PT  - JOURNAL ARTICLE
AU  - Piffanelli, Pietro
AU  - Zhou, Fasong
AU  - Casais, Catarina
AU  - Orme, James
AU  - Jarosch, Birgit
AU  - Schaffrath, Ulrich
AU  - Collins, Nicholas C.
AU  - Panstruga, Ralph
AU  - Schulze-Lefert, Paul
TI  - The Barley MLO Modulator of Defense and Cell Death Is Responsive to Biotic and Abiotic Stress Stimuli
AID  - 10.1104/pp.010954
DP  - 2002 Jul 01
TA  - Plant Physiology
PG  - 1076--1085
VI  - 129
IP  - 3
4099  - http://www.plantphysiol.org/content/129/3/1076.short
4100  - http://www.plantphysiol.org/content/129/3/1076.full
SO  - Plant Physiol.2002 Jul 01; 129
AB  - Lack of the barley (Hordeum vulgare) seven-transmembrane domain MLO protein confers resistance against the fungal pathogen Blumeria graminis f. sp.hordei (Bgh). To broaden the basis for MLO structure/function studies, we sequenced additionalmlo resistance alleles, two of which confer only partial resistance. Wild-type MLO dampens the cell wall-restricted hydrogen peroxide burst at points of attempted fungal penetration of the epidermal cell wall, and in subtending mesophyll cells, it suppresses a second oxidative burst and cell death. Although theBgh-induced cell death in mlo plants is spatially and temporally separated from resistance, we show that the two processes are linked. Uninoculated mutant mlo plants exhibit spontaneous mesophyll cell death that appears to be part of accelerated leaf senescence. Mlo transcript abundance increases in response to Bgh, rice (Oryza sativa) blast, wounding, paraquat treatment, a wheat powdery mildew-derived carbohydrate elicitor, and during leaf senescence. This suggests a broad involvement of Mlo in cell death protection and in responses to biotic and abiotic stresses.