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Research ArticleEnvironmental and Stress Physiology
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Plasma Membrane Lipid Alterations Associated with Cold Acclimation of Winter Rye Seedlings (Secale cereale L. cv Puma)

Daniel V. Lynch, Peter L. Steponkus
Daniel V. Lynch
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Peter L. Steponkus
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Published April 1987. DOI: https://doi.org/10.1104/pp.83.4.761

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Abstract

Highly enriched plasma membrane fractions were isolated from leaves of nonacclimated (NA) and acclimated (ACC) rye (Secale cereale L. cv Puma) seedlings. Collectively, free sterols, steryl glucosides, and acylated steryl glucosides constituted >50 mole% of the total lipid in both NA and ACC plasma membrane fractions. Glucocerebrosides containing hydroxy fatty acids constituted the major glycolipid class of the plasma membrane, accounting for 16 mole% of the total lipid. Phospholipids, primarily phosphatidylcholine and phosphatidylethanolamine with lesser amounts of phosphatidylglycerol, phosphatidic acid, phosphatidylserine, and phosphatidylinositol, comprised only 32 mole% of the total lipid in NA samples. Following cold acclimation, free sterols increased from 33 to 44 mole%, while steryl glucosides and acylated steryl glucosides decreased from 15 to 6 mole% and 4 to 1 mole%, respectively. Sterol analyses of these lipid classes demonstrated that free β-sitosterol increased from 21 to 32 mole% (accounting for the increase in free sterols as a class) at the expense of sterol derivatives containing β-sitosterol. Glucocerebrosides decreased from 16 to 7 mole% of the total lipid following cold acclimation. In addition, the relative proportions of associated hydroxy fatty acids, including 22:0 (h), 24:0 (h), 22:1 (h), and 24:1 (h), were altered. The phospholipid content of the plasma membrane fraction increased to 42 mole% of the total lipid following cold acclimation. Although the relative proportions of the individual phospholipids did not change appreciably after cold acclimation, there were substantial differences in the molecular species. Di-unsaturated molecular species (18:2/18:2, 18:2/18:3, 18:3/18:3) of phosphatidylcholine and phosphatidylethanolamine increased following acclimation. These results demonstrate that cold acclimation results in substantial changes in the lipid composition of the plasma membrane.

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Plasma Membrane Lipid Alterations Associated with Cold Acclimation of Winter Rye Seedlings (Secale cereale L. cv Puma)
Daniel V. Lynch, Peter L. Steponkus
Plant Physiology Apr 1987, 83 (4) 761-767; DOI: 10.1104/pp.83.4.761

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Plasma Membrane Lipid Alterations Associated with Cold Acclimation of Winter Rye Seedlings (Secale cereale L. cv Puma)
Daniel V. Lynch, Peter L. Steponkus
Plant Physiology Apr 1987, 83 (4) 761-767; DOI: 10.1104/pp.83.4.761
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Plant Physiology
Vol. 83, Issue 4
April 1987
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  • Enhancement of Na+ Uptake Currents, Time-Dependent Inward-Rectifying K+ Channel Currents, and K+Channel Transcripts by K+ Starvation in Wheat Root Cells
  • The Effects of Elevated CO2 Concentrations on Cell Division Rates, Growth Patterns, and Blade Anatomy in Young Wheat Plants Are Modulated by Factors Related to Leaf Position, Vernalization, and Genotype
  • Iron-Superoxide Dismutase Expression in Transgenic Alfalfa Increases Winter Survival without a Detectable Increase in Photosynthetic Oxidative Stress Tolerance
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