PT  - JOURNAL ARTICLE
AU  - Coté, Gary G.
AU  - DePass, Anthony L.
AU  - Quarmby, Lynne M.
AU  - Tate, Bonnie F.
AU  - Morse, M. J.
AU  - Satter, Ruth L.
AU  - Crain, Richard C.
TI  - Separation and Characterization of Inositol Phospholipids from the Pulvini of <em>Samanea saman</em>
AID  - 10.1104/pp.90.4.1422
DP  - 1989 Aug 01
TA  - Plant Physiology
PG  - 1422--1428
VI  - 90
IP  - 4
4099  - http://www.plantphysiol.org/content/90/4/1422.short
4100  - http://www.plantphysiol.org/content/90/4/1422.full
SO  - Plant Physiol.1989 Aug 01; 90
AB  - To supplement current thin-layer chromatographic methods for separation and quantitation of plant phospholipids, an alternative method, high-performance liquid chromatography was developed. The major inositol-containing lipids from the pulvini of Samanea saman Merr. were identified as phosphatidylinositol, phosphatidylinositol phosphate, and phosphatidylinositol bisphosphate based on comigration with authentic standards on high-performance liquid chromatography and on thin-layer chromatography. The patterns of incorporation of radioactivity into the putative phosphatidylinositol and phosphatidylinositol phosphate were consistent with these identifications when pulvini were labeled with [3H]glycerol, [3H]inositol, or [32P]orthophosphate. Analysis of the products of enzymic hydrolysis, of chemical deacylation, and of `fingerprint' methanolysis of these phospholipids confirmed the identifications.