RT Journal Article
SR Electronic
T1 Thermolysin Is a Suitable Protease for Probing the Surface of Intact Pea Chloroplasts
JF Plant Physiology
JO Plant Physiol.
FD American Society of Plant Biologists
SP 675
OP 678
DO 10.1104/pp.75.3.675
VO 75
IS 3
A1 Cline, Kenneth
A1 Werner-Washburne, Margaret
A1 Andrews, Jaen
A1 Keegstra, Kenneth
YR 1984
UL http://www.plantphysiol.org/content/75/3/675.abstract
AB Several proteases, i.e., pronase, a mixture of trypsin and chymotrypsin, and thermolysin were screened as potential surface probes of isolated intact pea (Pisum sativum var Laxton's Progress No. 9) chloroplasts. Of these, only thermolysin met the criteria of a suitable probe. Thermolysin destroyed outer envelope polypeptides, but did not affect inner envelope polypeptides, envelope permeability properties or such chloroplast activities as metabolite transport and O2 evolution.