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Pyruvate Dehydrogenase Complex from Chloroplasts of Pisum sativum L

Michael Williams, Douglas D. Randall
Michael Williams
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Douglas D. Randall
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Published December 1979. DOI: https://doi.org/10.1104/pp.64.6.1099

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Abstract

Pyruvate dehydrogenase complex is associated with intact chloroplasts and mitochondria of 9-day-old Pisum sativum L. seedlings. The ratio of the mitochondrial complex to the chloroplast complex activities is about 3 to 1. Maximal rates observed for chloroplast pyruvate dehydrogenase complex activity ranged from 6 to 9 micromoles of NADH produced per milligram of chlorophyll per hour. Osmotic rupture of pea chloroplasts released 88% of the complex activity, indicating that chloroplast pyruvate dehydrogenase complex is a stromal complex. The pH optimum for chloroplast pyruvate dehydrogenase complex was between 7.8 and 8.2, whereas the mitochondrial pyruvate dehydrogenase complex had a pH optimum between 7.3 and 7.7. Chloroplast pyruvate dehydrogenase complex activity was specific for pyruvate, dependent upon coenzyme A and NAD and partially dependent upon Mg2+ and thiamine pyrophosphate.

Chloroplast-associated pyruvate dehydrogenase complex provides a direct link between pyruvate metabolism and chloroplast fatty acid biosynthesis by providing the substrate, acetyl-CoA, necessary for membrane development in young plants.

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Pyruvate Dehydrogenase Complex from Chloroplasts of Pisum sativum L
Michael Williams, Douglas D. Randall
Plant Physiology Dec 1979, 64 (6) 1099-1103; DOI: 10.1104/pp.64.6.1099

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Pyruvate Dehydrogenase Complex from Chloroplasts of Pisum sativum L
Michael Williams, Douglas D. Randall
Plant Physiology Dec 1979, 64 (6) 1099-1103; DOI: 10.1104/pp.64.6.1099
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Plant Physiology
Vol. 64, Issue 6
December 1979
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