RT Journal Article
SR Electronic
T1 The Barley MLO Modulator of Defense and Cell Death Is Responsive to Biotic and Abiotic Stress Stimuli
JF Plant Physiology
JO Plant Physiol.
FD American Society of Plant Biologists
SP 1076
OP 1085
DO 10.1104/pp.010954
VO 129
IS 3
A1 Piffanelli, Pietro
A1 Zhou, Fasong
A1 Casais, Catarina
A1 Orme, James
A1 Jarosch, Birgit
A1 Schaffrath, Ulrich
A1 Collins, Nicholas C.
A1 Panstruga, Ralph
A1 Schulze-Lefert, Paul
YR 2002
UL http://www.plantphysiol.org/content/129/3/1076.abstract
AB Lack of the barley (Hordeum vulgare) seven-transmembrane domain MLO protein confers resistance against the fungal pathogen Blumeria graminis f. sp.hordei (Bgh). To broaden the basis for MLO structure/function studies, we sequenced additionalmlo resistance alleles, two of which confer only partial resistance. Wild-type MLO dampens the cell wall-restricted hydrogen peroxide burst at points of attempted fungal penetration of the epidermal cell wall, and in subtending mesophyll cells, it suppresses a second oxidative burst and cell death. Although theBgh-induced cell death in mlo plants is spatially and temporally separated from resistance, we show that the two processes are linked. Uninoculated mutant mlo plants exhibit spontaneous mesophyll cell death that appears to be part of accelerated leaf senescence. Mlo transcript abundance increases in response to Bgh, rice (Oryza sativa) blast, wounding, paraquat treatment, a wheat powdery mildew-derived carbohydrate elicitor, and during leaf senescence. This suggests a broad involvement of Mlo in cell death protection and in responses to biotic and abiotic stresses.