PT  - JOURNAL ARTICLE
AU  - Reynolds, Thomas L.
AU  - Kitto, Sherry L.
TI  - Identification of Embryoid-Abundant Genes That Are Temporally Expressed during Pollen Embryogenesis in Wheat Anther Cultures
AID  - 10.1104/pp.100.4.1744
DP  - 1992 Dec 01
TA  - Plant Physiology
PG  - 1744--1750
VI  - 100
IP  - 4
4099  - http://www.plantphysiol.org/content/100/4/1744.short
4100  - http://www.plantphysiol.org/content/100/4/1744.full
SO  - Plant Physiol.1992 Dec 01; 100
AB  - Uninucleate microspores in anther cultures of bread wheat (Triticum aestivum cv Pavon) are capable of producing haploid pollen embryoids and plants. To gain an understanding of this alternate pathway of pollen development, we constructed a cDNA library to young pollen embryoids, isolated embryoid-specific genes, and analyzed their expression patterns during morphogenesis. Two embryoid-abundant clones, pEMB4 and 94, were expressed very early during culture, suggesting that these genes are associated with development and are not simply expressed as a consequence of differentiation. The accumulation patterns of five cloned mRNAs may indicate the activation of specific genes associated with the major morphological and physiological activities connected with the differentiation of embryoids in vitro. These results suggest that embryoid-abundant gene expression is causally related to this pathway because gene expression is spatially and temporally specific and is not observed when microspores are cultured under noninductive conditions.