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Research ArticleMetabolism and Enzymology
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Subcellular Location of Δ1-Pyrroline-5-Carboxylate Reductase in Root/Nodule and Leaf of Soybean

Annamaria Szoke, Guo-Hua Miao, Zonglie Hong, Desh Pal S. Verma
Annamaria Szoke
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Guo-Hua Miao
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Zonglie Hong
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Desh Pal S. Verma
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Published August 1992. DOI: https://doi.org/10.1104/pp.99.4.1642

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Abstract

The expression of Δ1-pyrroline-5-carboxylate reductase (P5CR) gene was found to be higher in soybean root nodules than in leaves and roots, and its expression in roots appeared to be osmoregulated (AJ Delauney, DPS Verma [1990] Mol Gen Genet 221: 299-305). P5CR was purified to homogeneity as a monomeric protein of 29 kilodaltons by overexpression of a soybean P5CR cDNA clone in Escherichia coli. The pH optimum of the purified P5CR was altered by increasing the salt concentration, and maximum enzyme activity was attainable at a lower pH under high salt (0.2-1 molar NaCl). Kinetic studies of the purified enzyme suggested that nicotinamide adenine dinucleotide phosphate+ inhibited P5CR activity, whereas nicotinamide adenine dinucleotide+ did not. Subcellular fractionation and antibodies raised against purified soybean P5CR were used to investigate location of the enzyme in different parts of soybean as well as in leaves of transgenic tobacco plants synthesizing soybean P5CR. P5CR activity was present in cytoplasm of soybean roots and nodules as well as in leaves, but in leaves, about 15% of the activity was detected in the plastid fraction. The location of P5CR was further confirmed by western blot assay of the proteins from cytosol and plastid fractions of different parts of the plant. Expression of soybean nodule cytosolic P5CR in transgenic tobacco under the control of cauliflower mosaic virus 35S promoter led to the accumulation of this protein exclusively in the cytoplasm, suggesting that the chloroplastic activity may be due to the presence of a plastid form of the enzyme. The different locations of P5CR in root and leaf suggested that proline may be synthesized in different subcellular compartments in root and leaf. Proline concentration was not significantly increased in transgenic plants exhibiting high level P5CR activity, indicating that reduction of P5C is not a rate-limiting step in proline production.

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Subcellular Location of Δ1-Pyrroline-5-Carboxylate Reductase in Root/Nodule and Leaf of Soybean
Annamaria Szoke, Guo-Hua Miao, Zonglie Hong, Desh Pal S. Verma
Plant Physiology Aug 1992, 99 (4) 1642-1649; DOI: 10.1104/pp.99.4.1642

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Subcellular Location of Δ1-Pyrroline-5-Carboxylate Reductase in Root/Nodule and Leaf of Soybean
Annamaria Szoke, Guo-Hua Miao, Zonglie Hong, Desh Pal S. Verma
Plant Physiology Aug 1992, 99 (4) 1642-1649; DOI: 10.1104/pp.99.4.1642
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Plant Physiology
Vol. 99, Issue 4
August 1992
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More in this TOC Section

  • The Metabolism of Gibberellin A20 to Gibberellin A1 by Tall and Dwarf Mutants of Oryza sativa and Arabidopsis thaliana
  • A Mutation at the fad8 Locus of Arabidopsis Identifies a Second Chloroplast [omega]-3 Desaturase
  • The 58-Kilodalton Calmodulin-Binding Glutamate Decarboxylase Is a Ubiquitous Protein in Petunia Organs and Its Expression Is Developmentally Regulated
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