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Research ArticleMembranes and Bioenergetics
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Transport Interactions between Paraquat and Polyamines in Roots of Intact Maize Seedlings

Jonathan J. Hart, Joseph M. DiTomaso, Dean L. Linscott, Leon V. Kochian
Jonathan J. Hart
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Joseph M. DiTomaso
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Dean L. Linscott
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Leon V. Kochian
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Published August 1992. DOI: https://doi.org/10.1104/pp.99.4.1400

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Abstract

Interactions between absorption of paraquat and the polyamines putrescine, cadaverine, and spermine in roots of intact maize (Zea mays L. cv 3377 Pioneer) seedlings were examined. Concentration-dependent kinetics for paraquat and putrescine influx were similar and both kinetic curves could be resolved into a linear and a saturable component. The linear component was previously shown to represent cell wall/membrane binding. The saturable components for paraquat and putrescine uptake, which represent influx across the plasmalemma, had Km values of 98 and 120 micromolar, respectively, and Vmax values of 445 and 456 nanomoles per gram fresh weight per hour, respectively. Lineweaver-Burk transformation of the saturable component of paraquat influx in the presence of varying concentrations of putrescine indicated that the diamine competitively inhibited the saturable component of paraquat uptake. Reciprocal experiments similarly demonstrated that paraquat competitively inhibited the saturable component of putrescine uptake. Competitive inhibition of both paraquat and putrescine influx could also be demonstrated with the diamine cadaverine, which has a charge distribution similar to that of paraquat and putrescine. In contrast, the larger, tetravalent polyamine spermine appeared to noncompetitively inhibit the influx of paraquat and putrescine. These results strongly suggest that paraquat enters maize root cells via a carrier system that normally functions in the transport of diamines with a charge distribution similar to that of paraquat.

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Transport Interactions between Paraquat and Polyamines in Roots of Intact Maize Seedlings
Jonathan J. Hart, Joseph M. DiTomaso, Dean L. Linscott, Leon V. Kochian
Plant Physiology Aug 1992, 99 (4) 1400-1405; DOI: 10.1104/pp.99.4.1400

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Transport Interactions between Paraquat and Polyamines in Roots of Intact Maize Seedlings
Jonathan J. Hart, Joseph M. DiTomaso, Dean L. Linscott, Leon V. Kochian
Plant Physiology Aug 1992, 99 (4) 1400-1405; DOI: 10.1104/pp.99.4.1400
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Plant Physiology
Vol. 99, Issue 4
August 1992
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More in this TOC Section

  • Short-Term Experiments on Ion Transport by Seedlings and Excised Roots
  • Photosystem II Core Phosphorylation Heterogeneity, Differential Herbicide Binding, and Regulation of Electron Transfer in Photosystem II Preparations from Spinach
  • Effects of Deuterium Oxide on Growth, Proton Extrusion, Potassium Influx, and in Vitro Plasma Membrane Activities in Maize Root Segments
Show more Membranes and Bioenergetics

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