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Research ArticleCellular and Structural Biology
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Rapid Image Analysis Screening Procedure for Identifying Chloroplast Number Mutants in Mesophyll Cells of Arabidopsis thaliana (L.) Heynh.

Kevin A. Pyke, Rachel M. Leech
Kevin A. Pyke
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Rachel M. Leech
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Published August 1991. DOI: https://doi.org/10.1104/pp.96.4.1193

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Abstract

To analyze the genetic control of the process of chloroplast division, a direct image analysis screening procedure has been developed in which mutants of Arabidopsis thaliana (L.) Heynh. var Landsberg erecta are selected on the basis of abnormal chloroplast number. The selection procedure is based on image analysis thresholding after iodine staining, which facilitates the automatic counting of chloroplasts in isolated mesophyll cells. M2 seedlings are screened for significant deviation from the wild type relationship between mesophyll cell size and chloroplast number. Mutants with both abnormally high and abnormally low chloroplast numbers were identified. Of 3500 individual M2 seedlings screened, 18 mutant lines have been isolated and shown to be stably inherited in three subsequent generations. The most extreme phenotypes show an 80% reduction or a 50% increase in chloroplast number per mesophyll cell.

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Rapid Image Analysis Screening Procedure for Identifying Chloroplast Number Mutants in Mesophyll Cells of Arabidopsis thaliana (L.) Heynh.
Kevin A. Pyke, Rachel M. Leech
Plant Physiology Aug 1991, 96 (4) 1193-1195; DOI: 10.1104/pp.96.4.1193

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Rapid Image Analysis Screening Procedure for Identifying Chloroplast Number Mutants in Mesophyll Cells of Arabidopsis thaliana (L.) Heynh.
Kevin A. Pyke, Rachel M. Leech
Plant Physiology Aug 1991, 96 (4) 1193-1195; DOI: 10.1104/pp.96.4.1193
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Plant Physiology
Vol. 96, Issue 4
August 1991
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  • The Isolation of Actin from Pea Roots by DNase I Affinity Chromatography
  • Fourier Transform Infrared Microspectroscopy Is a New Way to Look at Plant Cell Walls
  • Flow Cytometry of Spinach Chloroplasts
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