RT Journal Article SR Electronic T1 Characterization of N-Glycans from Arabidopsis. Application to a Fucose-Deficient Mutant JF Plant Physiology JO Plant Physiol. FD American Society of Plant Biologists SP 725 OP 734 DO 10.1104/pp.119.2.725 VO 119 IS 2 A1 Rayon, Catherine A1 Cabanes-Macheteau, Marion A1 Loutelier-Bourhis, Corinne A1 Salliot-Maire, Isabelle A1 Lemoine, Jérome A1 Reiter, Wolf-Dieter A1 Lerouge, Patrice A1 Faye, Loı̈c YR 1999 UL http://www.plantphysiol.org/content/119/2/725.abstract AB The structures of glycansN-linked to Arabidopsis proteins have been fully identified. From immuno- and affinodetections on blots, chromatography, nuclear magnetic resonance, and glycosidase sequencing data, we show that Arabidopsis proteins areN-glycosylated by high-mannose-typeN-glycans from Man5GlcNAc2 to Man9GlcNAc2, and by xylose- and fucose (Fuc)-containing oligosaccharides. However, complex biantenary structures containing the terminal Lewis a epitope recently reported in the literature (A.-C. Fitchette-Lainé, V. Gomord, M. Cabanes, J.-C. Michalski, M. Saint Macary, B. Foucher, B. Cavalier, C. Hawes, P. Lerouge, and L. Faye [1997] Plant J 12: 1411–1417) were not detected. A similar study was done on the Arabidopsismur1 mutant, which is affected in the biosynthesis ofl-Fuc. In this mutant, one-third of the Fuc residues of the xyloglucan has been reported to be replaced by l-galactose (Gal) (E. Zablackis, W.S. York, M. Pauly, S. Hantus, W.D. Reiter, C.C.S. Chapple, P. Albersheim, and A. Darvill [1996] Science 272: 1808–1810). N-linked glycans from the mutant were identified and their structures were compared with those isolated from the wild-type plants. In about 95% of all N-linked glycans from the mur1 plant, l-Fuc residues were absent and were not replaced by another monosaccharide. However, in the remaining 5%, l-Fuc was found to be replaced by a hexose residue. From nuclear magnetic resonance and mass spectrometry data of the mur1 N-glycans, and by analogy with data reported on mur1 xyloglucan, this subpopulation ofN-linked glycans was proposed to bel-Gal-containing N-glycans resulting from the replacement of l-Fuc by l-Gal. CID-MS-MScollision-induced dissociation MSHPAEChigh pH anion-exchange chromatographyHPAEC-PADHPAEC coupled to pulsed amperometric detectionPApyridylamino derivativePNGase Apeptide N-glycosidase A