About the Cover
On the Cover: Confocal three-dimensional maximum intensity projection of a pea (Pisum sativum) leaf cross section showing chlorophyll autofluorescence (red color) and nitric oxide (NO) production detected by 4,5-diaminofluorescein diacetate (green color) from pea plants treated with 50 µM CdCl2 and 10 mM Ca(NO3)2. Long-term exposure of pea plants to Cd induces a reduction of NO production in pea leaves; however, this fact could be reversed by supplying Ca to the nutrient solution. This result suggests that Cd can affect NO accumulation by promoting Ca deficiency, which can be responsible for the reduction of the NO synthase-like activity. In mesophyll cells the main production of NO took place in small spherical organelles, which may be peroxisomes or mitochondria, although the highest production could be observed associated to tracheids. Confocal image by María Rodríguez-Serrano. (See Rodríguez-Serrano et al., pp. 229–243.)
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Copyright © 2009 by the American Society of Plant Biologists
