RT Journal Article
SR Electronic
T1 Elicitor-Induced Changes in Ca2+ Influx, K+ Efflux, and 4-Hydroxybenzoic Acid Synthesis in Protoplasts of Daucus carota L
JF Plant Physiology
JO Plant Physiol.
FD American Society of Plant Biologists
SP 407
OP 412
DO 10.1104/pp.103.2.407
VO 103
IS 2
A1 Bach, M.
A1 Schnitzler, J. P.
A1 Seitz, H. U.
YR 1993
UL http://www.plantphysiol.org/content/103/2/407.abstract
AB Suspension-cultured carrot cells (Daucus carota) and their protoplasts respond to a fungal elicitor prepared from the culture medium of Pythium aphanidermatum by accumulating 4-hydroxybenzoic acid (4-HBA). Protoplasts release the compound into the culture medium. Using 45CaCl2 as a tracer, we were able to demonstrate that the secretion of 4-HBA is preceded by a rapid increase in the Ca2+ influx and a concomitant K+ efflux. If the increased Ca2+ influx was prevented by ethyleneglycol-bis([beta]-aminoethylether)-N,N[prime]-tetraacetic acid, 4-HBA synthesis was inhibited by 70%. These results are discussed with regard to signal transduction from the plasma membrane to the nucleus of carrot protoplasts.