Skip to main content

Main menu

  • Authors
  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
    • Focus Collections
    • Classics Collection
    • Upcoming Focus Issues
  • Submit a Manuscript
  • Advertisers
  • About
    • About the Journal
    • Editorial Board and Staff
  • Subscribers
  • Librarians
  • More
    • Alerts
    • Contact Us
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Plant Cell Teaching Tools
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Physiology
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Plant Cell Teaching Tools
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Physiology

Advanced Search

  • Authors
  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
    • Focus Collections
    • Classics Collection
    • Upcoming Focus Issues
  • Submit a Manuscript
  • Advertisers
  • About
    • About the Journal
    • Editorial Board and Staff
  • Subscribers
  • Librarians
  • More
    • Alerts
    • Contact Us
  • Follow plantphysiol on Twitter
  • Visit plantphysiol on Facebook
  • Visit Plantae
Other
Open Access

Combinatorial Evolution of a Terpene Synthase Gene Cluster Explains Terpene Variations in Oryza

Hao Chen, Tobias G Köllner, Guanglin Li, Guo Wei, Xinlu Chen, Dali Zeng, Qian Qian, Feng Chen
Hao Chen
University of Tennessee CITY: Knoxville United States Of America [US]
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: 8510994@qq.com
Tobias G Köllner
Max Planck Institute for Chemical Ecology CITY: Jena Germany [DE]
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: koellner@ice.mpg.de
Guanglin Li
University of Tennessee CITY: Knoxville United States Of America [US]
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: glli@snnu.edu.cn
Guo Wei
University of Tennessee CITY: Knoxville STATE: Tennessee United States Of America [US]
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: gwei@utk.edu
Xinlu Chen
University of Tennessee CITY: Knoxville STATE: TN United States Of America [US]
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: xchen24@utk.edu
Dali Zeng
China National Rice Research Institute CITY: Hangzhou China [CN]
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: dalizeng@126.com
Qian Qian
State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences CITY: Hangzhou China [CN]
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: qianqian188@hotmail.com
Feng Chen
University of Tennessee CITY: Knoxville STATE: Tennessee POSTAL_CODE: 37996 United States Of America [US]
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: fengc@utk.edu

Published November 2019. DOI: https://doi.org/10.1104/pp.19.00948

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading
  • {copyright, serif} 2019 American Society of Plant Biologists. All rights reserved.

Abstract

Terpenes are specialized metabolites ubiquitously produced by plants via the action of terpene synthases (TPSs). There are enormous variations in the types and amounts of terpenes produced by individual species. To understand the mechanisms responsible for such vast diversity, here we investigated the origin and evolution of a cluster of tandemly arrayed TPS genes in Oryza. In the Oryza species analyzed, TPS genes occur as a three-TPS cluster, a two-TPS cluster, and a single TPS gene in five, one, and one species, respectively. Phylogenetic analysis inferred the origins of the two-TPS and three-TPS clusters and the role of species-specific losses of TPS genes. Within the three-TPS clusters, one orthologous group exhibited conserved catalytic activities. The other two groups, both of which contained pseudogenes and/or nonfunctional genes, exhibited distinct profiles of terpene products. Sequence and structural analysis combined with functional validation identified several amino acids in the active site that are critical for catalytic activity divergence of the three orthologous groups. In the five Oryza species containing the three-TPS cluster, their functional TPS genes showed both conserved and species-specific expression patterns in insect-damaged and untreated plants. Emission patterns of volatile terpenes from each species were largely consistent with the expression of their respective TPS genes and the catalytic activities of the encoded enzymes. This study indicates the importance of combinatorial evolution of TPS genes in determining terpene variations among individual species, which includes gene duplication, retention/loss/degradation of duplicated genes, varying selection pressure, retention/divergence in catalytic activities, and divergence in expression regulation.

  • Received August 1, 2019.
  • Accepted October 28, 2019.

PreviousNext
Back to top
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Plant Physiology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Combinatorial Evolution of a Terpene Synthase Gene Cluster Explains Terpene Variations in Oryza
(Your Name) has sent you a message from Plant Physiology
(Your Name) thought you would like to see the Plant Physiology web site.
Citation Tools
Origin and evolution of tandemly arrayed TPS genes
Hao Chen, Tobias G Köllner, Guanglin Li, Guo Wei, Xinlu Chen, Dali Zeng, Qian Qian, Feng Chen
Plant Physiology Nov 2019, pp.00948.2019; DOI: 10.1104/pp.19.00948

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Request Permissions
Share
Origin and evolution of tandemly arrayed TPS genes
Hao Chen, Tobias G Köllner, Guanglin Li, Guo Wei, Xinlu Chen, Dali Zeng, Qian Qian, Feng Chen
Plant Physiology Nov 2019, pp.00948.2019; DOI: 10.1104/pp.19.00948
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF

In this issue

Plant Physiology: 181 (4)
Plant Physiology
Vol. 181, Issue 4
Dec 2019
  • Table of Contents
  • Table of Contents (PDF)
  • Cover (PDF)
  • About the Cover
  • Index by author

Similar Articles

Our Content

  • Home
  • Current Issue
  • Plant Physiology Preview
  • Archive
  • Focus Collections
  • Classic Collections
  • The Plant Cell
  • Plant Direct
  • Plantae
  • ASPB

For Authors

  • Instructions
  • Submit a Manuscript
  • Editorial Board and Staff
  • Policies
  • Recognizing our Authors

For Reviewers

  • Instructions
  • Journal Miles
  • Policies

Other Services

  • Permissions
  • Librarian resources
  • Advertise in our journals
  • Alerts
  • RSS Feeds

Copyright © 2019 by The American Society of Plant Biologists

Powered by HighWire