RT Journal Article SR Electronic T1 Gibberellin Dose-Response Regulation of GA4 Gene Transcript Levels in Arabidopsis JF Plant Physiology JO Plant Physiol. FD American Society of Plant Biologists SP 1195 OP 1203 DO 10.1104/pp.117.4.1195 VO 117 IS 4 A1 Cowling, Rachel J. A1 Kamiya, Yuji A1 Seto, Hideharu A1 Harberd, Nicholas P. YR 1998 UL http://www.plantphysiol.org/content/117/4/1195.abstract AB The gibberellins (GAs) are a complex family of diterpenoid compounds, some of which are potent endogenous regulators of plant growth. As part of a feedback control of endogenous GA levels, active GAs negatively regulate the abundance of mRNA transcripts encoding GA biosynthesis enzymes. For example, Arabidopsis GA4 gene transcripts encode GA 3β-hydroxylase, an enzyme that catalyzes the conversion of inactive to active GAs. Here we show that active GAs regulateGA4 transcript abundance in a dose-dependent manner, and that down-regulation of GA4 transcript abundance is effected by GA4 (the product of 3β-hydroxylation) but not by its immediate precursor GA9 (the substrate). Comparison of several different GA structures showed that GAs active in promoting hypocotyl elongation were also active in regulating GA4transcript abundance, suggesting that similar GA:receptor and subsequent signal transduction processes control these two responses. It is interesting that these activities were not restricted to 3β-hydroxylated GAs, being also exhibited by structures that were not 3β-hydroxylated but that had another electronegative group at C-3. We also show that GA-mediated control of GA4 transcript abundance is disrupted in the GA-response mutants gaiand spy-5. These observations define a sensitive homeostatic mechanism whereby plants may regulate their endogenous GA levels. BX-112prohexadione calcium BX-112PACpaclobutrazolQRT-PCRquantitative RT-PCRRT-PCRreverse-transcription PCR