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Optical properties of corals distort variable chlorophyll fluorescence measurements

Daniel Wangpraseurt, Mads Lichtenberg, Steven L Jacques, Anthony William Larkum, Michael Kühl
Daniel Wangpraseurt
Universtiy of Cambridge CITY: Cambridge POSTAL_CODE: CB2 1EW United Kingdom [GB]
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  • For correspondence: dw527@cam.ac.uk
Mads Lichtenberg
Marine Biological Section, University of Copenhagen CITY: Helsingør Denmark [DK]
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Steven L Jacques
Oregon Health & Science University CITY: Portland STATE: Oregon United States Of America [US]
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Anthony William Larkum
University of Technology, Sydney CITY: Sydney STATE: NSW POSTAL_CODE: 2007 Australia [AU]
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Michael Kühl
Marine Biological Section, University of Copenhagen CITY: Helsingør Denmark [DK]
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Published January 2019. DOI: https://doi.org/10.1104/pp.18.01275

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  • {copyright, serif} 2019 American Society of Plant Biologists. All rights reserved.

Abstract

Pulse amplitude modulated (PAM) fluorimetry is widely used in photobiological studies of corals, as it rapidly provides numerous photosynthetic parameters to assess coral ecophysiology. Coral optics studies have revealed the presence of light gradients in corals, which are strongly affected by light scattering in coral tissue and skeleton. We investigated whether coral optics affects variable chlorophyll fluorescence measurements and derived photosynthetic parameters by developing planar hydrogel slabs with immobilized microalgae and with bulk optical properties similar to those of different types of corals. Our results show that PAM-based measurements of photosynthetic parameters differed substantially between hydrogels with different degrees of light scattering but identical microalgal density, yielding deviations in apparent maximal electron transport rates by a factor of 2. Furthermore, system settings such as the measuring light intensity affected F0, Fm and Fv/Fm in hydrogels with identical light absorption but different degrees of light scattering. Likewise, differences in microalgal density affected variable chlorophyll fluorescence parameters, where higher algal densities led to greater Fv/Fm values and relative electron transport rates. These results have important implications for the use of variable chlorophyll fluorimetry in ecophysiological studies of coral stress and photosynthesis, as well as other optically dense systems such as plant tissue and biofilms.

  • Received October 15, 2018.
  • Accepted January 16, 2019.

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Coral optics and variable chlorophyll fluorescence
Daniel Wangpraseurt, Mads Lichtenberg, Steven L Jacques, Anthony William Larkum, Michael Kühl
Plant Physiology Jan 2019, pp.01275.2018; DOI: 10.1104/pp.18.01275

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Coral optics and variable chlorophyll fluorescence
Daniel Wangpraseurt, Mads Lichtenberg, Steven L Jacques, Anthony William Larkum, Michael Kühl
Plant Physiology Jan 2019, pp.01275.2018; DOI: 10.1104/pp.18.01275
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Plant Physiology: 179 (2)
Plant Physiology
Vol. 179, Issue 2
Feb 2019
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