roles in cell adhesion and elsewhere. Mol Biol Cell 13: 3369–3387
    OpenUrlAbstract/FREE Full Text
  62. ↵
    1. Wiermer M,
    2. Feys BJ,
    3. Parker JE
    (2005) Plant immunity: The EDS1 regulatory node. Curr Opin Plant Biol 8: 383–389
    OpenUrlCrossRefPubMed
  63. ↵
    1. Yang Y,
    2. Costa A,
    3. Leonhardt N,
    4. Siegel RS,
    5. Schroeder JI
    (2008) Isolation of a strong Arabidopsis guard cell promoter and its potential as a research tool. Plant Methods 4: 6
    OpenUrlCrossRefPubMed
  64. ↵
    1. Yang S,
    2. Hua J
    (2004) A haplotype-specific Resistance gene regulated by BONZAI1 mediates temperature-dependent growth control in Arabidopsis. Plant Cell 16: 1060–1071
    OpenUrlAbstract/FREE Full Text
  65. ↵
    1. Yang S,
    2. Yang H,
    3. Grisafi P,
    4. Sanchatjate S,
    5. Fink GR,
    6. Sun Q,
    7. Hua J
    (2006) The BON/CPN gene family represses cell death and promotes cell growth in Arabidopsis. Plant J 45: 166–179
    OpenUrlCrossRefPubMed
  66. ↵
    1. Zhai Z,
    2. Gayomba SR,
    3. Jung HI,
    4. Vimalakumari NK,
    5. Piñeros M,
    6. Craft E,
    7. Rutzke MA,
    8. Danku J,
    9. Lahner B,
    10. Punshon T, et al.
    (2014) OPT3 is a phloem-specific iron transporter that is essential for systemic iron signaling and redistribution of iron and cadmium in Arabidopsis. Plant Cell 26: 2249–2264
    OpenUrlAbstract/FREE Full Text
  67. ↵
    1. Zhu X,
    2. Caplan J,
    3. Mamillapalli P,
    4. Czymmek K,
    5. Dinesh-Kumar SP
    (2010) Function of endoplasmic reticulum calcium ATPase in innate immunity-mediated programmed cell death. EMBO J 29: 1007–1018
    OpenUrlAbstract/FREE Full Text
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Calcium Pumps and Interacting BON1 Protein Modulate Calcium Signature, Stomatal Closure, and Plant Immunity
Dong-Lei Yang, Zhenying Shi, Yongmei Bao, Jiapei Yan, Ziyuan Yang, Huiyun Yu, Yun Li, Mingyue Gou, Shu Wang, Baohong Zou, Dachao Xu, Zhiqi Ma, Jitae Kim, Jian Hua
Plant Physiology Sep 2017, 175 (1) 424-437; DOI: 10.1104/pp.17.00495

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Calcium Pumps and Interacting BON1 Protein Modulate Calcium Signature, Stomatal Closure, and Plant Immunity
Dong-Lei Yang, Zhenying Shi, Yongmei Bao, Jiapei Yan, Ziyuan Yang, Huiyun Yu, Yun Li, Mingyue Gou, Shu Wang, Baohong Zou, Dachao Xu, Zhiqi Ma, Jitae Kim, Jian Hua
Plant Physiology Sep 2017, 175 (1) 424-437; DOI: 10.1104/pp.17.00495
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Plant Physiology: 175 (1)
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SIGNALING AND RESPONSE

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