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Research ArticleMembranes and Bioenergetics
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Tissue-Specific Expression of the Alternative Oxidase in Soybean and Siratro

Anthony Kearns, James Whelan, Susan Young, Thomas E. Elthon, David A. Day
Anthony Kearns
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James Whelan
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Susan Young
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Thomas E. Elthon
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David A. Day
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Published June 1992. DOI: https://doi.org/10.1104/pp.99.2.712

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  • © 1992 American Society of Plant Biologists

Abstract

Alternative oxidase activity (cyanide-insensitive respiration) was measured in mitochondria from the shoots, roots, and nodules of soybean (Glycine max L.) and siratro (Macroptilium atropurpureum) plants. Activity was highest in the shoots and lowest in the nodules. Alternative oxidase activity was associated with one (roots) or two (shoots) proteins between 30 and 35 kilodaltons that were detected by western blotting with a monoclonal antibody against Sauromatum guttatum alternative oxidase. No such protein was detected in nodule mitochondria. Measurements of oxygen uptake by isolated soybean root and nodule cells in the presence of cyanide and salicylhydroxamic acid indicated that alternative oxidase activity was confined to the uninfected cortex cells of the nodule. Immunoprecipitation of translation products of mRNA isolated from soybean shoots revealed a major band at 43 kilodaltons that is assumed to be the precursor of an alternative oxidase protein. This band was not seen when mRNA from nodules was treated in the same fashion. The results indicate that tissue-specific expression of the alternative oxidase occurs in soybean and siratro.

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Tissue-Specific Expression of the Alternative Oxidase in Soybean and Siratro
Anthony Kearns, James Whelan, Susan Young, Thomas E. Elthon, David A. Day
Plant Physiology Jun 1992, 99 (2) 712-717; DOI: 10.1104/pp.99.2.712

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Tissue-Specific Expression of the Alternative Oxidase in Soybean and Siratro
Anthony Kearns, James Whelan, Susan Young, Thomas E. Elthon, David A. Day
Plant Physiology Jun 1992, 99 (2) 712-717; DOI: 10.1104/pp.99.2.712
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Plant Physiology
Vol. 99, Issue 2
June 1992
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More in this TOC Section

  • Effects of Deuterium Oxide on Growth, Proton Extrusion, Potassium Influx, and in Vitro Plasma Membrane Activities in Maize Root Segments
  • Electrically Induced Protoplast Fusion Using Pulse Electric Fields for Dielectrophoresis
  • Isolation and Characterization of the Tricarboxylate Transporter from Pea Mitochondria
Show more Membranes and Bioenergetics

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