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Lignification in sugarcane: biochemical characterization, gene discovery and expression analysis in two genotypes contrasting for lignin content

Alexandra Bottcher, Igor Cesarino, Adriana B. Santos, Renato Vicentini, Juliana L.S. Mayer, Ruben Vanholme, Kris Morreel, Geert Goeminne, Jullyana Cristina M.S. Moura, Paula M. Nobile, Sandra Maria Carmello-Guerreiro, Ivan Antonio Anjos, Silvana Creste, Wout Boerjan, Marcos G.A. Landell, Paulo Mazzafera
Alexandra Bottcher
Universidade Estadual de Campinas;
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Igor Cesarino
Ghent University;
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Adriana B. Santos
Universidade Estadual de Campinas;
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Renato Vicentini
Universidade Estadual de Campinas;
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Juliana L.S. Mayer
Universidade Estadual do Centro-Oeste;
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Ruben Vanholme
Ghent University;
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Kris Morreel
Ghent University;
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Geert Goeminne
Ghent University;
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Jullyana Cristina M.S. Moura
Universidade Federal do Triangulo Mineiro;
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Paula M. Nobile
Universidade Estadual de Campinas;
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Sandra Maria Carmello-Guerreiro
Universidade Estadual de Campinas;
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Ivan Antonio Anjos
Instituto Agronomico de Campinas
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Silvana Creste
Instituto Agronomico de Campinas
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Wout Boerjan
Ghent University;
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Marcos G.A. Landell
Instituto Agronomico de Campinas
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Paulo Mazzafera
Universidade Estadual de Campinas;
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  • For correspondence: pmazza@unicamp.br

Published October 2013. DOI: https://doi.org/10.1104/pp.113.225250

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Abstract

Sugarcane is currently one of the most efficient crops in the production of first generation biofuels. However, the bagasse represents an additional abundant lignocellulosic resource that has the potential to increase the ethanol production per plant. To achieve a more efficient conversion of bagasse into ethanol, a better understanding of the main factors affecting biomass recalcitrance is needed. Because several studies have shown a negative effect of lignin on saccharification yield, the characterization of lignin biosynthesis, structure and deposition in sugarcane is an important goal. Here, we present the first systematic study of lignin deposition during sugarcane stem development, using histological, biochemical and transcriptional data derived from two sugarcane genotypes with contrasting lignin contents. Lignin amount and composition were determined in rind (outer) and pith (inner) tissues throughout stem development. In addition, the phenolic metabolome was analyzed by ultrahigh-performance liquid chromatography-mass spectrometry, which allowed the identification of 35 compounds related to the phenylpropanoid pathway and monolignol biosynthesis. Furthermore, the sugarcane EST Database was extensively surveyed to identify lignin biosynthetic gene homologs and the expression of all identified genes during the stem development was determined by qRT-PCR. Our data provide the first in-depth characterization of lignin biosynthesis in sugarcane and form the baseline for the rational metabolic engineering of sugarcane feedstock for bioenergy purposes.

  • Received July 19, 2013.
  • Accepted October 17, 2013.
  • Published October 21, 2013.
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Lignification in sugarcane: biochemical characterization, gene discovery and expression analysis in two genotypes contrasting for lignin content
Alexandra Bottcher, Igor Cesarino, Adriana B. Santos, Renato Vicentini, Juliana L.S. Mayer, Ruben Vanholme, Kris Morreel, Geert Goeminne, Jullyana Cristina M.S. Moura, Paula M. Nobile, Sandra Maria Carmello-Guerreiro, Ivan Antonio Anjos, Silvana Creste, Wout Boerjan, Marcos G.A. Landell, Paulo Mazzafera
Plant Physiology Oct 2013, pp.113.225250; DOI: 10.1104/pp.113.225250

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Lignification in sugarcane: biochemical characterization, gene discovery and expression analysis in two genotypes contrasting for lignin content
Alexandra Bottcher, Igor Cesarino, Adriana B. Santos, Renato Vicentini, Juliana L.S. Mayer, Ruben Vanholme, Kris Morreel, Geert Goeminne, Jullyana Cristina M.S. Moura, Paula M. Nobile, Sandra Maria Carmello-Guerreiro, Ivan Antonio Anjos, Silvana Creste, Wout Boerjan, Marcos G.A. Landell, Paulo Mazzafera
Plant Physiology Oct 2013, pp.113.225250; DOI: 10.1104/pp.113.225250
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Plant Physiology: 176 (4)
Plant Physiology
Vol. 176, Issue 4
Apr 2018
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