RT Journal Article
SR Electronic
T1 Investigations of Vacuoles Isolated from Tobacco
JF Plant Physiology
JO Plant Physiol.
FD American Society of Plant Biologists
SP 74
OP 78
DO 10.1104/pp.64.1.74
VO 64
IS 1
A1 Saunders, James A.
YR 1979
UL http://www.plantphysiol.org/content/64/1/74.abstract
AB Nicotine was shown to be associated with mature vacuoles isolated from protoplasts of Nicotiana rustica. The vacuolar preparations also contained high levels of acid phosphatase, ATPase, and approximately 30% of the soluble protoplastic protein. The contamination of the vacuolar isolate by chlorophyll, succinate dehydrogenase, and NADPH cytochrome c reductase (markers for chloroplasts, mitochondria, and endoplasmic reticulum) was low. The enzymic activity associated with the vacuoles was not due to the exogenously supplied digestive enzymes used in the preparation of the protoplast. The relatively easy isolation of tobacco vacuoles makes this an excellent system for biochemical investigations of the vacuole.