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Research ArticleMolecular Biology and Gene Regulation
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Bean Abscission Cellulase

Characterization of a cDNA Clone and Regulation of Gene Expression by Ethylene and Auxin

Mark L. Tucker, Roy Sexton, Elena del Campillo, Lowell N. Lewis
Mark L. Tucker
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Roy Sexton
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Elena del Campillo
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Lowell N. Lewis
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Published December 1988. DOI: https://doi.org/10.1104/pp.88.4.1257

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Abstract

The physiology and anatomy of abscission has been studied in considerable detail; however, information on the regulation of gene expression in abscission has been limited because of a lack of probes for specific genes. We have identified and sequenced a 595 nucleotide bean (Phaseolus vulgaris cv Red Kidney) abscission cellulase cDNA clone (pBACl). The bean cellulase cDNA has extensive nucleic and amino acid sequence identity with the avocado cellulase cDNA pAV363. The 2.0 kilobase bean mRNA complementary to pBACl codes for a polypeptide of approximately 51 kilodalton (shown by hybrid-selection followed by in vitro translation). Bean cellulase antiserum is shown to immunoprecipitate a 51 kilodalton polypeptide from the in vitro translation products of abscission zone poly(A)+ RNA. Ethylene initiates bean leaf abscission and tissue-specific expression of cellulase mRNA. If ethylene treatment of bean explants was discontinued after 31 h and then 2,5-norbornadiene given to inhibit responses resulting from endogenously synthesized ethylene, polysomal cellulase mRNA hybridizing to pBACl decreased. Thus, ethylene is required not only to initiate abscission and cellulase gene expression but also to maintain continued accumulation of cellulase mRNA. Explants treated with auxin 4 hours prior to a 48 hour treatment with ethylene showed no substantial accumulation of RNA hybridizing to pBACl or expression of cellulase activity.

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Bean Abscission Cellulase
Mark L. Tucker, Roy Sexton, Elena del Campillo, Lowell N. Lewis
Plant Physiology Dec 1988, 88 (4) 1257-1262; DOI: 10.1104/pp.88.4.1257

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Bean Abscission Cellulase
Mark L. Tucker, Roy Sexton, Elena del Campillo, Lowell N. Lewis
Plant Physiology Dec 1988, 88 (4) 1257-1262; DOI: 10.1104/pp.88.4.1257
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Plant Physiology
Vol. 88, Issue 4
December 1988
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More in this TOC Section

  • Genes Galore: A Summary of Methods for Accessing Results from Large-Scale Partial Sequencing of Anonymous Arabidopsis cDNA Clones
  • Expression of the Arabidopsis Gene Akr Coincides with Chloroplast Development
  • Molecular Genetics of the Maize (Zea mays L.) Aspartate Kinase-Homoserine Dehydrogenase Gene Family
Show more Molecular Biology and Gene Regulation

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