RT Journal Article
SR Electronic
T1 Phenotypical Temperature Adaptation of Protein Synthesis in Wheat Seedlings
JF Plant Physiology
JO Plant Physiol.
FD American Society of Plant Biologists
SP 144
OP 149
DO 10.1104/pp.64.1.144
VO 64
IS 1
A1 Weidner, Manfred
A1 Combrink, Gabriele
YR 1979
UL http://www.plantphysiol.org/content/64/1/144.abstract
AB Optimum temperature and temperature coefficient of protein synthesis in young wheat plants exhibit phenotypical temperature adaptation. In plants grown for 2 days at either chilling (4 C), medium (20 C), or high (36 C) temperature the respective values are: 27 C and 14.2 kilocalories per mole, 31 C and 18.2 kilocalories per mole, 35 C and 23.6 kilocalories per mole, based on in vivo [14C]leucine incorporation into total protein. The validity of the [14C]leucine incubation method has been confirmed by double-labeling experiments. Readaptation time curves are complex: the optimum temperature parameter readjusts within approximately 4 hours to an altered temperature regime, whereas the temperature coefficient needs between 4 and 96 hours for complete readaptation—depending on the temperature conditions prior to the temperature shift. Heat-preadapted plants need a recovery period at medium temperature to regain their cold adaptability with respect to optimum temperature. Cycloheximide (30 micrograms per milliliter) reduces [14C]leucine incorporation into protein by 85%, thus indicating that predominantly the cytoplasmic 80S system of protein synthesis is involved in temperature adaptation.