RT Journal Article
SR Electronic
T1 Glutamine Synthetase in Spinach Leaves
JF Plant Physiology
JO Plant Physiol.
FD American Society of Plant Biologists
SP 983
OP 987
DO 10.1104/pp.69.4.983
VO 69
IS 4
A1 Hirel, Bertrand
A1 Perrot-Rechenmann, Catherine
A1 Suzuki, Akira
A1 Vidal, Jean
A1 Gadal, Pierre
YR 1982
UL http://www.plantphysiol.org/content/69/4/983.abstract
AB By polyacrylamide gel electrophoresis, DEAE Sephacel, and hydroxyapatite chromatography, one form of glutamine synthetase has been identified in spinach (Spinacia oleracea L. cv. Monstrueux de Viroflay) leaves. It is localized only inside the chloroplast. The enzyme was purified to homogeneity and specific antibodies against the protein were raised by immunization of rabbits. The intracellular localization of glutamine synthetase in spinach leaves was studied by indirect immunofluorescence microscopy on thin-sectioned spinach leaves. It has been demonstrated that the enzyme is specifically associated with the chloroplasts of parenchymatous cells.