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OtherResearch Article
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A laser dissection-RNAseq analysis highlights the activation of cytokinin pathways by Nod factors in the Medicago truncatula root epidermis

Marie-Françoise Jardinaud, Stéphane Boivin, Nathalie Rodde, Olivier Catrice, Anna Kisiala, Agnes Lepage, Sandra Moreau, Brice Roux, Ludovic Cottret, Erika Sallet, Mathias Brault, R.J. Neil Emery, Jerome Gouzy, Florian Frugier, Pascal Gamas
Marie-Françoise Jardinaud
ENSAT-CNRS-INRA, LIPM CITY: castanet tolosan France [FR]
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Stéphane Boivin
CNRS / INRA / Universités Paris-Sud / Paris-Diderot / d'Evry, IPS2 CITY: Gif-sur-Yvette France [FR]
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Nathalie Rodde
CNRS-INRA, LIPM CITY: castanet tolosan France [FR]
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Olivier Catrice
CNRS-INRA, LIPM CITY: castanet tolosan France [FR]
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Anna Kisiala
Trent University CITY: Peterborough Canada [CA]
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Agnes Lepage
CNRS CITY: Castanet Tolosan France [FR]
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Sandra Moreau
CNRS-INRA, LIPM CITY: castanet tolosan France [FR]
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Brice Roux
CNRS-INRA, LIPM CITY: castanet tolosan France [FR]
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Ludovic Cottret
CNRS-INRA, LIPM CITY: Castanet Tolosan cedex France [FR]
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Erika Sallet
CNRS-INRA, LIPM CITY: Castanet Tolosan cedex France [FR]
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Mathias Brault
Institut des Sciences du V�g�tal UPR CNRS 2355, 1 avenue de la Terrasse CITY: Gif sur Yvette POSTAL_CODE: 91198 cedex France [FR]
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R.J. Neil Emery
Trent University CITY: Peterborough STATE: On POSTAL_CODE: K9J 7B8 Canada [CA]
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Jerome Gouzy
n/a n/a CITY: n/a POSTAL_CODE: n/a France [FR]
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Florian Frugier
CNRS CITY: Gif-sur-Yvette France [FR]
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Pascal Gamas
CNRS-INRA, LIPM CITY: Castanet Tolosan cedex POSTAL_CODE: 31326 France [FR]
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  • For correspondence: pascal.gamas@toulouse.inra.fr

Published May 2016. DOI: https://doi.org/10.1104/pp.16.00711

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Abstract

Nod factors (NF) are lipochitooligosaccharidic signal molecules produced by rhizobia, which play a key role in the rhizobium-legume symbiotic interaction. In this study, we analyzed the gene expression reprogramming induced by purified NF (4h and 24h of treatment) in the root epidermis of the model legume Medicago truncatula. Tissue-specific transcriptome analysis was achieved by laser capture micro-dissection coupled to high depth RNA sequencing. Expression of 17,191 genes was detected in the epidermis, among which 1070 were found to be regulated by NF addition, including previously characterized NF-induced marker genes. Many genes exhibited strong levels of transcriptional activation, sometimes only transiently at 4 hours, indicating highly dynamic regulation. Expression reprogramming affected a variety of cellular processes, including perception, signaling, regulation of gene expression, as well as cell wall, cytoskeleton, transport, metabolism and defense, with numerous NF-induced genes never identified before. Strikingly, early epidermal activation of cytokinin (CK) pathways was evidenced, based on the induction of CK metabolic and signaling genes, including the CRE1 receptor essential to promote nodulation. These transcriptional activations were independently validated using promoter:GUS fusions with the MtCRE1 CK receptor gene and a CK response reporter (TCSn). A CK pre-treatment reduced the NF-induction of the ENOD11 symbiotic marker, while a CK degrading enzyme (AtCKX3, for cytokinin oxidase/deshydrogenase 3) ectopically expressed in the root epidermis led to increased NF-induction of ENOD11 and nodulation. CK may therefore play both positive and negative roles in M. truncatula nodulation.

  • Received May 6, 2016.
  • Accepted May 18, 2016.
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Root epidermal cytokinins and Nod factor signaling
Marie-Françoise Jardinaud, Stéphane Boivin, Nathalie Rodde, Olivier Catrice, Anna Kisiala, Agnes Lepage, Sandra Moreau, Brice Roux, Ludovic Cottret, Erika Sallet, Mathias Brault, R.J. Neil Emery, Jerome Gouzy, Florian Frugier, Pascal Gamas
Plant Physiology May 2016, pp.00711.2016; DOI: 10.1104/pp.16.00711

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Root epidermal cytokinins and Nod factor signaling
Marie-Françoise Jardinaud, Stéphane Boivin, Nathalie Rodde, Olivier Catrice, Anna Kisiala, Agnes Lepage, Sandra Moreau, Brice Roux, Ludovic Cottret, Erika Sallet, Mathias Brault, R.J. Neil Emery, Jerome Gouzy, Florian Frugier, Pascal Gamas
Plant Physiology May 2016, pp.00711.2016; DOI: 10.1104/pp.16.00711
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Plant Physiology: 179 (2)
Plant Physiology
Vol. 179, Issue 2
Feb 2019
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