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A Mutant of Arabidopsis Deficient in C18:3 and C16:3 Leaf Lipids

John Browse, Peter McCourt, Chris Somerville
John Browse
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Peter McCourt
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Chris Somerville
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Published July 1986. DOI: https://doi.org/10.1104/pp.81.3.859

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  • © 1986 American Society of Plant Biologists

Abstract

Leaf tissue of a mutant of Arabidopsis thaliana contains reduced levels of both 16:3 and 18:3 fatty acids and has correspondingly increased levels of the 16:2 and 18:2 precursors due to a single recessive nuclear mutation. The kinetics of in vivo labeling of lipids with [14C]acetate and quantitative analysis of the fatty acid compositions of individual lipids suggests that reduced activity of a glycerolipid n-3 desaturase is responsible for the altered lipid composition of the mutant. The effects of the mutation are most pronounced when plants are grown at temperatures above 26°C but are relatively minor below 18°C, suggesting a temperature-sensitive enzyme. Since the desaturation of both 16- and 18-carbon fatty acids is altered, it appears that the affected enzyme lacks specificity with respect to acyl group chain length and that it is located in the chloroplast where 16:3-monogalactosyldiglyceride is synthesized. Because the degree of unsaturation of all the major glycerolipids was similarly affected by the mutation, it is inferred that either the affected desaturase does not exhibit head group specificity or there is substantial transfer of trienoic acyl groups between different lipid classes. Both chloroplast and extrachloroplast lipids are equally affected by the mutation. Thus, either the desaturase is located both outside and inside the chloroplast, or 18:3 formed inside the chloroplast is reexported to other cellular sites.

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A Mutant of Arabidopsis Deficient in C18:3 and C16:3 Leaf Lipids
John Browse, Peter McCourt, Chris Somerville
Plant Physiology Jul 1986, 81 (3) 859-864; DOI: 10.1104/pp.81.3.859

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A Mutant of Arabidopsis Deficient in C18:3 and C16:3 Leaf Lipids
John Browse, Peter McCourt, Chris Somerville
Plant Physiology Jul 1986, 81 (3) 859-864; DOI: 10.1104/pp.81.3.859
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Plant Physiology
Vol. 81, Issue 3
July 1986
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