PT  - JOURNAL ARTICLE
AU  - Hirel, Bertrand
AU  - Perrot-Rechenmann, Catherine
AU  - Suzuki, Akira
AU  - Vidal, Jean
AU  - Gadal, Pierre
TI  - Glutamine Synthetase in Spinach Leaves
AID  - 10.1104/pp.69.4.983
DP  - 1982 Apr 01
TA  - Plant Physiology
PG  - 983--987
VI  - 69
IP  - 4
4099  - http://www.plantphysiol.org/content/69/4/983.short
4100  - http://www.plantphysiol.org/content/69/4/983.full
SO  - Plant Physiol.1982 Apr 01; 69
AB  - By polyacrylamide gel electrophoresis, DEAE Sephacel, and hydroxyapatite chromatography, one form of glutamine synthetase has been identified in spinach (Spinacia oleracea L. cv. Monstrueux de Viroflay) leaves. It is localized only inside the chloroplast. The enzyme was purified to homogeneity and specific antibodies against the protein were raised by immunization of rabbits. The intracellular localization of glutamine synthetase in spinach leaves was studied by indirect immunofluorescence microscopy on thin-sectioned spinach leaves. It has been demonstrated that the enzyme is specifically associated with the chloroplasts of parenchymatous cells.