PT  - JOURNAL ARTICLE
AU  - Flury, T.
AU  - Wagner, E.
AU  - Kreuz, K.
TI  - An Inducible Glutathione S-Transferase in Soybean Hypocotyl Is Localized in the Apoplast
AID  - 10.1104/pp.112.3.1185
DP  - 1996 Nov 01
TA  - Plant Physiology
PG  - 1185--1190
VI  - 112
IP  - 3
4099  - http://www.plantphysiol.org/content/112/3/1185.short
4100  - http://www.plantphysiol.org/content/112/3/1185.full
SO  - Plant Physiol.1996 Nov 01; 112
AB  - Glutathione S-transferases (GSTs) with additional activities as fatty acid hydroperoxidases were investigated in soybean (Glycine max L.) hypocotyls. Aside from the GSTs present in total soluble tissue extracts, enzyme activities and distinct immunoreactive GST polypeptides were also detected in the intercellular washing fluid. Whereas the intracellular isoenzymes were both constitutive and inducible, apoplastic GST and glutathione peroxidase was detectable only in tissues treated with the known GST inducer 2,3,5-triiodobenzoic acid. Monensin inhibited the induced accumulation of apoplastic GST but did not affect the intracellular isoforms. The discovery of apoplastic inducible GST will be discussed in light of the putative function of these enzymes in plants.