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Research ArticleMolecular Biology and Gene Regulation
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Reduction of Turgor Induces Rapid Changes in Leaf Translatable RNA

Felix D. Guerrero, John E. Mullet
Felix D. Guerrero
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John E. Mullet
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Published October 1988. DOI: https://doi.org/10.1104/pp.88.2.401

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Abstract

The turgor of pea (Pisum sativum) leaves was reduced by exposing excised pea shoots to a stream of 23°C air for 20 min. Poly(A)+ RNA was isolated from control and wilted shoots, translated in vitro and radiolabeled translation products separated by electrophoresis on two-dimensional (isoelectric focusing-sodium dodecyl sulfate) polyacrylamide gels. This analysis showed that the levels of several poly(A)+ RNAs increased in wilted plants. Most of the poly(A)+ RNAs induced in wilted plants did not accumulate in response to heat shock or exogenously applied ABA even though endogenous ABA levels were found to increase in shoots 30 min after wilting and by 4 h had increased 50-fold (1 versus 0.02 microgram per gram fresh weight). A λgt10 cDNA library was constructed using poly(A)+ RNA from wilted shoots which had been incubated for 4 hours. Differential screening of the library identified four clones corresponding to poly(A)+ RNAs which are induced in wilted shoots.

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Reduction of Turgor Induces Rapid Changes in Leaf Translatable RNA
Felix D. Guerrero, John E. Mullet
Plant Physiology Oct 1988, 88 (2) 401-408; DOI: 10.1104/pp.88.2.401

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Reduction of Turgor Induces Rapid Changes in Leaf Translatable RNA
Felix D. Guerrero, John E. Mullet
Plant Physiology Oct 1988, 88 (2) 401-408; DOI: 10.1104/pp.88.2.401
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Plant Physiology
Vol. 88, Issue 2
October 1988
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More in this TOC Section

  • Genes Galore: A Summary of Methods for Accessing Results from Large-Scale Partial Sequencing of Anonymous Arabidopsis cDNA Clones
  • Expression of the Arabidopsis Gene Akr Coincides with Chloroplast Development
  • Molecular Genetics of the Maize (Zea mays L.) Aspartate Kinase-Homoserine Dehydrogenase Gene Family
Show more Molecular Biology and Gene Regulation

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