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Research ArticleMembranes and Bioenergetics
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Calcium Transport in Sealed Vesicles from Red Beet (Beta vulgaris L.) Storage Tissue

II. Characterization of 45Ca2+ Uptake into Plasma Membrane Vesicles

John L. Giannini, Jose Ruiz-Cristin, Donald P. Briskin
John L. Giannini
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Jose Ruiz-Cristin
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Donald P. Briskin
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Published December 1987. DOI: https://doi.org/10.1104/pp.85.4.1137

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Abstract

Calcium uptake was examined in sealed plasma membrane vesicles isolated from red beet (Beta vulgaris L.) storage tissue using 45Ca2+. Uptake of 45Ca2+ by the vesicles was ATP-dependent and radiotracer accumulated by the vesicles could be released by the addition of the calcium ionophore A23187. The uptake was stimulated by gramicidin D but slightly inhibited by carbonylcyanide m-chlorophenylhydrazone. Although the latter result might suggest some degree of indirect coupling of 45Ca2+ uptake to ATP utilization via δμH+, no evidence for a secondary H+/Ca2+ antiport in this vesicle system could be found. Following the imposition of an acid-interior pH gradient, proton efflux from the vesicle was not enhanced by the addition of Ca2+ and an imposed pH gradient could not drive 45Ca2+ uptake. Optimal uptake of 45Ca2+ occurred broadly between pH 7.0 and 7.5 and the transport was inhibited by orthovanadate, N,N′-dicyclohexylcarbodiimide, and diethylstilbestrol but insensitive to nitrate and azide. The dependence of 45Ca2+ uptake on both calcium and Mg:ATP concentration demonstrated saturation kinetics with Km values of 6 micromolar and 0.37 millimolar, respectively. While ATP was the preferred substrate for driving 45Ca2+ uptake, GTP could drive transport at about 50% of the level observed for ATP. The results of this study demonstrate the presence of a unique primary calcium transport system associated with the plasma membrane which could drive calcium efflux from the plant cell.

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Calcium Transport in Sealed Vesicles from Red Beet (Beta vulgaris L.) Storage Tissue
John L. Giannini, Jose Ruiz-Cristin, Donald P. Briskin
Plant Physiology Dec 1987, 85 (4) 1137-1142; DOI: 10.1104/pp.85.4.1137

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Calcium Transport in Sealed Vesicles from Red Beet (Beta vulgaris L.) Storage Tissue
John L. Giannini, Jose Ruiz-Cristin, Donald P. Briskin
Plant Physiology Dec 1987, 85 (4) 1137-1142; DOI: 10.1104/pp.85.4.1137
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Plant Physiology
Vol. 85, Issue 4
December 1987
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More in this TOC Section

  • Effects of Deuterium Oxide on Growth, Proton Extrusion, Potassium Influx, and in Vitro Plasma Membrane Activities in Maize Root Segments
  • Electrically Induced Protoplast Fusion Using Pulse Electric Fields for Dielectrophoresis
  • Isolation and Characterization of the Tricarboxylate Transporter from Pea Mitochondria
Show more Membranes and Bioenergetics

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