Table of Contents
ON THE INSIDE
EDITORIAL
LETTER TO THE EDITOR
NEWS AND VIEWS
LETTERS
genome uncoupled1 Mutants Are Hypersensitive to Norflurazon and LincomycinArabidopsis gun1 mutants show hypersensitive phenotypes to both norflurazon and lincomycin.
The genomes uncoupled Mutants Are More Sensitive to Norflurazon Than Wild TypeThe increased sensitivity of the genomes uncoupled mutants to norflurazon indicates that they are probably deficient in plastid-to-nucleus signaling and are unequivocally not resistant to norflurazon.
BREAKTHROUGH TECHNOLOGIES
Engineering Artificial MicroRNAs for Multiplex Gene Silencing and Simplified Transgenic ScreenImprovements to amiRNA technology, including amiRNA design, transgenic screening for maximal gene-silencing mutants, and multiplex performance, facilitate its applications in plants.
Articles
ECOPHYSIOLOGY AND SUSTAINABILITY
An in Vivo Imaging Assay Detects Spatial Variability in Glucose Release from Plant RootsA gel-based, enzyme-coupled imaging assay demonstrates tightly regulated and species-specific spatial patterns of glucose release from roots.
BIOCHEMISTRY AND METABOLISM
The Patterned Structure of Galactoglucomannan Suggests It May Bind to Cellulose in Seed MucilageGalactoglucomannan synthesized by CSLA2 and MAGT1 has a patterned structure that may influence the mode of binding to cellulose fibrils.
Early Senescence in Older Leaves of Low Nitrate-Grown Atxdh1 Uncovers a Role for Purine Catabolism in N SupplyPurine catabolism mutants reveal a role for purine-related nitrogen sources in older leaves during low nitrate supply.
Identification of Key Enzymes for Pectin Synthesis in Seed MucilageMutations in two glycosyltransferase-encoding genes severely impair the elongation of pectic rhamnogalacturonan I, resulting in hydrophobic seeds that do not release mucilage polymers.
Differential Roles of the Thylakoid Lumenal Deg Protease Homologs in Chloroplast ProteostasisPhysiological, biochemical, and proteomic analyses of Arabidopsis mutants reveal the relationship between Deg1 and Deg5-Deg8 protease complexes and demonstrate a more prominent role in growth for Deg1.
Reconfigured Cyanogenic Glucoside Biosynthesis in Eucalyptus cladocalyx Involves a Cytochrome P450 CYP706C55Cyanogenic glucoside biosynthesis in Eucalyptus cladocalyx requires an additional pathway step, involving three CYPs from the CYP79, CYP706, and CYP71 families, and a glucosyltransferase UGT85.
Elucidation of the Amygdalin Pathway Reveals the Metabolic Basis of Bitter and Sweet Almonds (Prunus dulcis)Two missing enzyme activities in the amygdalan pathway result in sweet versus formation of bitter almonds.
CELL BIOLOGY
Chloroplast Damage Induced by the Inhibition of Fatty Acid Synthesis Triggers Autophagy in ChlamydomonasInhibition of fatty acid synthase in Chlamydomonas activates major catabolic pathways, including autophagy, through a mechanism that involves the activation of a chloroplast quality control system.
Actin Reorganization Triggers Rapid Cell Elongation in RootsCytokinin signaling promotes actin bundling in Arabidopsis root cells, which triggers rapid cell elongation.
Comparison of Micropore Distribution in Cell Walls of Softwood and Hardwood XylemMolecular microscopy shows that distribution of porosity across the secondary cell wall in xylem is predominantly related to the degree of lignification in different cell wall layers.
GENES, DEVELOPMENT AND EVOLUTION
Arabidopsis PIRL6 Is Essential for Male and Female Gametogenesis and Is Regulated by Alternative SplicingA leucine-rich repeat protein related to animal cell signaling components is important for gametogenesis, with gametophytespecific expression achieved by alternative mRNA splicing.
FLOWERING LOCUS T3 Controls Spikelet Initiation But Not Floral DevelopmentFLOWERING LOCUS T3 (FT3) promotes spikelet formation but not floral development under long- and short-day conditions in barley.
Timing and Order of the Molecular Events Marking the Onset of Berry Ripening in GrapevineA transcriptomic and metabolomic map of grape berry development elucidates timing and order of the key events of the global reprogramming featuring the ripening process.
A Trihelix Family Transcription Factor Is Associated with Key Genes in Mixed-Linkage Glucan AccumulationA trihelix family transcription factor regulates the expression of a mixed-linkage glucan biosynthesis gene and a gene encoding a MLG:xyloglucan endotransglucosylase.
Evidence for the Regulation of Gynoecium Morphogenesis by ETTIN via Cell Wall DynamicsETTIN, a transcription factor also known as Auxin Response Factor 3, enhances PME activity in the valve tissue, thus maintaining low cell wall stiffness and proper gynoecium morphogenesis.
INCREASING NODULE SIZE1 Expression Is Required for Normal Rhizobial Symbiosis and Nodule DevelopmentGmINS1, a candidate gene underlying a major QTL for nodulation, functions in nodule development and is important for nitrogen fixation in soybean.
Time-Course Transcriptome Analysis of Arabidopsis Siliques Discloses Genes Essential for Fruit Development and MaturationDynamic changes in the transcriptome of developing seedless fruits reveals key regulators of fruit growth and maturation.
MEMBRANES, TRANSPORT AND BIOENERGETICS
Nodulin Intrinsic Protein 7;1 Is a Tapetal Boric Acid Channel Involved in Pollen Cell Wall FormationNIP7;1 is a dicot-specific boric acid permease on the plasma membrane of tapetum cells, and is necessary for pollen exine wall formation during microsporogenesis under limiting boric acid conditions.
SIGNALING AND RESPONSE
MKK6 Functions in Two Parallel MAP Kinase Cascades in Immune SignalingArabidopsis MKK6 has multiple functions in plant defense responses in addition to its role in cytokinesis.
MdMYB88 and MdMYB124 Enhance Drought Tolerance by Modulating Root Vessels and Cell Walls in AppleMdMYB88 and MdMYB124 are critical for apple (Malus × domestica) tolerance to drought by mediating root architecture, xylem vessel development, and cell wall deposition.
Distinct Roles of Non-Overlapping Surface Regions of the Coiled-Coil Domain in the Potato Immune Receptor Rx1Mutation of the coiled-coil domain of the intracellular immune receptor Rx1 led to distinct cell death and resistance phenotypes and revealed regions of Rx1 required for interactions between Rx1 and the co-factor RanGAP2.
VAMP711 Is Required for Abscisic Acid-Mediated Inhibition of Plasma Membrane H+-ATPase ActivityThe SNARE protein VAMP711 is involved in regulating abscisic acid-mediated inhibition of plasma membrane H+-ATPase activity in response to drought stress.
Non-Enzymatic Synthesis of Bioactive Isoprostanoids in the Diatom Phaeodactylum following Oxidative StressThe non-enzymatic peroxidation of polyunsaturated fatty acids present in phosphatidylcholine and betaine lipids generates isoprostanoids that mediate part of the oxidative stress response.
Chloroplast Accumulation Response Enhances Leaf Photosynthesis and Plant Biomass ProductionChloroplast accumulation response facilitates efficient light capture in leaves and is required for the enhancement of leaf photosynthesis and plant biomass production.
Root Gravitropism Is Regulated by a Crosstalk between para-Aminobenzoic Acid, Ethylene, and AuxinEndogenous free para-aminobenzoic acid levels modulate the auxin-ethylene crosstalk necessary for root gravitropism
SENSITIVE TO SALT1, An Endoplasmic Reticulum-Localized Chaperone, Positively Regulates Salt ResistanceMolecular chaperone SES1 positively regulates salt stress resistance by alleviating salt-induced ER stress.
Digital Imaging Combined with Genome-Wide Association Mapping Links Loci to Plant-Pathogen Interaction TraitsComputer vision allows the identification of genes controlling uncharacterized lesion traits in Arabidopsis.
