RT Journal Article
SR Electronic
T1 Separation and Characterization of Inositol Phospholipids from the Pulvini of <em>Samanea saman</em>
JF Plant Physiology
JO Plant Physiol.
FD American Society of Plant Biologists
SP 1422
OP 1428
DO 10.1104/pp.90.4.1422
VO 90
IS 4
A1 Coté, Gary G.
A1 DePass, Anthony L.
A1 Quarmby, Lynne M.
A1 Tate, Bonnie F.
A1 Morse, M. J.
A1 Satter, Ruth L.
A1 Crain, Richard C.
YR 1989
UL http://www.plantphysiol.org/content/90/4/1422.abstract
AB To supplement current thin-layer chromatographic methods for separation and quantitation of plant phospholipids, an alternative method, high-performance liquid chromatography was developed. The major inositol-containing lipids from the pulvini of Samanea saman Merr. were identified as phosphatidylinositol, phosphatidylinositol phosphate, and phosphatidylinositol bisphosphate based on comigration with authentic standards on high-performance liquid chromatography and on thin-layer chromatography. The patterns of incorporation of radioactivity into the putative phosphatidylinositol and phosphatidylinositol phosphate were consistent with these identifications when pulvini were labeled with [3H]glycerol, [3H]inositol, or [32P]orthophosphate. Analysis of the products of enzymic hydrolysis, of chemical deacylation, and of `fingerprint' methanolysis of these phospholipids confirmed the identifications.