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Research ArticleMicrobe-Plant Interactions
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Pathogenesis-Related Proteins of Tomato

P-69 as an Alkaline Endoproteinase

Pablo Vera, Vicente Conejero
Pablo Vera
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Vicente Conejero
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Published May 1988. DOI: https://doi.org/10.1104/pp.87.1.58

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  • © 1988 American Society of Plant Biologists

Abstract

An endoproteinase induced by citrus exocortis viroid has been purified from tomato (Lycopersicon esculentum Mill, cv “Rutgers”) leaves. The proteinase corresponds to one of the major pathogenesis-related proteins of tomato plants and was designated proteinase P-69 as it has a molecular weight of 69,000 to 70,000. The proteinase was purified in four steps: (NH4)2SO4 fractionation, chromatography on Bio-Gel P-60, DEAE-Sepharose chromatography, and casein-Sepharose affinity chromatography. The proteinase had a pH optimum of 8.5 to 9.0 when assayed with either fluorescein thiocarbamoyl derivative (FTC)-casein or FTC-ribulose 1,5-bisphosphate carboxylase/oxygenase as substrates. The proteinase activity was inhibited by pCMB and strongly activated by calcium and magnesium ions as well as by DTT. When analyzed by electrofocusing, the activity showed a pI around 9.0.

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Pathogenesis-Related Proteins of Tomato
Pablo Vera, Vicente Conejero
Plant Physiology May 1988, 87 (1) 58-63; DOI: 10.1104/pp.87.1.58

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Pathogenesis-Related Proteins of Tomato
Pablo Vera, Vicente Conejero
Plant Physiology May 1988, 87 (1) 58-63; DOI: 10.1104/pp.87.1.58
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Plant Physiology
Vol. 87, Issue 1
May 1988
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More in this TOC Section

  • Observation of the Oxygen Diffusion Barrier in Soybean (Glycine max) Nodules with Magnetic Resonance Microscopy
  • Exogenous Ethylene Inhibits Nodulation of Pisum sativum L. cv Sparkle
  • Reversible O2 Inhibition of Nitrogenase Activity in Attached Soybean Nodules
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