PT  - JOURNAL ARTICLE
AU  - Mroczka, Andrew
AU  - Roberts, Peter D.
AU  - Fillatti, JoAnne J.
AU  - Wiggins, B. Elizabeth
AU  - Ulmasov, Tim
AU  - Voelker, Toni
TI  - An Intron Sense Suppression Construct Targeting Soybean <em>FAD2-1</em> Requires a Double-Stranded RNA-Producing Inverted Repeat T-DNA Insert
AID  - 10.1104/pp.110.154351
DP  - 2010 Jun 01
TA  - Plant Physiology
PG  - 882--891
VI  - 153
IP  - 2
4099  - http://www.plantphysiol.org/content/153/2/882.short
4100  - http://www.plantphysiol.org/content/153/2/882.full
SO  - Plant Physiol.2010 Jun 01; 153
AB  - We demonstrate that the transformation of soybean (Glycine max) with sense suppression constructs using intron sequences from the fatty acid oleyl Δ12 desaturase gene FAD2-1A leads to efficient and specific reduction of FAD2-1 transcripts in developing seeds, increased oleic acid, and decreased polyunsaturated fatty acids. The related FAD2-2 transcripts are only marginally affected. Despite screening a large number of independent transformants, no single-copy efficacious transformants could be found. Invariably, all the least complex transgenic loci have two T-DNA copies in an inverted repeat configuration, centered at the right borders. We show that this T-DNA configuration produces an inverted repeat transcript and that small interfering RNAs accumulate against the target sequence.