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Two SUMO Proteases SUMO PROTEASE RELATED TO FERTILITY1 and 2 Are Required for Fertility in Arabidopsis

Linpo Liu, Ying Jiang, Xiaomei Zhang, Xu Wang, Yanbing Wang, Yuzhen Han, George Coupland, Jing Bo Jin, Iain Searle, Yong-Fu Fu, Fulu Chen
Linpo Liu
MOA Key Laboratory of Soybean Biology (Beijing), National Key Facility of Crop Gene Resource and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 100081 Beijing, People’s Republic of ChinaCollege of Biological Sciences, State Key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, 100094 Beijing, People’s Republic of China
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Ying Jiang
MOA Key Laboratory of Soybean Biology (Beijing), National Key Facility of Crop Gene Resource and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 100081 Beijing, People’s Republic of ChinaCollege of Biological Sciences, State Key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, 100094 Beijing, People’s Republic of ChinaBeijing Agro-Biotechnology Research Center, Beijing Academy of Agricultural and Forestry Sciences, 100097 Beijing, People’s Republic of China
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Xiaomei Zhang
MOA Key Laboratory of Soybean Biology (Beijing), National Key Facility of Crop Gene Resource and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 100081 Beijing, People’s Republic of China
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Xu Wang
MOA Key Laboratory of Soybean Biology (Beijing), National Key Facility of Crop Gene Resource and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 100081 Beijing, People’s Republic of China
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Yanbing Wang
College of Life Sciences, Peking University, 100871 Beijing, People’s Republic of China
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Yuzhen Han
College of Biological Sciences, State Key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, 100094 Beijing, People’s Republic of China
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George Coupland
Max Planck Institute for Plant Breeding, D-50829 Cologne, Germany
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Jing Bo Jin
Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093 Beijing, People’s Republic of China
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Iain Searle
School of Biological Sciences, University of Adelaide-Shanghai Jiao Tong University Joint Centre for Agriculture and Health, University of Adelaide, Adelaide 5005, Australia
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Yong-Fu Fu
MOA Key Laboratory of Soybean Biology (Beijing), National Key Facility of Crop Gene Resource and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 100081 Beijing, People’s Republic of China
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  • For correspondence: fuyongfu@caas.cnchenfulu@caas.cn
Fulu Chen
MOA Key Laboratory of Soybean Biology (Beijing), National Key Facility of Crop Gene Resource and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 100081 Beijing, People’s Republic of China
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  • For correspondence: fuyongfu@caas.cnchenfulu@caas.cn

Published December 2017. DOI: https://doi.org/10.1104/pp.17.00021

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  • © 2017 American Society of Plant Biologists. All Rights Reserved.

Abstract

In plants, the posttranslational modification small ubiquitin-like modifier (SUMO) is involved in regulating several important developmental and cellular processes, including flowering time control and responses to biotic and abiotic stresses. Here, we report two proteases, SUMO PROTEASE RELATED TO FERTILITY1 (SPF1) and SPF2, that regulate male and female gamete and embryo development and remove SUMO from proteins in vitro and in vivo. spf1 mutants exhibit abnormal floral structures and embryo development, while spf2 mutants exhibit largely a wild-type phenotype. However, spf1 spf2 double mutants exhibit severe abnormalities in microgametogenesis, megagametogenesis, and embryo development, suggesting that the two genes are functionally redundant. Mutation of SPF1 and SPF2 genes also results in misexpression of generative- and embryo-specific genes. In vitro, SPF1 and SPF2 process SUMO1 precursors into a mature form, and as expected in vivo, spf1 and spf2 mutants accumulate SUMO conjugates. Using a yeast two-hybrid screen, we identified EMBRYO SAC DEVELOPMENT ARREST9 (EDA9) as an SPF1-interacting protein. In vivo, we demonstrate that EDA9 is sumolyated and that, in spf1 mutants, EDA9-SUMO conjugates increase in abundance, demonstrating that EDA9 is a substrate of SPF1. Together, our results demonstrate that SPF1 and SPF2 are two SUMO proteases important for plant development in Arabidopsis (Arabidopsis thaliana).

  • Received January 9, 2017.
  • Accepted October 22, 2017.
  • Published October 24, 2017.
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Two SUMO Proteases SUMO PROTEASE RELATED TO FERTILITY1 and 2 Are Required for Fertility in Arabidopsis
Linpo Liu, Ying Jiang, Xiaomei Zhang, Xu Wang, Yanbing Wang, Yuzhen Han, George Coupland, Jing Bo Jin, Iain Searle, Yong-Fu Fu, Fulu Chen
Plant Physiology Dec 2017, 175 (4) 1703-1719; DOI: 10.1104/pp.17.00021

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Two SUMO Proteases SUMO PROTEASE RELATED TO FERTILITY1 and 2 Are Required for Fertility in Arabidopsis
Linpo Liu, Ying Jiang, Xiaomei Zhang, Xu Wang, Yanbing Wang, Yuzhen Han, George Coupland, Jing Bo Jin, Iain Searle, Yong-Fu Fu, Fulu Chen
Plant Physiology Dec 2017, 175 (4) 1703-1719; DOI: 10.1104/pp.17.00021
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Plant Physiology: 175 (4)
Plant Physiology
Vol. 175, Issue 4
Dec 2017
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