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Research ArticleMetabolism and Enzymology
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Amino Acid and Sucrose Content Determined in the Cytosolic, Chloroplastic, and Vacuolar Compartments and in the Phloem Sap of Spinach Leaves

Burgi Riens, Gertrud Lohaus, Dieter Heineke, Hans Walter Heldt
Burgi Riens
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Gertrud Lohaus
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Dieter Heineke
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Hans Walter Heldt
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Published September 1991. DOI: https://doi.org/10.1104/pp.97.1.227

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Abstract

Amino acid and sucrose contents were analyzed in the chloroplastic, cytosolic, and vacuolar compartments and in the phloem sap of illuminated spinach leaves (Spinacia oleracea L.). The determination of subcellular metabolite distribution was carried out by nonaqueous fractionation of frozen and lyophilized leaf material using a novel three-compartment calculation method. The phloem sap was collected by aphid stylets which had been severed by a laser beam. Subcellular analysis revealed that the amino acids found in leaves are located mainly in the chloroplast stroma and in the cytosol, the sum of their concentrations amounting to 151 and 121 millimolar, respectively, whereas the amino acid concentrations in the vacuole are one order of magnitude lower. The amino acid concentrations in the phloem sap are found to be not very different from the cytosolic concentrations, whereas the sieve tube concentration of sucrose is found to be one order of magnitude higher than in the cytosol. It is concluded that the phloem loading results in a preferential extraction of sucrose from the source cells.

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Amino Acid and Sucrose Content Determined in the Cytosolic, Chloroplastic, and Vacuolar Compartments and in the Phloem Sap of Spinach Leaves
Burgi Riens, Gertrud Lohaus, Dieter Heineke, Hans Walter Heldt
Plant Physiology Sep 1991, 97 (1) 227-233; DOI: 10.1104/pp.97.1.227

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Amino Acid and Sucrose Content Determined in the Cytosolic, Chloroplastic, and Vacuolar Compartments and in the Phloem Sap of Spinach Leaves
Burgi Riens, Gertrud Lohaus, Dieter Heineke, Hans Walter Heldt
Plant Physiology Sep 1991, 97 (1) 227-233; DOI: 10.1104/pp.97.1.227
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Plant Physiology
Vol. 97, Issue 1
September 1991
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More in this TOC Section

  • Identification of a Gene that Complements an Arabidopsis Mutant Deficient in Chloroplast [omega]6 Desaturase Activity
  • Tomato Fruit Polygalacturonase Isozyme 1 (Characterization of the [beta] Subunit and Its State of Assembly in Vivo)
  • A Novel Metabolic Pathway for Indole-3-Acetic Acid in Apical Shoots of Populus tremula (L.) x Populus tremuloides (Michx.)
Show more Metabolism and Enzymology

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