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Review ArticleTOPICAL REVIEW
Open Access

Targeting Root Ion Uptake Kinetics to Increase Plant Productivity and Nutrient Use Efficiency

Marcus Griffiths, Larry M. York
Marcus Griffiths
Noble Research Institute, LLC, Ardmore, Oklahoma 73401
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Larry M. York
Noble Research Institute, LLC, Ardmore, Oklahoma 73401
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  • For correspondence: lmyork@noble.org

Published April 2020. DOI: https://doi.org/10.1104/pp.19.01496

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Abstract

Root system architecture has received increased attention in recent years; however, significant knowledge gaps remain for physiological phenes, or units of phenotype, that have been relatively less studied. Ion uptake kinetics studies have been invaluable in uncovering distinct nutrient uptake systems in plants with the use of Michaelis-Menten kinetic modeling. This review outlines the theoretical framework behind ion uptake kinetics, provides a meta-analysis for macronutrient uptake parameters, and proposes new strategies for using uptake kinetics parameters as selection criteria for breeding crops with improved resource acquisition capability. Presumably, variation in uptake kinetics is caused by variation in type and number of transporters, assimilation machinery, and anatomical features that can vary greatly within and among species. Critically, little is known about what determines transporter properties at the molecular level or how transporter properties scale to the entire root system. A meta-analysis of literature containing measures of crop nutrient uptake kinetics provides insights about the need for standardization of reporting, the differences among crop species, and the relationships among various uptake parameters and experimental conditions. Therefore, uptake kinetics parameters are proposed as promising target phenes that integrate several processes for functional phenomics and genetic analysis, which will lead to a greater understanding of this fundamental plant process. Exploiting this genetic and phenotypic variation has the potential to greatly advance breeding efforts for improved nutrient use efficiency in crops.

Sustainable crop production will be an ever-increasing challenge as the world population and global food demand continue to rise (Hunter et al., 2017). Improvements in food production need to be made despite threats from climate change and competition for land, water, and energy (Godfray et al., 2010; Parry and Hawkesford, 2010). A particular concern is the dependency of modern agriculture on chemical fertilizers that require finite fossil fuels and mineral reserves for their manufacture (Cordell et al., 2009). The world cannot be considered food secure until dependency on fertilizers is minimized. In order to mitigate these risks, the development of new cultivars that are more efficient in water and nutrient uptake is essential. During the first “Green Revolution”, above-ground phenes, or elemental units of phenotype (York et al., 2013), were the target of crop selection in breeding programs. Roots were largely ignored; however, indirect selection leading to changes in crop root systems over time may have occurred due to direct selection of above-ground phenes and yield in high-input and high plant-density farming systems (York and Lynch, 2015; Waines and Ehdaie, 2007). Selection of crops based on the root system is inherently challenging, but is predicted to pave the way for a second “Green Revolution” to help attain food security (Lynch, 2007).

Figure1
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The root system of a plant provides vital functions including resource uptake, storage, and anchorage in the soil, and is an interface between the plant and the soil microbiome. As soil resources are spatially and temporally heterogeneous, adaptations in the root system can be particularly important for survival (Wang et al., 2012). For plant growth and development, the elements nitrogen (N), sulfur (S), phosphorus (P), magnesium (Mg), calcium (Ca), and potassium (K) are required in the greatest amounts (Hawkesford, 2011). Of these macronutrients, N, P, and K are often at limiting quantities for crop yield in agriculture and are applied as fertilizers (Fageria, 2009). Chemical fertilizers are widely used to enrich soils and enhance crop productivity, but they also add a significant cost to food production and represent major environmental pollutants (Bumb and Baanante, 1996). Fertilizer utilization in agriculture is neither sustainable nor efficient, with as little as 50% of applied fertilizer being captured by the roots of crops (Pask et al., 2012). In addition, selection of crops in nonlimiting nutrient environments may have limited gains in nutrient acquisition efficiency. Hence, understanding the mechanisms involved in plant nutrient uptake is of key importance for improving worldwide agricultural production and mitigating environmental risks.

With the development of image-based root phenotyping, significant advances in characterizing root system architecture have been made in recent years (Bucksch et al., 2014; Atkinson et al., 2015; Colombi et al., 2015; Rellán-Álvarez et al., 2015; York and Lynch, 2015). However, whereas the importance of where roots are located and how they are arranged as determined by root system architecture is relatively well-known, functional processes such as root cortical senescence, root respiration, and root nutrient uptake have received much less attention. The advent of functional phenomics offers one path forward by combining high-throughput phenotyping, physiology, multivariate statistics, and simulation modeling (York, 2019). Nutrient uptake kinetics is the study of localized uptake rates of ions from external solutions by roots and the use of mathematical models to summarize uptake rate dependence on environmental and physiological conditions.

The aims of this review are to (1) describe the fundamentals of ion movement processes in the soil, the mechanisms involved with plant uptake of ions, and the popular mathematical models of nutrient uptake; (2) evaluate current experimental approaches used for ion uptake kinetics and provide a comprehensive meta-analysis of kinetics parameters reported in the literature across multiple crop species and nutrients; and (3) discuss future prospects for ion uptake studies in advancing plant science and the incorporation of findings in crop breeding programs.

THEORETICAL FRAMEWORK

Processes of Ion Movement in the Soil Environment

Root uptake of mineral nutrients from the soil solution is of major importance for plant growth. Nutrients in soil must be intercepted by the root surface through either nutrient movement or root growth to be taken up by the plant. Plants themselves also influence the physical structure of the soil and resource availability by root-driven soil displacement and increased porosity and the release of root exudates (Tisdall and Oades, 1982; Helliwell et al., 2017). Solute movement and availability at the root surface are mediated by the processes of mass flow and diffusion (Barber, 1962). Mass flow is the process of an ion moving across a water potential gradient which occurs while the plant is transpiring. Nitrate (NO3−) is an example of an ion with high solubility, and it therefore has greater uptake at peak plant transpiration (Le Bot and Kirkby, 1992). The solubility of nitrate also affects its soil availability, as nitrate typically rapidly leaches into the deeper soil layers and ground water, is lost through surface run off, or is temporarily immobilized during drought (Bray, 1954). When mass flow does not saturate the root uptake capacity, ions will be depleted from the surrounding soil environment. This depletion from the soil causes a concentration gradient in which ions move from a high concentration to a lower concentration passively by diffusion. The effective diffusion rate in the soil is influenced by multiple parameters, including the electrochemical gradient and charge of the nutrient, the ion-exchange capacity of the soil, and the moisture content (York, Carminati, et al., 2016). Phosphate is relatively immobile in soil for this reason, and therefore, small depletion zones form around roots. Despite phosphate being relatively immobile, it also contributes to eutrophication of lakes, rivers, and marine environments via attachment to soil particles during erosion. Soil nutrient mobility and availability play a large part in determining the optimal root system architecture, but little is known about how optimal uptake kinetics are determined by nutrient mobility.

Physical Mechanisms of Plant Ion Uptake

The soil environment to which a root is exposed can vary greatly within a field, where nutrient concentrations are often lower than the internal concentrations of the root cells (Barber, 1995; Lark et al., 2004). Therefore, plants have evolved mechanisms to passively facilitate and regulate ion transport down favorable electrochemical gradients as well as actively move ions against these gradients. Primary active transport uses ATP to move a substrate across a membrane against its gradient, whereas secondary active transport utilizes a gradient previously established by primary active transport. For both nutrient anions and cations, secondary active transport across the plasma membrane is mediated by H+-ATPases that establish electrical and proton gradients that drive uptake (Reid and Hayes, 2003).

Plant genomes encode many types of membrane-bound transport proteins that are important for nutrient uptake and mobilization in the plant (Zelazny and Vert, 2014). Transporters often exhibit specificity to particular nutrients in their most common chemical forms. This specificity is important for a plant to preferentially absorb ions that are in demand and to block those that are either not needed or toxic. Individual nutrient chemical species are known to have subtypes of transporters responsible for their uptake with unique functional properties and regulatory patterns (O’Brien et al., 2016). For instance, a high-affinity transport system and a low-affinity transport system for nitrate have transporter proteins encoded by the NPF (also known as NRT1 and PTR) and NRT2 gene families, respectively, in Arabidopsis (Arabidopsis thaliana; Tsay et al., 2007). Whereas these genes are most commonly studied in Arabidopsis, analogs in cereals are known. In rice (Oryza sativa), OsNPF6.5 (NRT1.1B) was found to be sustainably induced by nitrate and showed dual-affinity nitrate transport (Hu et al., 2015). In maize (Zea mays), the analogous ZmNrt1 and ZmNrt2 genes correspond to differences in uptake relating to expression levels (Quaggiotti et al., 2003, 2004; Trevisan et al., 2008). Although nitrate is generally regarded as the nutrient most commonly limiting growth, other macronutrients are also of interest, especially in nutrient-poor soils. Transporters have been identified for ammonium (Howitt and Udvardi, 2000; Sohlenkamp et al., 2000), phosphate (Raghothama, 2000), potassium (Coskun et al., 2013), and sulfate (Takahashi et al., 2012). Adding to the complexity, transporters have been demonstrated to exhibit cross regulation with multiple nutrients at the tissue and whole-plant levels as well as to facilitate uptake of phytohormones (Krouk et al., 2010; Medici et al., 2019). The genetics and transcriptional regulation of transporters have been identified in many cases but our understanding of the physical mechanisms as to how ions are intercepted and shuttled across the membrane by individual transporters is relatively poor.

Little is known about transporter-level uptake kinetics or how it scales up to root segments or whole-root systems (discussed in York et al., 2016). This knowledge gap is exacerbated because of the primitive understanding of the physical and molecular mechanisms involved in how an ion approaches the cell membrane, is bound by a transporter, and then is shuttled by the transporter changing conformations from the external to the internal environment (Fig. 1). Recent research has supported proton-coupled transport of nitrate by NPF6.3 (also known as CHL1 and NRT1.1) and an alternating access mechanism where a central binding site reorients to alternatively expose the bound nitrate from the external to the internal solution (Parker and Newstead, 2014). When NPF6.3 is phosphorylated, the nitrate affinity becomes greater, yet maximum uptake rate is decreased (Parker and Newstead, 2014; Sun et al., 2014). The fact that both the maximum uptake rate and the affinity can be posttranslationally modified in a single transporter type is a strong indicator that the specific molecular form of a transporter influences kinetics. It follows that there may be different alleles within a species or homologs among species that have different transporter properties that influence uptake capability. Understanding the scaling of transporter-level uptake to the whole-root system must begin with a deeper understanding of these molecular foundations of individual transporters, yet even less is known about how transporters and other molecular machinery behave in groups and combinations.

Figure 1.
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Figure 1.

The scaling from the soil environment to transporter-level dynamics to harness the potential of root uptake kinetics. A, Soil nutrient mobility and bioavailability determine interception of the root surfaces with nutrient ions. B, Ions may travel across the root via the symplastic pathway through cells or the apoplastic pathway around cells to reach the xylem for transport throughout the plant (Heymans et al., 2020). C, Nutrient ions may enter the root from the soil solution through a variety of mechanisms, as depicted. D, Whereas general mechanisms are known, the state of knowledge is too limited to address questions about how transporter-level properties may be influenced by genetic variation and how these properties can be utilized to breed or engineer more nutrient acquisition-efficient crops.

The numbers and types of transporters found in the root epidermis must logically scale to root segment-level uptake. A linear scaling was showing for transporter abundance with uptake for the prokaryotic chloride channel (ClC) transporter (Garcia-Celma et al., 2013). However, to our knowledge, no study has quantified the numbers of specific nutrient transporters in a given root surface area, i.e. transporter density. How the types of transporters and their density modulate scaling of transporter-level dynamics to the root level is not known. Whereas several studies have shown a relationship between transporter transcript abundance and uptake rates at single external concentrations (Quaggiotti et al., 2004; Trevisan et al., 2008; Garnett et al., 2015), they typically do not quantify Imax and Km, or the maximal uptake rate and half-saturation constant, respectively. Those studies also have not revealed the relationship between transcript abundance and transporter density at the root surface. Therefore, how uptake kinetics scale from transporter to root segment remains a knowledge gap. A complete physical mechanistic model at the molecular and cellular level is highly desirable as a foundation for understanding these dynamics, yet modeling of uptake kinetics in plants has generally been accomplished using Michaelis-Menten kinetics at the root-segment or whole root-system level.

The activity of many nutrient transporters is linked to the establishment of hydrogen ion gradients using ATPase to pump H+ outside of the cell (Fig. 1C). For example, the phosphate and nitrate transporters are symporters where the anion nutrient and a proton together move into the cell. Like transporters, little is known about how the numbers or types of ATPase proteins influence the electrochemical gradient or uptake rate. For iron acquisition, concerted action among ATPases, reductases, and iron transporters is required, which highlights the importance of spatial distribution of these proteins (Gayomba et al., 2015). Therefore, one of the most crucial conceptual gaps to address before utilizing nutrient uptake to increase plant productivity is how the specific types and numbers of transporters, ATPase proteins, and assimilation machinery influence uptake at the root segment and whole root system levels (Fig. 1D; also discussed conceptually in York et al. [2016] and more formally in Le Deunff et al. [2019]).

Michaelis-Menten Kinetics

The active uptake of nutrients by plants is most often modeled using the Michaelis-Menten theoretical framework of enzyme-substrate saturation kinetics (see Equation 1; Fig. 2). Epstein and Hagen (1952) first used Michaelis-Menten kinetics to model uptake of nutrients by roots. Using this framework, Epstein et al. (1963) uncovered two distinct root uptake systems for potassium in barley (Hordeum vulgare) that operate at low and high concentration ranges (>250 μm and <250 μm). Since then, there have been numerous studies identifying distinct uptake systems for other ions in multiple plant species (Siddiqi et al., 1990; Rao et al., 1997). In Michaelis-Menten formulation, as the external concentration of a solute (C) increases, the chance of binding to the respective transporter protein also increases, resulting in a greater net uptake rate or influx (In). When this external concentration continues to increase, a plateau is reached with a maximal uptake rate (Imax) as the carrier binding sites are saturated. The affinity between the carrier and ion is represented by the Km, which is the half saturation constant where the solute concentration is 1/2Imax. Less reported is the minimum substrate concentration at which net influx can occur (Cmin).

Figure 2.
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Figure 2.

Example of an ion uptake kinetics graph showing the relationship between external ion concentration and ion influx rate. The curve and highlighted derived parameters are calculated using nonlinear least squares to fit the Michaelis-Menten model.

The Michaelis-Menten kinetic model is described by Equation 1.Embedded Image(1)

Alternatives to Michaelis-Menten Modeling

Use of Michaelis-Menten modeling for uptake by root segments or whole-root systems presupposes that the behavior of the entire system of multiple transporters, assimilation, and uptake into vacuoles can be aggregated by a single mathematical function. However, this assumption limits our understanding of what may cause variation in uptake kinetics that could be used for breeding because the formalism does not include transporter-level properties, scaling, or accounting for cellular external and internal concentrations. Alternatives to the enzyme-substrate model for nutrient uptake include the porter-diffusion model, which has more flexibility at the cellular level, and the flow-force interpretation, which is more realistic at the macroscopic scale (Le Deunff et al., 2019).

The porter-diffusion model framework was developed for modeling nutrient uptake by aquatic phytoplankton (for review, see Shaw et al., 2015). The original work coupled the Michaelis-Menten model with the concept of a diffusion boundary surrounding cells to accurately predict nutrient concentration at the cell surface containing the transporters (Pasciak and Gavis, 1974). Over time, this porter-diffusion modeling framework has led to various refinements that allow additional flexibility with incorporation of cell and transporter characteristics such as cell size, number of transporters, transporter area, and substrate handling time by transporters, as well as physical properties such as diffusion and convection (Dugdale, 1967; Aksnes and Egge, 1991; Armstrong, 2008; Aksnes and Cao, 2011). Combined with Michaelis-Menten formalism, a porter-diffusion model can be scaled between a cell and colonies of cells for phytoplankton modeling (Fiksen et al., 2013). Because the porter-diffusion framework is more mechanistic at the molecular and cellular levels, it may be an ideal starting place for incorporation into plant models and for guiding empirical research. As an example, the following equation from Aksnes and Egge (1991) determines finite uptake rate (V) as a function of the number of transporters (n), the “ion-catch” area (A), ion handling time (h), external nutrient concentration (S), and the mass transfer coefficient or velocity (v).

The porter-diffusion model with transporter-scale parameters is described by Equation 2.Embedded Image(2)

At the macroscopic scale, the flow-force model first proposed by Thellier (1970) operates at the whole root-system level for ion flux and root conductance (see Equation 3). Flow-force differs from Michaelis-Menten kinetics in that it is based on thermodynamic absorption of ions rather than enzyme activity. Therefore, flow-force modeling disregards transporter assumptions and does not allow deduction of plant transporter affinities along ion concentration ranges (Le Deunff and Malagoli, 2014). The flow-force framework is promising because it describes the macroscopic conductance of the whole root for the substrate and introduces more flexibility to use for physiological plasticity, root aging, or differences among root classes and positions along roots (Le Deunff et al., 2019). Flow-force models may be suited for scaling from root systems to cropping systems by including total uptake at a given concentration (Jj), root conductance (Lj; based on thermodynamic considerations), the external concentration (Embedded Image), and the external concentration at which no uptake occurs (Embedded Image).

The flow-force model is described by Equation 3.Embedded Image(3)

Models inherently have limitations and therefore should be interpreted with consideration. Being able to summarize and link nutrient uptake to physiological phenes and transporter regulation is challenging because these occur at different spatial and temporal scales. A combination of porter-diffusion modeling and flow-force modeling is a proposition that could enable the development of new models that retain flexibility at different biological scales (Le Deunff et al., 2019). This spatial and temporal aspect has been explored with nutrient and water uptake across differential root tissue types and developmental zones (Foster and Miklavcic, 2014; Sakurai et al., 2015); however, these models do not include active transport, which is the focus of this review. Other key improvements would be to include multiple ions and transporters, incorporate electrochemical gradients, and model how multiple ions compete or facilitate uptake. At present, an operable and simple nutrient uptake model that incorporates spatial and temporal scales is still lacking.

EXPERIMENTAL APPROACHES FOR STUDYING ROOT ION UPTAKE KINETICS

Many experimental approaches have been employed to study ion uptake kinetics in roots, but most are hydroponics-based and so lack the physical and chemical properties of soil. Ion uptake studies generally quantify the net influx, which is the difference between gross influx and efflux of a particular ion into the root from an external solution (BassiriRad, 2000). Measurements of uptake kinetics have spanned from root segments to the whole-root system. A full kinetics assessment would provide Imax, Km, and Cmin values; however, measuring the influx rate for a single external concentration and time point can provide a snapshot of the plant performance at a particular concentration. Singular influx rate experiments are more easily scaled to large populations and allow the comparison of more experimental conditions. The uptake kinetic capabilities of a plant are highly dependent on the growth and measurement environments. Differences in laboratory protocols for growing plants and in uptake assay methods may explain the variability of Imax, Km, and Cmin values in the literature. In this section, ion uptake kinetic protocols and variables in experimental setups are discussed.

Depletion versus Accumulation

Experimental setups for ion uptake kinetics fall into two main categories, specifically measurement of ion depletion from a nutrient solution and measurement of ion accumulation in the plant, with the latter method employing either stable isotopes, such as 15N, or radiotracers, such as 32P. In general, the depletion-based method is convenient because no radioactive material is used and only standard analytical chemistry instruments are needed. In addition, depletion studies are nondestructive, because rather than sampling all plant tissue, as in accumulation studies, only the solution is sampled, which enables the study of temporal ion uptake kinetics for characterizing the same plants across multiple plant ages and plasticity responses to nutrient availability. However, depletion studies using nonlabeled nutrients cannot distinguish influx from efflux and therefore only allow quantification of net influx. Techniques for quantifying ion depletion of a solution include ion chromatography, colorimetric assays, and ion selective electrodes. However, stable isotopes and radiotracers are useful when available, as short accumulation measures can be made at both very low and high nutrient concentrations (Lee and Drew, 1986). Labeled nutrients are the only method for determining absolute influx and can be used for tracking accumulation through the plant. In addition, they are convenient for determining ion uptake rates in the field and foraging capacity at depth (Kristensen and Thorup-Kristensen, 2004). Promising technologies such as microdialysis and ion selective electrodes, which measures ion concentrations from the soil solution based on passive diffusion, offer further opportunities for ion kinetic depletion studies in soil without the use of tracers; however, at present, these approaches are low throughput (Hawkins et al., 2008; Shabala et al., 2013; Oyewole et al., 2014; Shaw et al., 2014).

Depletion over Concentration Ranges versus Depletion over Time

Plants have different mechanisms for uptake across nutrient concentration ranges. Passive diffusion occurs across the cell membrane when nutrients are found in high external concentrations. When nutrients are scarce, ATP-mediated active transport is likely to occur, and therefore measured ion influx rates will reflect the plant capabilities in that environment. For example, nitrate has three main transport systems: a constitutive and an inducible high-affinity transport system, both characterized by low Km and Imax values (Km = 6–20 μm and 20–100 μm, respectively), and a constitutive low-affinity transport system, which dominates transport activity and uptake at concentrations >250 μm and fails to saturate at concentrations as high as 50 mm (Crawford and Glass, 1998; Ho et al., 2009; Hu et al., 2009). Therefore, the sampling strategy is important to consider in plant ion influx experiments, as the ion concentrations tested provide information on the respective transport mechanisms and their working ranges.

There are two main depletion-based methods for estimating kinetic parameters for nutrient uptake. The first method will be referred to as “depletion over concentration ranges” and relies on characterizing nutrient uptake of different individual plants placed in different nutrient concentrations that represent the range of relevant concentrations (originally in Epstein and Hagen, 1952). The second method will be referred to as “depletion over time” and relies on measuring nutrient uptake by a single plant starting at a high nutrient concentration and measuring depletion from the same solution over time at set intervals (originally in Claassen and Barber, 1974).

The depletion-over-concentration-ranges experimental design is advantageous in that it allows a broad external concentration range to be tested in a short time (5–60 min). However, this entails the use of multiple plants and vessels and therefore introduces interplant variability. By contrast, for depletion-over-time experiments, the concentration range tested is typically narrower and starts at a lower concentration (<100 μm) to allow for ion depletion within hours to avoid diurnal effects. In order to characterize a plant across a wide nutrient concentration range while minimizing the number of experimental plants, these methods could be combined. Depletion over time can be used to calculate depletion rates at lower concentrations that can be depleted to Cmin within a few hours, and depletion over concentration ranges can provide rates at concentrations too high for full depletion but that allow for accurate measurement of Imax.

Intact versus Excised Roots

Preparation of root samples for ion uptake kinetics varies among studies, with either intact or excised roots being used. Using intact root systems minimizes disruption to the plant and so, presumably, provides more representative ion uptake kinetic measurements. Once a root is excised, the carbon and nutrient balance maintained in the root system is irreversibly altered, with carbohydrate exhaustion of root tissues, falling respiration rates, and nutrient movement through the plant disrupted (Farrar, 1985; BassiriRad et al., 1999; Jones et al., 2005). Using excised roots is often necessary; however, for field-grown plants, excavation of intact plants from soil is challenging, and thus excision studies should be conducted as quickly as possible to avoid disruption to uptake processes (BassiriRad et al., 1999).

Excised root studies have been valuable for in-depth analysis of ion influx along root zones, classes, and ages. Rao et al. (1997) demonstrated that there is variation in nitrate uptake rates along the maize primary root. In another study, Sorgonà et al. (2011) showed that there is spatial and temporal heterogeneity in nitrate uptake along maize root axes, with a significantly lower Imax in apical compared to basal regions but no detectable difference when fully induced. More recently, individual root segment-specific chambers were used to demonstrate variation in Imax and Km among root classes in maize without excising the roots by using short PVC chambers sealed around root segments (York et al., 2016). These segment-level measures are valuable, because the uptake kinetics measured when using the intact entire root system are aggregated across root positions and classes, whereas research has shown that uptake kinetics vary along roots of a particular class (i.e. seminal) and also among classes (i.e. seminal, nodal, or lateral). However, since lateral roots dominate the root system by length, root system-level uptake kinetics may largely reflect lateral-root uptake kinetics. These new advances greatly improve the validity of the kinetic parameters reported, and therefore, root excision should be avoided where possible due to possible wound responses (Gronewald and Hanson, 1980).

Deprivation and Induction

The expression and activity of ion transport proteins in plants are highly dependent on the environment and plant nutrient status. Depriving a plant of a focal nutrient and then exposing it to a high nutrient concentration for a period of time has been widely reported to substantially increase plant influx rates. For example, nitrate influx rates were shown to be significantly greater in N-starved plants compared to replete plants (Siddiqi et al., 1990; Raman et al., 1995). In addition, influx rates can be further increased by a nitrate induction period after deprivation (Lee and Drew, 1986; Hole et al., 1990). Therefore, deprivation and induction steps are commonly used in kinetic studies for determining the genetic potential of a genotype with measurements conducted when the plant is operating at near-maximum uptake capacities.

A key question is whether deprivation and induction steps provide representative ion uptake rates for kinetics experiments compared to soil. These steps directly affect plant nutrient balance and demand, which in turn affects ion transporter expression, and therefore should be considered as critical when using uptake rates as selection criteria for plant breeding. The effects of induction and deprivation steps on ion uptake rates are concentration, time, and species dependent, and whether plants experience such conditions in the field has not been explored (Maeck and Tischner, 1986; Siddiqi et al., 1989). Deprivation and induction steps can eliminate plant plasticity responses, and the uptake rates measured may not be representative of those in the field. These experimental manipulations are used because they maximize Imax and may represent the maximum potential uptake rate for a plant species or variety. In the case of deprivation-only studies, where no nutrient is supplied, it is possible that internal cellular concentrations decline substantially and the concentration gradient drives much of the observed increased uptake. Future research should address the relevance of these methodologies.

Meta-Analysis

The most recent meta-analysis of nutrient kinetics dates to Raman et al. (1995) for nitrate. They found that literature values for Km varied more than those for Imax, speculating that this may reflect both the sensitivity of Km estimates to measurement artifacts and an inherent variability due to the complexity of the uptake process. In addition, they implied that the Km values were relatively low, so nitrate can be reduced to very low concentrations by plants. Here, a new meta-analysis is presented for uptake kinetics across multiple crop species and for multiple nutrient types: nitrate, phosphate, and potassium. The meta-analysis data and statistical analysis code are available (https://doi.org/10.5281/zenodo.3605654).

To summarize the current state of crop ion uptake kinetic research, maize is the most widely characterized crop for ion uptake kinetics, with approximately half of all studies focusing on maize; however, there are also a substantial number of studies for barley and rice (Fig. 3A). By comparison, wheat (Triticum aestivum), sorghum (Sorghum bicolor), soybean (Glycine max), and rapeseed (Brassica napus) combined account for only 8 of the 50 collated studies. The nutrients investigated in these studies were distributed among nitrate, phosphate, and potassium, with the greatest number of studies (20 of 50) focused on nitrate. Two-thirds of ion uptake studies were with seedlings 0–21 d old, and the oldest specified plant age was 60 d in rice (Fig. 3B; Teo et al., 1992). Most studies used intact root systems, with one-quarter of studies using excised root samples (Fig. 3C). For the plant pretreatment process, there was no clear preferred method, with deprivation-only and no-pretreatment conditions the most widely used, in 21 and 20 studies, respectively (Fig. 3D).

Figure 3.
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Figure 3.

Summary of ion uptake kinetics studies for nitrate, phosphate, and potassium in major crops. A, Study count by plant species and nutrient of interest. B, Plant age distribution (days). C, Root sample type used for ion uptake rate determination. D, Plant pretreatment process used before ion uptake rate determination.

With the collated meta-analysis data for ion uptake kinetics, global trends can be observed. Results are given for nitrate, because other nutrient data were relatively sparse. At the species level, Figure 4E shows the variation for nitrate Imax, with maize and rice having greater uptake rates compared to wheat and barley. For Km, there were no substantial differences among species and high variability (Fig. 4F). The influences of experimental conditions upon kinetics parameters were also determined. Interestingly, Imax weakly correlated with the influx rate at 50 μm, demonstrating the importance of maximum uptake capability in determining uptake even at low concentrations (Fig. 4A). However, Imax was not correlated to Km, which supports Michaelis-Menten formalism, as compared to some porter-diffusion models that indicate a possible dependency of these parameters (Fig. 4B). In addition, the ranking of studies by highest uptake rate strongly predicted Imax rank, which supports the use of single uptake rates at high concentrations to approximate relative Imax for high-throughput phenotyping of mapping populations, since full uptake curves would be more difficult to achieve at that scale. However, analyzing influx rates across nutrient ranges is important for determining the exact Imax saturation point, and Km could not be predicted from other easier-to-measure values, such as uptake at low concentrations. Another benefit of determining ion influx rates across nutrient ranges is that Michaelis-Menten curves have wider utility for comparison to other studies that use similar concentration ranges. Considering the inconvenience of converting literature kinetics parameters to a common standard, the uptake rate on a root mass basis (specific uptake rate in micromoles per gram dry weight per hour) should be reported along with on root length and volume bases.

Figure 4.
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Figure 4.

Meta-analysis of kinetic parameters for nitrate uptake in different crop species. Data are for barley (●), wheat (▲), rice (▪), and maize (+). A to D, Relationship between Imax and Km (A), the uptake rate at 50 μm (B), the highest uptake rate reported (C), and the highest concentration used in the respective study (D). E and F, Nitrate Imax (E) and Km variability (F) by species.

Ion uptake kinetics research so far illustrates that there is substantial genetic and phenotypic variation in plants that could be used to improve nutrient uptake efficiency. However, there are very few studies comparing genotypic variation within the same species (Supplemental S1). The greatest number of genotypes compared is 16, occurring twice in studies for maize and rice (Pace and McClure, 1986; Hasegawa and Ichii, 1994). These studies illustrate that there is variation for ion uptake phenes among cultivars within a species. In addition, to our knowledge, there has been only a single study reporting multiple ion uptake parameters across multiple genotypes (Baligar and Barber, 1979). In that study, potassium, phosphate, calcium, and magnesium Imax and Km were determined from the excised roots of 12 maize genotypes. Single-ion studies are more abundant, but to date there are still no mapping population-sized studies for single-ion or multiple-ion uptake kinetics. A key difficulty is that experimental approaches currently available are both challenging and time-consuming for high-throughput phenotyping of ion uptake.

FUTURE PROSPECTS

Uptake Kinetics for Advancing Plant Research

This review reported on the current state of research on ion uptake kinetics by roots, outlining the background concepts, describing fundamental methods, and providing a comprehensive meta-analysis of parameters reported in the literature. Ion uptake kinetics have not been sufficiently studied in plant research and significant knowledge gaps remain regarding the genetic, physical, and molecular mechanisms involved in nutrient uptake by roots (see Outstanding Questions ).

In general, the study of nutrient uptake has focused on a single nutrient at a time, often from solution containing only that nutrient, while ignoring the interplay among substrates. Whereas this approach is useful for a fundamental understanding of the uptake kinetics for a specific nutrient, it complicates the translation of experimental results to real-world conditions, where soil solutions generally contain a complex complement of nutrients in varying concentrations. Since electrochemical gradients influence plant nutrient uptake, the uptake of a nutrient is influenced by other nutrients with the same, as well as opposite, charges (Cox and Reisenauer, 1973). Although it is most relevant under field conditions, this critical area of nutrient uptake kinetic interactions is understudied.

In addition to characterizing ion transporters, ion uptake kinetic studies have been valuable in determining the function and contribution of physiological phenes and environmental factors that relate to ion uptake. Factors that influence ion uptake kinetics include plant species, cultivars, plant developmental age, root class, and position along a root (Jungk and Barber, 1975; Baligar and Barber, 1979; Rao et al., 1997; BassiriRad et al., 1999; Garnett et al., 2013; Sorgonà et al., 2011;York et al., 2016), in addition to environmental conditions such as external ion concentration, nutrient distribution, temperature, and pH (Carter and Lathwell, 1967; Claassen and Barber, 1974; Rao and Rains, 1976; Kochian and Lucas, 1982). However, there are many unexplored phenes that could significantly affect ion uptake rates and uptake efficiencies, such as electrochemical gradient establishment, root system architecture, root anatomy, root respiration efficiency, photosynthesis, and transpiration (Fig. 5). An additional unexplored area of complexity is potential variation among genotypes for transporter types, transporter densities, and assimilation processes that may drive uptake by developing a chemical gradient. Characterization of ion transporter properties may explain the variation in Imax observed among species and illustrate why Km varies less among species (Fig. 4, E and F). In general, Imax is supposed to scale positively with increased transporter density, whereas Km may not be influenced. Conceptualizing and modeling the optimal uptake configuration necessitates more information about the relative benefits and costs of transporter abundance, types, maintaining electrochemical gradients, and assimilation, as well as the interactions among these parameters.

Figure 5.
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Figure 5.

The aggregative hierarchy for uptake kinetics showing how underlying phenes create the emergent property measured as uptake kinetics. Nutrient uptake is determined by the integration of underlying properties, and it ultimately influences nutrient use efficiency and yield.

Whereas the benefits of more and faster transporters seem obvious, knowledge gaps surrounding the costs of synthesis, maintenance, and activity of these transporters need to be filled to determine an optimal uptake configuration. Synthesis costs depend on the protein size and the energy required for transcription, translation, and mobilization to the membrane. Synthesis and maintenance costs are also opportunity costs because maintaining a maximum uptake rate configuration when soil nutrient concentrations are low and unable to saturate the uptake machinery may not allow the plant to invest in other processes such as root growth instead. Activity costs during uptake are largely due to the synthesis of ATP for maintaining electrochemical gradients with the H+ ATPase pumps (Sze et al., 1999). In maize, 20% of total plant respiration was estimated to be devoted to nitrate uptake (Veen, 1980). In barley, 5% of root respiration was determined to be devoted to nitrate absorption and 15% to assimilation (Bloom et al., 1992). Maintaining a uniformly high Imax capacity throughout an entire root system regardless of soil nutrient levels may be extremely costly and inefficient.

Nutrient concentrations in soils are heterogeneously located in time and space (Dunbabin et al., 2004) due to fertilizer and residue placement. Therefore, plants may routinely construct roots with less uptake capacity that are capable of increasing uptake capacity when a patch of high nutrient concentration is encountered, which could be a cost-saving strategy. The plasticity in maximum uptake rate is often termed physiological plasticity in the literature, as opposed to morphological plasticity, or proliferation of roots in patches (Hodge, 2004). Simulation studies have shown that ion uptake plasticity may be especially important for exploiting patches of nitrate and has a positive but smaller effect for phosphate, which is less mobile (Jackson and Caldwell, 1996). In studies that placed roots from the same root system in either high phosphate or distilled water prior to measurements in solution containing phosphate, phosphate uptake was found to be almost double for the roots previously exposed to the high phosphate (Jackson et al., 1990). This may explain why root tips are often found to have greater Imax, because as roots deplete soil nutrients and grow into new regions, less uptake capacity is required in the mature root zones remaining. In fact, the deprivation and induction methods used for kinetics assays actually use uptake-rate plasticity to maximize uptake rates. Plasticity is likely governed not by transporter type or constitutive abundance, but rather by the capability of the plant to differentially transcribe and translate transporter genes or other molecular machinery and then locate them to the epidermal membrane. Ion uptake plasticity may be under independent genetic control with the transcript levels of transporter genes responding to nutrient supply dependent on crop genotype (El-Kereamy et al., 2011; Garnett et al., 2015). Further research is needed on how to measure ion uptake plasticity, its genetic diversity, and its functional ramifications in various soils.

Ion kinetics research is key for parameterization of holistic root models including OpenSimRoot (Postma et al., 2017), ROOTMAP (Diggle, 1988; Dunbabin et al., 2003), CRootBox (Mai et al., 2018), and R-SWMS (Javaux et al., 2008). Incorporation of ion kinetics data for more crops, root types, and developmental stages would greatly improve the validity and representativeness of the models. Using such models that simulate plant growth and environmental conditions dynamically can help to compare the relative importance of kinetic parameters in a variety of simulated environments. In addition, a theoretical ideotype could be devised by simulating all combinations of ion kinetic parameters and root architectural phenes in a variety of nutrient-limiting and nutrient-replete environments. The value of this approach was demonstrated in a previous study (York et al., 2016), where nitrate uptake parameters from maize were used to parameterize the holistic model SimRoot (now called OpenSimRoot). By factorially combining kinetic parameters and root system architectural phenes, additive effects were observed between beneficial nitrate kinetics states stacked with favorable root architectural phene states to improve plant performance. Through the use of simulations, Dunbabin et al. (2004) also found that the benefits of increased uptake capacity depended on theoretical highly branched (herringbone) or sparsely branched (dichotomous) root systems. Parametrization with a greater number of species and populations would be valuable in determining strategies and ideotypes in a variety of environments and management scenarios. In addition, modeling of root plasticity responses is currently limited, with little research on the responses of Imax and Km to nonuniform resource supply.

Incorporation of Ion Uptake Kinetics into Crop Prebreeding Programs

Ion uptake kinetics studies have been invaluable in uncovering distinct nutrient uptake systems in plants and have the potential to greatly advance breeding efforts. However, prebreeding activities will be required to better understand the underlying physiology and genetics, and to transfer novel traits into breeding lines. As illustrated in the meta-analysis, there is substantial variation in uptake parameters among species and cultivars, making them a viable target for breeding efforts (Fig. 4). To our knowledge, the most complete documentation of intraspecific variation is in the work by Pace and McClure (1986), who found a 2.3-fold variation in Imax and a 12.8-fold variation in Km among maize inbred lines. The potential for variation is also supported by work that shows uptake variation along roots and among root classes (such as York et al., 2016). Whereas different transporter gene families for the same nutrient have been discovered, potential allelic variation for uptake kinetics has rarely been considered. One notable example that demonstrates direct utility of uptake kinetics is a report of allelic variation in a nitrate transporter and differential uptake capability between two subspecies of rice (Hu et al., 2015). Together, this research demonstrates that there is genetic and phenotypic potential available that may be harnessed for plant breeding.

The kinetics of ion uptake by roots is very difficult to measure, especially in field conditions. Potential aboveground proxies, such as leaf nutrient content or nutrient use efficiency, could accelerate plant breeding if tightly coupled to uptake kinetics. A common agronomic definition for nutrient use efficiency described by Moll et al. (1982) is the ratio of yield (or biomass) to fertilizer input (or nutrient availability in soil). This agronomic use efficiency can be subcategorized into nutrient uptake efficiency, specifically the ratio of nutrient in biomass to nutrient availability, and nutrient utilization efficiency, specifically the ratio of yield to nutrient in biomass. Utilization efficiency is determined by many processes that are not directly related to root function, such as photosynthetic capability and nutrient remobilization. Uptake efficiency is generally taken to be largely determined by root processes. Root system architecture determines the overall exploration and potential for local exploitation of soil resources. Specific uptake rates or kinetics parameters would also influence uptake efficiency and use efficiency, but these relationships become more indirect because of the interaction of many phenes (York et al., 2013). Similarly, multiple-ion uptake is partly addressed by the study of the plant ionome, meaning the contents and concentrations of elements (Salt, 2004). Ionomics has uncovered numerous genetic components for both macro- and micronutrient accumulation (Baxter et al., 2008, 2010; Segura et al., 2012; Pinson et al., 2015; Yang et al., 2018). Components linking uptake with accumulated tissue content have been shown in maize for nitrate (Dechorgnat et al., 2018); however, due to the influence of many interacting processes, shoot nutrient content may not be a reliable proxy for ion uptake kinetics by roots and needs further research.

It is suggested that nutrient uptake efficiency explains most of the variation in nutrient use efficiency and genetic progress in yield, so root traits are viable breeding targets (Hirel et al., 2007; Li et al., 2015; Nehe et al., 2018). Identified loci that associate with uptake, assimilation, biomass, and grain yield simultaneously will be especially important for breeding higher-yielding and nutrient-efficient crops (Yang et al., 2018). Even though breeding has historically been based on yield- or biomass-based crop selection, comparing historic varieties with modern varieties of wheat indicated that selection for yield also indirectly selected for higher total nitrogen uptake over total root length with smaller root systems (Aziz et al., 2017), which could be due to both more efficient soil exploration and specific nitrogen uptake capacity. In most studies, nutrient use efficiency and nutrient content will be completely correlated because the nutrient supply is the same for all genotypes tested. For example, Li et al. (2015) found only moderate correlations among root system architectural traits and nitrogen content in maize under high and low nitrogen supply, and even those relationships may be due to plant vigor and allometry. Including nutrient uptake kinetics in such analyses may improve the explanatory power of the statistical models. Based upon a previous observation that a less-vigorous wheat genotype acquired as much nitrogen as more vigorous genotypes in the field, Pang et al. (2015) determined that the less-vigorous genotype had greater Imax and lower Km for nitrate. These results illustrate that an understanding of and breeding for ion uptake kinetics is possible.

Ideotype, or trait-based, breeding supposes that understanding and selecting phenes known to influence plant physiology can accelerate gains relative to selection on yield alone (Donald, 1968). Ideotypes have been proposed for root architecture and these could be further improved by utilizing uptake parameters (Lynch, 2013; Zhan and Lynch, 2015; Morris et al., 2017). A crop ideotype with respect to uptake kinetics would be a plant that has a high Imax, low Km, and low Cmin, depending on the associated costs, possible trade-offs, and the respective nutrient soil mobility and availability. High Imax would be particularly important for high-input agricultural systems or mobile nutrients when the rate of mass flow is sufficient to supply the respective nutrient in great enough concentrations to sustain the Imax uptake rate. For low-input agricultural systems, soils susceptible to nutrient leaching, or immobile nutrients, breeding for a low Km and Cmin should take priority, as it would allow greater uptake when external ion concentrations are below that needed to attain Imax, and it also increases the pool of nutrients available to the plant for net uptake. Model sensitivity analysis by Silberbush and Barber (1983) indicated that increased Imax would not benefit plant growth in low-phosphate conditions due to its relative immobility, and this was partially confirmed in work that overexpressed phosphate transporters in barley with no effect on uptake (Rae et al., 2004). Ion uptake kinetic parameters change greatly across the plant lifecycle, and this will also have to be carefully considered in crop selection so that uptake characteristics are beneficial in that particular environment (Jungk and Barber, 1975; Garnett et al., 2013). Additionally, ion uptake parameters can be used to select species for multiple-species systems such as cover crops, where ecological niches exhibit complementarity and reduce intraspecific competition within monocultures (Wendling et al., 2017).

Figure7
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The greatest technological challenges for ion uptake kinetics as a breeding target are developing high-throughput phenotyping for mapping populations and verifying uptake capacity in soil. Using an automated hydroponic platform with chamber-specific control of nutrient concentrations and a sensor system for logging ion depletion across time, ion uptake kinetics could be scaled up to population-sized studies. A genome-wide association mapping study would for the first time provide a significant genetic insight into ion uptake kinetics. Identification of genes and pathways involved in uptake kinetics could allow selection of crops with improved uptake efficiency. Not only would known transporter genes be confirmed, but new allelic variations and completely novel components with important contributions to the molecular machinery of ion uptake could be discovered while simultaneously addressing knowledge gaps. In the long term, knowledge gained about uptake capacity will have to be integrated with knowledge of soil microbes, soil properties, exudations, and soil water content in order to understand the holistic rhizosphere (York, Carminati, et al., 2016). Confirmation of uptake capacity in soil would require using techniques like excavation of living roots or microdialysis. Considering ion uptake kinetics parameters or specific uptake rates as breeding targets provides an exciting opportunity to combine basic plant biological research with applied agronomy and breeding in order to design sustainable agroecosystems to address food insecurity.

Supplemental Data

The supplemental materials are available at https://doi.org/10.5281/zenodo.3605654.

Footnotes

  • M.G. and L.Y. conceived the article; M.G. collated and analyzed data for the meta-analysis, produced figures, and wrote the first draft; M.G. and L.Y. revised the final version and approved it for submission.

  • www.plantphysiol.org/cgi/doi/10.1104/pp.19.01496

  • ↵1 This work was supported by the USDA/National Institute of Food and Agriculture (NIFA; grant no. 2017-67007-25948), Noble Research Institute, LLC. and the Center for Bioenergy Innovation, a U.S. Department of Energy (DOE) Bioenergy Research Center supported by the Biological and Environmental Research in the DOE Office of Science.

  • ↵3 Senior author.

  • ↵[OPEN] Articles can be viewed without a subscription.

  • Received December 5, 2019.
  • Accepted January 20, 2020.
  • Published February 6, 2020.

REFERENCES

  1. ↵
    1. Aksnes D,
    2. Cao F
    (2011) Inherent and apparent traits in microbial nutrient uptake. Mar Ecol Prog Ser 440: 41–51
    OpenUrlCrossRef
  2. ↵
    1. Aksnes D,
    2. Egge J
    (1991) A theoretical model for nutrient uptake in phytoplankton. Mar Ecol Prog Ser 70: 65–72
    OpenUrlCrossRef
  3. ↵
    1. Armstrong RA
    (2008) Nutrient uptake rate as a function of cell size and surface transporter density: A Michaelis-like approximation to the model of Pasciak and Gavis. Deep Sea Res Part I Oceanogr Res Pap 55: 1311–1317
    OpenUrl
  4. ↵
    1. Atkinson JA,
    2. Wingen LU,
    3. Griffiths M,
    4. Pound MP,
    5. Gaju O,
    6. Foulkes MJ,
    7. Le Gouis J,
    8. Griffiths S,
    9. Bennett MJ,
    10. King J, et al.
    (2015) Phenotyping pipeline reveals major seedling root growth QTL in hexaploid wheat. J Exp Bot 66: 2283–2292
    OpenUrlCrossRefPubMed
  5. ↵
    1. Aziz MM,
    2. Palta JA,
    3. Siddique KHM,
    4. Sadras VO
    (2017) Five decades of selection for yield reduced root length density and increased nitrogen uptake per unit root length in Australian wheat varieties. Plant Soil 413: 181–192
    OpenUrl
  6. ↵
    1. Baligar VC,
    2. Barber SA
    (1979) Genotypic differences of corn for ion uptake. Agron J 71: 870–873
    OpenUrl
  7. ↵
    1. Barber SA
    (1962) A diffusion and mass-flow concept of soil nutrient availability. Soil Sci 93: 39–49
    OpenUrlCrossRef
  8. ↵
    1. Barber SA
    (1995) Soil nutrient bioavailability: a mechanistic approach, 2nd ed. Wiley, New York
  9. ↵
    1. BassiriRad H
    (2000) Kinetics of nutrient uptake by roots: Responses to global change. New Phytol 147: 155–169
    OpenUrlCrossRef
  10. ↵
    1. BassiriRad H,
    2. Prior SA,
    3. Norby RJ,
    4. Rogers HH
    (1999) A field method of determining NH4+ and NO3− uptake kinetics in intact roots: Effects of CO2 enrichment on trees and crop species. Plant Soil 217: 195–204
    OpenUrlCrossRef
  11. ↵
    1. Baxter I,
    2. Brazelton JN,
    3. Yu D,
    4. Huang YS,
    5. Lahner B,
    6. Yakubova E,
    7. Li Y,
    8. Bergelson J,
    9. Borevitz JO,
    10. Nordborg M,
    11. Vitek O,
    12. Salt DE
    (2010) A coastal cline in sodium accumulation in Arabidopsis thaliana is driven by natural variation of the sodium transporter AtHKT1;1. PLoS Genet 6: e1001193
    OpenUrlCrossRefPubMed
  12. ↵
    1. Baxter I,
    2. Muthukumar B,
    3. Park HC,
    4. Buchner P,
    5. Lahner B,
    6. Danku J,
    7. Zhao K,
    8. Lee J,
    9. Hawkesford MJ,
    10. Guerinot ML, et al.
    (2008) Variation in molybdenum content across broadly distributed populations of Arabidopsis thaliana is controlled by a mitochondrial molybdenum transporter (MOT1). PLoS Genet 4: e1000004
    OpenUrlCrossRefPubMed
  13. ↵
    1. Bloom AJ,
    2. Sukrapanna SS,
    3. Warner RL
    (1992) Root respiration associated with ammonium and nitrate absorption and assimilation by barley. Plant Physiol 99: 1294–1301
    OpenUrlAbstract/FREE Full Text
  14. ↵
    1. Bray RH
    (1954) A nutrient mobility concept of soil-plant relationships. Soil Sci 78: 9–22
    OpenUrlCrossRef
  15. ↵
    1. Bucksch A,
    2. Burridge J,
    3. York LM,
    4. Das A,
    5. Nord E,
    6. Weitz JS,
    7. Lynch JP
    (2014) Image-based high-throughput field phenotyping of crop roots. Plant Physiol 166: 470–486
    OpenUrlAbstract/FREE Full Text
  16. ↵
    1. Bumb BL,
    2. Baanante CA
    (1996) The Role of Fertilizer in Sustaining Food Security and Protecting the Environment to 2020. International Food Policy Research Institute, Washington, D.C.
  17. ↵
    1. Carter OG,
    2. Lathwell DJ
    (1967) Effects of temperature on orthophosphate absorption by excised corn roots. Plant Physiol 42: 1407–1412
    OpenUrlAbstract/FREE Full Text
  18. ↵
    1. Claassen N,
    2. Barber SA
    (1974) A method for characterizing the relation between nutrient concentration and flux into roots of intact plants. Plant Physiol 54: 564–568
    OpenUrlAbstract/FREE Full Text
  19. ↵
    1. Colombi T,
    2. Kirchgessner N,
    3. Le Marié CA,
    4. York LM,
    5. Lynch JP,
    6. Hund A
    (2015) Next generation shovelomics: Set up a tent and REST. Plant Soil 388: 1–20
    OpenUrlCrossRef
  20. ↵
    1. Cordell D,
    2. Drangert J-O,
    3. White S
    (2009) The story of phosphorus: Global food security and food for thought. Glob Environ Change 19: 292–305
    OpenUrl
  21. ↵
    1. Coskun D,
    2. Britto DT,
    3. Li M,
    4. Oh S,
    5. Kronzucker HJ
    (2013) Capacity and plasticity of potassium channels and high-affinity transporters in roots of barley and Arabidopsis. Plant Physiol 162: 496–511
    OpenUrlAbstract/FREE Full Text
  22. ↵
    1. Cox WJ,
    2. Reisenauer HM
    (1973) Growth and ion uptake by wheat supplied nitrogen as nitrate, or ammonium, or both. Plant Soil 38: 363–380
    OpenUrlCrossRef
  23. ↵
    1. Crawford NM,
    2. Glass AD
    (1998) Molecular and physiological aspects of nitrate uptake in plants. Trends Plant Sci 3: 389–395
    OpenUrlCrossRef
  24. ↵
    1. Dechorgnat J,
    2. Francis KL,
    3. Dhugga KS,
    4. Rafalski JA,
    5. Tyerman SD,
    6. Kaiser BN
    (2018) Root ideotype influences nitrogen transport and assimilation in maize. Front Plant Sci 9: 531
    OpenUrl
  25. ↵
    1. Diggle AJ
    (1988) ROOTMAP—A model in three-dimensional coordinates of the growth and structure of fibrous root systems. Plant Soil 105: 169–178
    OpenUrlCrossRef
  26. ↵
    1. Donald CM
    (1968) The breeding of crop ideotypes. Euphytica 17: 385–403
    OpenUrl
  27. ↵
    1. Dugdale RC
    (1967) Nutrient limitation in the sea: Dynamics, identification, and significance. Limnol Oceanogr 12: 685–695
    OpenUrl
  28. ↵
    1. Dunbabin V,
    2. Diggle A,
    3. Rengel Z
    (2003) Is there an optimal root architecture for nitrate capture in leaching environments? Plant Cell Environ 26: 835–844
    OpenUrlCrossRefPubMed
  29. ↵
    1. Dunbabin V,
    2. Rengel Z,
    3. Diggle AJ
    (2004) Simulating form and function of root systems: Efficiency of nitrate uptake is dependent on root system architecture and spatial and temporal variability of nitrate supply. Funct Ecol 18: 204–211
    OpenUrl
  30. ↵
    1. El-Kereamy A,
    2. Guevara D,
    3. Bi YM,
    4. Chen X,
    5. Rothstein SJ
    (2011) Exploring the molecular and metabolic factors contributing to the adaptation of maize seedlings to nitrate limitation. Front Plant Sci 2: 49
    OpenUrlPubMed
  31. ↵
    1. Epstein E,
    2. Hagen CE
    (1952) A kinetic study of the absorption of alkali cations by barley roots. Plant Physiol 27: 457–474
    OpenUrlFREE Full Text
  32. ↵
    1. Epstein E,
    2. Rains DW,
    3. Elzam OE
    (1963) Resolution of dual mechanisms of potassium absorption by barley roots. Proc Natl Acad Sci USA 49: 684–692
    OpenUrlFREE Full Text
  33. ↵
    1. Fageria NK
    (2009) The Use of Nutrients in Crop Plants. CRC Press, Boca Raton, FL
  34. ↵
    1. Farrar JF
    (1985) Fluxes of carbon in roots of barley plants. New Phytol 99: 57–69
    OpenUrlCrossRef
  35. ↵
    1. Fiksen Ø,
    2. Follows MJ,
    3. Aksnes DL
    (2013) Trait-based models of nutrient uptake in microbes extend the Michaelis-Menten framework. Limnol Oceanogr 58: 193–202
    OpenUrl
  36. ↵
    1. Foster KJ,
    2. Miklavcic SJ
    (2014) On the competitive uptake and transport of ions through differentiated root tissues. J Theor Biol 340: 1–10
    OpenUrl
  37. ↵
    1. Garcia-Celma J,
    2. Szydelko A,
    3. Dutzler R
    (2013) Functional characterization of a ClC transporter by solid-supported membrane electrophysiology. J Gen Physiol 141: 479–491
    OpenUrlAbstract/FREE Full Text
  38. ↵
    1. Garnett T,
    2. Conn V,
    3. Plett D,
    4. Conn S,
    5. Zanghellini J,
    6. Mackenzie N,
    7. Enju A,
    8. Francis K,
    9. Holtham L,
    10. Roessner U, et al.
    (2013) The response of the maize nitrate transport system to nitrogen demand and supply across the lifecycle. New Phytol 198: 82–94
    OpenUrlCrossRefPubMed
  39. ↵
    1. Garnett T,
    2. Plett D,
    3. Conn V,
    4. Conn S,
    5. Rabie H,
    6. Rafalski JA,
    7. Dhugga K,
    8. Tester MA,
    9. Kaiser BN
    (2015) Variation for N uptake system in maize: genotypic response to N supply. Front Plant Sci 6: 936
    OpenUrl
  40. ↵
    1. Gayomba SR,
    2. Zhai Z,
    3. Jung HI,
    4. Vatamaniuk OK
    (2015) Local and systemic signaling of iron status and its interactions with homeostasis of other essential elements. Front Plant Sci 6: 716
    OpenUrl
  41. ↵
    1. Godfray HCJ,
    2. Beddington JR,
    3. Crute IR,
    4. Haddad L,
    5. Lawrence D,
    6. Muir JF,
    7. Pretty J,
    8. Robinson S,
    9. Thomas SM,
    10. Toulmin C
    (2010) Food security: The challenge of feeding 9 billion people. Science 327: 812–818
    OpenUrlAbstract/FREE Full Text
  42. ↵
    1. Gronewald JW,
    2. Hanson JB
    (1980) Sensitivity of the proton and ion transport mechanisms of corn roots to injury. Plant Sci Lett 18: 143–150
    OpenUrlCrossRef
  43. ↵
    1. Hasegawa H,
    2. Ichii M
    (1994) Variation in Michaelis-Menten kinetic parameters for nitrate uptake by the young seedlings in rice (Oryza sativa L.). Jpn J Breed 44: 383–386
    OpenUrl
  44. ↵
    1. P Barraclough, and
    2. MJ Hawkesford
    1. Hawkesford MJ
    (2011) An overview of nutrient use efficiency and strategies for crop improvement. In P Barraclough, and MJ Hawkesford, eds, The Molecular and Physiological Basis of Nutrient Use Efficiency in Crops. Wiley-Blackwell, Oxford, pp 5–19
  45. ↵
    1. Hawkins BJ,
    2. Boukcim H,
    3. Plassard C
    (2008) A comparison of ammonium, nitrate and proton net fluxes along seedling roots of Douglas-fir and lodgepole pine grown and measured with different inorganic nitrogen sources. Plant Cell Environ 31: 278–287
    OpenUrlCrossRefPubMed
  46. ↵
    1. Heymans A,
    2. Couvreur V,
    3. LaRue T,
    4. Paez-Garcia A,
    5. Lobet G
    (2020) GRANAR, a computational tool to better understand the functional importance of monocotyledon root anatomy. Plant Physiol 182: 707–720
    OpenUrlAbstract/FREE Full Text
  47. ↵
    1. Helliwell JR,
    2. Sturrock CJ,
    3. Mairhofer S,
    4. Craigon J,
    5. Ashton RW,
    6. Miller AJ,
    7. Whalley WR,
    8. Mooney SJ
    (2017) The emergent rhizosphere: Imaging the development of the porous architecture at the root-soil interface. Sci Rep 7: 14875
    OpenUrl
  48. ↵
    1. Hirel B,
    2. Le Gouis J,
    3. Ney B,
    4. Gallais A
    (2007) The challenge of improving nitrogen use efficiency in crop plants: Towards a more central role for genetic variability and quantitative genetics within integrated approaches. J Exp Bot 58: 2369–2387
    OpenUrlCrossRefPubMed
  49. ↵
    1. Ho C-H,
    2. Lin S-H,
    3. Hu H-C,
    4. Tsay Y-F
    (2009) CHL1 functions as a nitrate sensor in plants. Cell 138: 1184–1194
    OpenUrlCrossRefPubMed
  50. ↵
    1. Hodge A
    (2004) The plastic plant: Root responses to heterogeneous supplies of nutrients. New Phytol 162: 9–24
    OpenUrlCrossRef
  51. ↵
    1. Hole DJ,
    2. Emran AM,
    3. Fares Y,
    4. Drew MC
    (1990) Induction of nitrate transport in maize roots, and kinetics of influx, measured with nitrogen-13. Plant Physiol 93: 642–647
    OpenUrlAbstract/FREE Full Text
  52. ↵
    1. Howitt SM,
    2. Udvardi MK
    (2000) Structure, function and regulation of ammonium transporters in plants. Biochim Biophys Acta 1465: 152–170
    OpenUrlCrossRefPubMed
  53. ↵
    1. Hu B,
    2. Wang W,
    3. Ou S,
    4. Tang J,
    5. Li H,
    6. Che R,
    7. Zhang Z,
    8. Chai X,
    9. Wang H,
    10. Wang Y, et al.
    (2015) Variation in NRT1.1B contributes to nitrate-use divergence between rice subspecies. Nat Genet 47: 834–838
    OpenUrlCrossRefPubMed
  54. ↵
    1. Hu H-C,
    2. Wang Y-Y,
    3. Tsay Y-F
    (2009) AtCIPK8, a CBL-interacting protein kinase, regulates the low-affinity phase of the primary nitrate response. Plant J 57: 264–278
    OpenUrlCrossRefPubMed
  55. ↵
    1. Hunter MC,
    2. Smith RG,
    3. Schipanski ME,
    4. Atwood LW,
    5. Mortensen DA
    (2017) Agriculture in 2050: Recalibrating targets for sustainable intensification. Bioscience 67: 386–391
    OpenUrl
  56. ↵
    1. Jackson RB,
    2. Caldwell MM
    (1996) Integrating resource heterogeneity and plant plasticity: Modelling nitrate and phosphate uptake in a patchy soil environment. J Ecol 84: 891–903
    OpenUrlCrossRef
  57. ↵
    1. Jackson RB,
    2. Manwaring JH,
    3. Caldwell MM
    (1990) Rapid physiological adjustment of roots to localized soil enrichment. Nature 344: 58–60
    OpenUrlCrossRefPubMed
  58. ↵
    1. Javaux M,
    2. Schröder T,
    3. Vanderborght J,
    4. Vereecken H
    (2008) Use of a three-dimensional detailed modeling approach for predicting root water uptake. Vadose Zone J 7: 1079–1088
    OpenUrlAbstract/FREE Full Text
  59. ↵
    1. Jones DL,
    2. Healey JR,
    3. Willett VB,
    4. Farrar JF,
    5. Hodge A
    (2005) Dissolved organic nitrogen uptake by plants—An important N uptake pathway? Soil Biol Biochem 37: 413–423
    OpenUrlCrossRef
  60. ↵
    1. Jungk A,
    2. Barber SA
    (1975) Plant age and the phosphorus uptake characteristics of trimmed and untrimmed corn root systems. Plant Soil 42: 227–239
    OpenUrl
  61. ↵
    1. Kochian LV,
    2. Lucas WJ
    (1982) Potassium transport in corn roots: I. Resolution of kinetics into a saturable and linear component. Plant Physiol 70: 1723–1731
    OpenUrlAbstract/FREE Full Text
  62. ↵
    1. Kristensen HL,
    2. Thorup-Kristensen K
    (2004) Root growth and nitrate uptake of three different catch crops in deep soil layers. Soil Sci Soc Am J 68: 529–537
    OpenUrlCrossRef
  63. ↵
    1. Krouk G,
    2. Lacombe B,
    3. Bielach A,
    4. Perrine-Walker F,
    5. Malinska K,
    6. Mounier E,
    7. Hoyerova K,
    8. Tillard P,
    9. Leon S,
    10. Ljung K, et al.
    (2010) Nitrate-regulated auxin transport by NRT1.1 defines a mechanism for nutrient sensing in plants. Dev Cell 18: 927–937
    OpenUrlCrossRefPubMed
  64. ↵
    1. Lark RM,
    2. Milne AE,
    3. Addiscott TM,
    4. Goulding KWT,
    5. Webster CP,
    6. O’Flaherty S
    (2004) Scale- and location-dependent correlation of nitrous oxide emissions with soil properties: An analysis using wavelets. Eur J Soil Sci 55: 611–627
    OpenUrlCrossRef
  65. ↵
    1. Le Bot J,
    2. Kirkby EA
    (1992) Diurnal uptake of nitrate and potassium during the vegetative growth of tomato plants. J Plant Nutr 15: 247–264
    OpenUrlCrossRef
  66. ↵
    1. Le Deunff E,
    2. Malagoli P
    (2014) An updated model for nitrate uptake modelling in plants. I. Functional component: Cross-combination of flow-force interpretation of nitrate uptake isotherms, and environmental and in planta regulation of nitrate influx. Ann Bot 113: 991–1005
    OpenUrlCrossRefPubMed
  67. ↵
    1. Le Deunff E,
    2. Malagoli P,
    3. Decau M-L
    (2019) Modelling nitrogen uptake in plants and phytoplankton: Advantages of integrating flexibility into the spatial and temporal dynamics of nitrate absorption. Agronomy (Basel) 9: 116
    OpenUrl
  68. ↵
    1. Lee RB,
    2. Drew MC
    (1986) Nitrogen-13 studies of nitrate fluxes in barley roots. J Exp Bot 37: 1768–1779
    OpenUrlCrossRef
  69. ↵
    1. Li P,
    2. Chen F,
    3. Cai H,
    4. Liu J,
    5. Pan Q,
    6. Liu Z,
    7. Gu R,
    8. Mi G,
    9. Zhang F,
    10. Yuan L
    (2015) A genetic relationship between nitrogen use efficiency and seedling root traits in maize as revealed by QTL analysis. J Exp Bot 66: 3175–3188
    OpenUrlCrossRefPubMed
  70. ↵
    1. Lynch JP
    (2007) Roots of the second Green Revolution. Aust J Bot 55: 493–512
    OpenUrlCrossRef
  71. ↵
    1. Lynch JP
    (2013) Steep, cheap and deep: an ideotype to optimize water and N acquisition by maize root systems. Ann Bot 112: 347–357
    OpenUrlCrossRefPubMed
  72. ↵
    1. H Lambers,
    2. JJ Neeteson, and
    3. I Stulen
    1. Maeck G,
    2. Tischner R
    (1986) Nitrate uptake and reduction in sugarbeet seedlings. In H Lambers, JJ Neeteson, and I Stulen, eds, Fundamental, Ecological and Agricultural Aspects of Nitrogen Metabolism in Higher Plants. Springer, Dordrecht, pp 33–36
  73. ↵
    1. Mai TH,
    2. Schnepf A,
    3. Vereecken H,
    4. Vanderborght J
    (2018) Continuum multiscale model of root water and nutrient uptake from soil with explicit consideration of the 3D root architecture and the rhizosphere gradients. Plant Soil 439: 273–292
    OpenUrl
  74. ↵
    1. Medici A,
    2. Szponarski W,
    3. Dangeville P,
    4. Safi A,
    5. Dissanayake IM,
    6. Saenchai C,
    7. Emanuel A,
    8. Rubio V,
    9. Lacombe B,
    10. Ruffel S, et al.
    (2019) Identification of molecular integrators shows that nitrogen actively controls the phosphate starvation response in plants. Plant Cell 31: 1171–1184
    OpenUrlAbstract/FREE Full Text
  75. ↵
    1. Moll RH,
    2. Kamprath EJ,
    3. Jackson WA
    (1982) Analysis and interpretation of factors which contribute to efficiency of nitrogen utilization. Agron J 74: 562–564
    OpenUrlCrossRef
  76. ↵
    1. Morris EC,
    2. Griffiths M,
    3. Golebiowska A,
    4. Mairhofer S,
    5. Burr-Hersey J,
    6. Goh T,
    7. von Wangenheim D,
    8. Atkinson B,
    9. Sturrock CJ,
    10. Lynch JP, et al.
    (2017) Shaping 3D root system architecture. Curr Biol 27: R919–R930
    OpenUrlCrossRefPubMed
  77. ↵
    1. Nehe AS,
    2. Misra S,
    3. Murchie EH,
    4. Chinnathambi K,
    5. Foulkes MJ
    (2018) Genetic variation in N-use efficiency and associated traits in Indian wheat cultivars. Field Crops Res 225: 152–162
    OpenUrl
  78. ↵
    1. O’Brien JA,
    2. Vega A,
    3. Bouguyon E,
    4. Krouk G,
    5. Gojon A,
    6. Coruzzi G,
    7. Gutiérrez RA
    (2016) Nitrate transport, sensing, and responses in plants. Mol Plant 9: 837–856
    OpenUrlCrossRefPubMed
  79. ↵
    1. Oyewole OA,
    2. Inselsbacher E,
    3. Näsholm T
    (2014) Direct estimation of mass flow and diffusion of nitrogen compounds in solution and soil. New Phytol 201: 1056–1064
    OpenUrl
  80. ↵
    1. Pace GM,
    2. McClure PR
    (1986) Comparison of nitrate uptake kinetic parameters across maize inbred lines. J Plant Nutr 9: 1095–1111
    OpenUrlCrossRef
  81. ↵
    1. Pang J,
    2. Milroy SP,
    3. Rebetzke GJ,
    4. Palta JA
    (2015) The influence of shoot and root size on nitrogen uptake in wheat is affected by nitrate affinity in the roots during early growth. Funct Plant Biol 42: 1179–1189
    OpenUrl
  82. ↵
    1. Parker JL,
    2. Newstead S
    (2014) Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1. Nature 507: 68–72
    OpenUrlCrossRefPubMed
  83. ↵
    1. Parry MAJ,
    2. Hawkesford MJ
    (2010) Food security: Increasing yield and improving resource use efficiency. Proc Nutr Soc 69: 592–600
    OpenUrlCrossRefPubMed
  84. ↵
    1. Pask AJD,
    2. Sylvester-Bradley R,
    3. Jamieson PD,
    4. Foulkes MJ
    (2012) Quantifying how winter wheat crops accumulate and use nitrogen reserves during growth. Field Crops Res 126: 104–118
    OpenUrl
  85. ↵
    1. Pasciak WJ,
    2. Gavis J
    (1974) Transport limitation of nutrient uptake in phytoplankton. Limnol Oceanogr 19: 881–888
    OpenUrl
  86. ↵
    1. Pinson SRM,
    2. Tarpley L,
    3. Yan WG,
    4. Yeater K,
    5. Lahner B,
    6. Yakubova E,
    7. Huang XY,
    8. Zhang M,
    9. Guerinot ML,
    10. Salt DE
    (2015) Worldwide genetic diversity for mineral element concentrations in rice grain. Crop Sci 55: 294–331
    OpenUrlCrossRef
  87. ↵
    1. Postma JA,
    2. Kuppe C,
    3. Owen MR,
    4. Mellor N,
    5. Griffiths M,
    6. Bennett MJ,
    7. Lynch JP,
    8. Watt M
    (2017) OpenSimRoot: Widening the scope and application of root architectural models. New Phytol 215: 1274–1286
    OpenUrl
  88. ↵
    1. Quaggiotti S,
    2. Ruperti B,
    3. Borsa P,
    4. Destro T,
    5. Malagoli M
    (2003) Expression of a putative high-affinity NO3− transporter and of an H+-ATPase in relation to whole plant nitrate transport physiology in two maize genotypes differently responsive to low nitrogen availability. J Exp Bot 54: 1023–1031
    OpenUrlCrossRefPubMed
  89. ↵
    1. Quaggiotti S,
    2. Ruperti B,
    3. Pizzeghello D,
    4. Francioso O,
    5. Tugnoli V,
    6. Nardi S
    (2004) Effect of low molecular size humic substances on nitrate uptake and expression of genes involved in nitrate transport in maize (Zea mays L.). J Exp Bot 55: 803–813
    OpenUrlCrossRefPubMed
  90. ↵
    1. Rae AL,
    2. Jarmey JM,
    3. Mudge SR,
    4. Smith FW
    (2004) Over-expression of a high-affinity phosphate transporter in transgenic barley plants does not enhance phosphate uptake rates. Funct Plant Biol 31: 141–148
    OpenUrlCrossRef
  91. ↵
    1. Raghothama KG
    (2000) Phosphate transport and signaling. Curr Opin Plant Biol 3: 182–187
    OpenUrlCrossRefPubMed
  92. ↵
    1. Raman DR,
    2. Spanswick RM,
    3. Walker LP
    (1995) The kinetics of nitrate uptake from flowing solutions by rice: Influence of pretreatment and light. Bioresour Technol 53: 125–132
    OpenUrlCrossRef
  93. ↵
    1. Rao TP,
    2. Ito O,
    3. Matsunaga R,
    4. Yoneyama T
    (1997) Kinetics of 15 N-labelled nitrate uptake by maize (Zea mays L.) root segments. Soil Sci Plant Nutr 43: 491–498
    OpenUrl
  94. ↵
    1. Rao KP,
    2. Rains DW
    (1976) Nitrate absorption by barley: I. Kinetics and energetics. Plant Physiol 57: 55–58
    OpenUrlAbstract/FREE Full Text
  95. ↵
    1. Reid R,
    2. Hayes J
    (2003) Mechanisms and control of nutrient uptake in plants. Int Rev Cytol 229: 73–114
    OpenUrlPubMed
  96. ↵
    1. Rellán-Álvarez R,
    2. Lobet G,
    3. Lindner H,
    4. Pradier P-L,
    5. Sebastian J,
    6. Yee M-C,
    7. Geng Y,
    8. Trontin C,
    9. LaRue T,
    10. Schrager-Lavelle A, et al.
    (2015) GLO-Roots: An imaging platform enabling multidimensional characterization of soil-grown root systems. eLife 4: e07597
    OpenUrlCrossRef
  97. ↵
    1. Sakurai G,
    2. Satake A,
    3. Yamaji N,
    4. Mitani-Ueno N,
    5. Yokozawa M,
    6. Feugier FG,
    7. Ma JF
    (2015) In silico simulation modeling reveals the importance of the Casparian strip for efficient silicon uptake in rice roots. Plant Cell Physiol 56: 631–639
    OpenUrlCrossRefPubMed
  98. ↵
    1. Salt DE
    (2004) Update on plant ionomics. Plant Physiol 136: 2451–2456
    OpenUrlFREE Full Text
  99. ↵
    1. Segura V,
    2. Vilhjálmsson BJ,
    3. Platt A,
    4. Korte A,
    5. Seren Ü,
    6. Long Q,
    7. Nordborg M
    (2012) An efficient multi-locus mixed-model approach for genome-wide association studies in structured populations. Nat Genet 44: 825–830
    OpenUrlCrossRefPubMed
  100. ↵
    1. Shabala S,
    2. Shabala L,
    3. Bose J,
    4. Cuin T,
    5. Newman I
    (2013) Ion flux measurements using the MIFE technique. Methods Mol Biol 953: 171–183
    OpenUrlPubMed
  101. ↵
    1. Shaw A,
    2. Takacs I,
    3. Pagilla K,
    4. Riffat R,
    5. DeClippeleir H,
    6. Wilson C,
    7. Murthy S
    (2015) Toward universal half-saturation coefficients: Describing extant KS as a function of diffusion. Water Environ Res 87: 387–391
    OpenUrl
  102. ↵
    1. Shaw R,
    2. Williams AP,
    3. Jones DL
    (2014) Assessing soil nitrogen availability using microdialysis-derived diffusive flux measurements. Soil Sci Soc Am J 78: 1797–1803
    OpenUrlCrossRef
  103. ↵
    1. Siddiqi MY,
    2. Glass ADM,
    3. Ruth TJ
    (1990) Studies of the uptake of nitrate in barley: III. Compartmentation of NO3−. J Exp Bot 42: 1455–1463
    OpenUrl
  104. ↵
    1. Siddiqi MY,
    2. Glass AD,
    3. Ruth TJ,
    4. Fernando M
    (1989) Studies of the regulation of nitrate influx by barley seedlings using 13NO3−. Plant Physiol 90: 806–813
    OpenUrlAbstract/FREE Full Text
  105. ↵
    1. Silberbush M,
    2. Barber SA
    (1983) Sensitivity of simulated phosphorus uptake to parameters used by a mechanistic-mathematical model. Plant Soil 74: 93–100
    OpenUrlCrossRef
  106. ↵
    1. Sohlenkamp C,
    2. Shelden M,
    3. Howitt S,
    4. Udvardi M
    (2000) Characterization of Arabidopsis AtAMT2, a novel ammonium transporter in plants. FEBS Lett 467: 273–278
    OpenUrlCrossRefPubMed
  107. ↵
    1. Sorgonà A,
    2. Lupini A,
    3. Mercati F,
    4. Di Dio L,
    5. Sunseri F,
    6. Abenavoli MR
    (2011) Nitrate uptake along the maize primary root: An integrated physiological and molecular approach. Plant Cell Environ 34: 1127–1140
    OpenUrlCrossRefPubMed
  108. ↵
    1. Sun J,
    2. Bankston JR,
    3. Payandeh J,
    4. Hinds TR,
    5. Zagotta WN,
    6. Zheng N
    (2014) Crystal structure of the plant dual-affinity nitrate transporter NRT1.1. Nature 507: 73–77
    OpenUrlCrossRefPubMed
  109. ↵
    1. Sze H,
    2. Li X,
    3. Palmgren MG
    (1999) Energization of plant cell membranes by H+-pumping ATPases. Regulation and biosynthesis. Plant Cell 11: 677–690
    OpenUrlFREE Full Text
  110. ↵
    1. Takahashi H,
    2. Buchner P,
    3. Yoshimoto N,
    4. Hawkesford MJ,
    5. Shiu S-H
    (2012) Evolutionary relationships and functional diversity of plant sulfate transporters. Front Plant Sci 2: 119
    OpenUrlCrossRefPubMed
  111. ↵
    1. Teo YH,
    2. Beyrouty CA,
    3. Gbur EE
    (1992) Nitrogen, phosphorus, and potassium influx kinetic parameters of three rice cultivars. J Plant Nutr 15: 435–444
    OpenUrl
  112. ↵
    1. Thellier M
    (1970) An electrokinetic interpretation of the functioning of biological systems and its application to the study of mineral salts absorption. Ann Bot (Lond) 34: 983–1009
    OpenUrlCrossRef
  113. ↵
    1. Tisdall JM,
    2. Oades JM
    (1982) Organic matter and water-stable aggregates in soils. Eur J Soil Sci 33: 141–163
    OpenUrl
  114. ↵
    1. Trevisan S,
    2. Borsa P,
    3. Botton A,
    4. Varotto S,
    5. Malagoli M,
    6. Ruperti B,
    7. Quaggiotti S
    (2008) Expression of two maize putative nitrate transporters in response to nitrate and sugar availability. Plant Biol (Stuttg) 10: 462–475
    OpenUrlCrossRefPubMed
  115. ↵
    1. Tsay Y-F,
    2. Chiu C-C,
    3. Tsai C-B,
    4. Ho C-H,
    5. Hsu P-K
    (2007) Nitrate transporters and peptide transporters. FEBS Lett 581: 2290–2300
    OpenUrlCrossRefPubMed
  116. ↵
    1. DW Rains
    1. Veen BW
    (1980) Energy cost of ion transport. In DW Rains, ed, Genetic Engineering of Osmoregulation. Springer, Berlin, pp 187–198
  117. ↵
    1. Waines JG,
    2. Ehdaie B
    (2007) Domestication and crop physiology: roots of green-revolution wheat. Ann Bot 100: 991–998
    OpenUrlCrossRefPubMed
  118. ↵
    1. Wang Y-Y,
    2. Hsu P-K,
    3. Tsay Y-F
    (2012) Uptake, allocation and signaling of nitrate. Trends Plant Sci 17: 458–467
    OpenUrlCrossRefPubMed
  119. ↵
    1. Wendling M,
    2. Büchi L,
    3. Amossé C,
    4. Jeangros B,
    5. Walter A,
    6. Charles R
    (2017) Specific interactions leading to transgressive overyielding in cover crop mixtures. Agric Ecosyst Environ 17: 458–467
    OpenUrl
  120. ↵
    1. Yang M,
    2. Lu K,
    3. Zhao FJ,
    4. Xie W,
    5. Ramakrishna P,
    6. Wang G,
    7. Du Q,
    8. Liang L,
    9. Sun C,
    10. Zhao H, et al.
    (2018) Genome-wide association studies reveal the genetic basis of ionomic variation in rice. Plant Cell 30: 2720–2740
    OpenUrlAbstract/FREE Full Text
  121. ↵
    1. York LM
    (2019) Functional phenomics: An emerging field integrating high-throughput phenotyping, physiology, and bioinformatics. J Exp Bot 70: 379–386
    OpenUrl
  122. ↵
    1. York LM,
    2. Carminati A,
    3. Mooney SJ,
    4. Ritz K,
    5. Bennett MJ
    (2016) The holistic rhizosphere: integrating zones, processes, and semantics in the soil influenced by roots. J Exp Bot 67: 3629–3643
    OpenUrlCrossRefPubMed
  123. ↵
    1. York LM,
    2. Lynch JP
    (2015) Intensive field phenotyping of maize (Zea mays L.) root crowns identifies phenes and phene integration associated with plant growth and nitrogen acquisition. J Exp Bot 66: 5493–5505
    OpenUrlCrossRefPubMed
  124. ↵
    1. York LM,
    2. Nord EA,
    3. Lynch JP
    (2013) Integration of root phenes for soil resource acquisition. Front Plant Sci 4: 355
    OpenUrlPubMed
  125. ↵
    1. York LM,
    2. Silberbush M,
    3. Lynch JP
    (2016) Spatiotemporal variation of nitrate uptake kinetics within the maize (Zea mays L.) root system is associated with greater nitrate uptake and interactions with architectural phenes. J Exp Bot 67: 3763–3775
    OpenUrlCrossRefPubMed
  126. ↵
    1. Zelazny E,
    2. Vert G
    (2014) Plant nutrition: Root transporters on the move. Plant Physiol 166: 500–508
    OpenUrlAbstract/FREE Full Text
  127. ↵
    1. Zhan A,
    2. Lynch JP
    (2015) Reduced frequency of lateral root branching improves N capture from low-N soils in maize. J Exp Bot 66: 2055–2065
    OpenUrlCrossRefPubMed
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Targeting Root Ion Uptake Kinetics to Increase Plant Productivity and Nutrient Use Efficiency
Marcus Griffiths, Larry M. York
Plant Physiology Apr 2020, 182 (4) 1854-1868; DOI: 10.1104/pp.19.01496

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Targeting Root Ion Uptake Kinetics to Increase Plant Productivity and Nutrient Use Efficiency
Marcus Griffiths, Larry M. York
Plant Physiology Apr 2020, 182 (4) 1854-1868; DOI: 10.1104/pp.19.01496
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  • Article
    • Abstract
    • THEORETICAL FRAMEWORK
    • EXPERIMENTAL APPROACHES FOR STUDYING ROOT ION UPTAKE KINETICS
    • FUTURE PROSPECTS
    • Footnotes
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Plant Physiology: 182 (4)
Plant Physiology
Vol. 182, Issue 4
Apr 2020
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