About the Cover
On the Cover: Yeast provides a powerful genetic system in which to study gene function. In the event that a null mutation does not produce an obvious phenotype, a synthetic enhancement screen can be performed to identify genetic interaction with the gene being studied and generate an informative phenotype. Synthetic enhancement mutants will maintain a plasmid containing the gene of interest even on non-selective medial. An ADE3 gene allows visual screening for the presence of the plasmid, providing the basis for the sectoring assay. Brands and Ho (pp. 1121-1131) show that mutations in SEY1, a homolog of the Arabidopsis RHD3 gene, synthetically enhance a deletion of YOP1, the yeast homolog of the barley ABA-inducible gene, HVA22, leading to a secretion defect. The cover shows a yop1Ä/sey1 double mutant that has been transformed with a second YOP1-containing plasmid, relieving the requirement for the YOP1 ADE3 plasmid, allowing the formation of sectored colonies.
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Copyright © 2007 by the American Society of Plant Biologists
