RT Journal Article
SR Electronic
T1 An Intron Sense Suppression Construct Targeting Soybean <em>FAD2-1</em> Requires a Double-Stranded RNA-Producing Inverted Repeat T-DNA Insert
JF Plant Physiology
JO Plant Physiol.
FD American Society of Plant Biologists
SP 882
OP 891
DO 10.1104/pp.110.154351
VO 153
IS 2
A1 Mroczka, Andrew
A1 Roberts, Peter D.
A1 Fillatti, JoAnne J.
A1 Wiggins, B. Elizabeth
A1 Ulmasov, Tim
A1 Voelker, Toni
YR 2010
UL http://www.plantphysiol.org/content/153/2/882.abstract
AB We demonstrate that the transformation of soybean (Glycine max) with sense suppression constructs using intron sequences from the fatty acid oleyl Δ12 desaturase gene FAD2-1A leads to efficient and specific reduction of FAD2-1 transcripts in developing seeds, increased oleic acid, and decreased polyunsaturated fatty acids. The related FAD2-2 transcripts are only marginally affected. Despite screening a large number of independent transformants, no single-copy efficacious transformants could be found. Invariably, all the least complex transgenic loci have two T-DNA copies in an inverted repeat configuration, centered at the right borders. We show that this T-DNA configuration produces an inverted repeat transcript and that small interfering RNAs accumulate against the target sequence.