PT - JOURNAL ARTICLE AU - Perez-Prat, Eva AU - Narasimhan, Meena L. AU - Binzel, Marla L. AU - Botella, Miguel A. AU - Chen, Zutang AU - Valpuesta, Victoriano AU - Bressan, Ray A. AU - Hasegawa, Paul M. TI - Induction of a Putative Ca<sup>2+</sup>-ATPase mRNA in NaCl-Adapted Cells AID - 10.1104/pp.100.3.1471 DP - 1992 Nov 01 TA - Plant Physiology PG - 1471--1478 VI - 100 IP - 3 4099 - http://www.plantphysiol.org/content/100/3/1471.short 4100 - http://www.plantphysiol.org/content/100/3/1471.full SO - Plant Physiol.1992 Nov 01; 100 AB - A cDNA clone was isolated that encodes the partial sequence of a putative endoplasmic reticulum Ca2+-ATPase of tobacco. The 1.497-kb insert had an open reading frame of 1.149 kb. The deduced peptide had the greatest homology to the endoplasmic reticulum Ca2+-ATPases of Drosophila and Artemia, followed by the mammalian and avian enzymes (SERCA2 and 3). The cDNA insert hybridized to a single mRNA of 4.4 kb from tobacco cultured cells or plant tissues. The level of this transcript was induced about 2-fold by NaCl shock in 428 mm NaCl-deadapted tobacco cells that were maintained in medium without salt, but not in unadapted cells. The level of this transcript was 3- to 4-fold higher in 428 mm NaCl-adapted cells growing in salt than in unadapted cells growing without salt.