RT Journal Article
SR Electronic
T1 A Rapid and Simple Procedure for Purification of Indole-3-Acetic Acid Prior to GC-SIM-MS Analysis
JF Plant Physiology
JO Plant Physiol.
FD American Society of Plant Biologists
SP 822
OP 825
DO 10.1104/pp.86.3.822
VO 86
IS 3
A1 Chen, Kai-Hsien
A1 Miller, Anita N.
A1 Patterson, Glenn W.
A1 Cohen, Jerry D.
YR 1988
UL http://www.plantphysiol.org/content/86/3/822.abstract
AB A simple and rapid procedure for the purification of indole-3-acetic acid prior to gas chromatography-selected ion monitoring-mass spectrographic analysis was developed using an amino anion exchange minicolumn and a short high resolution C18 column. Since multiple samples can be prepared at one time, the procedure is more rapid and the sample preparation time is reduced to one-third that normally required. In addition, the final recovery was improved by 40 to 50% over that of a solvent partitioning procedure.