PT  - JOURNAL ARTICLE
AU  - Bach, M.
AU  - Schnitzler, J. P.
AU  - Seitz, H. U.
TI  - Elicitor-Induced Changes in Ca2+ Influx, K+ Efflux, and 4-Hydroxybenzoic Acid Synthesis in Protoplasts of Daucus carota L
AID  - 10.1104/pp.103.2.407
DP  - 1993 Oct 01
TA  - Plant Physiology
PG  - 407--412
VI  - 103
IP  - 2
4099  - http://www.plantphysiol.org/content/103/2/407.short
4100  - http://www.plantphysiol.org/content/103/2/407.full
SO  - Plant Physiol.1993 Oct 01; 103
AB  - Suspension-cultured carrot cells (Daucus carota) and their protoplasts respond to a fungal elicitor prepared from the culture medium of Pythium aphanidermatum by accumulating 4-hydroxybenzoic acid (4-HBA). Protoplasts release the compound into the culture medium. Using 45CaCl2 as a tracer, we were able to demonstrate that the secretion of 4-HBA is preceded by a rapid increase in the Ca2+ influx and a concomitant K+ efflux. If the increased Ca2+ influx was prevented by ethyleneglycol-bis([beta]-aminoethylether)-N,N[prime]-tetraacetic acid, 4-HBA synthesis was inhibited by 70%. These results are discussed with regard to signal transduction from the plasma membrane to the nucleus of carrot protoplasts.