PT  - JOURNAL ARTICLE
AU  - Dussert, Stéphane
AU  - Guerin, Chloé
AU  - Andersson, Mariette
AU  - Joët, Thierry
AU  - Tranbarger, Timothy J.
AU  - Pizot, Maxime
AU  - Sarah, Gautier
AU  - Omore, Alphonse
AU  - Durand-Gasselin, Tristan
AU  - Morcillo, Fabienne
TI  - Comparative Transcriptome Analysis of Three Oil Palm Fruit and Seed Tissues That Differ in Oil Content and Fatty Acid Composition
AID  - 10.1104/pp.113.220525
DP  - 2013 Jul 01
TA  - Plant Physiology
PG  - 1337--1358
VI  - 162
IP  - 3
4099  - http://www.plantphysiol.org/content/162/3/1337.short
4100  - http://www.plantphysiol.org/content/162/3/1337.full
SO  - Plant Physiol.2013 Jul 01; 162
AB  - Oil palm (Elaeis guineensis) produces two oils of major economic importance, commonly referred to as palm oil and palm kernel oil, extracted from the mesocarp and the endosperm, respectively. While lauric acid predominates in endosperm oil, the major fatty acids (FAs) of mesocarp oil are palmitic and oleic acids. The oil palm embryo also stores oil, which contains a significant proportion of linoleic acid. In addition, the three tissues display high variation for oil content at maturity. To gain insight into the mechanisms that govern such differences in oil content and FA composition, tissue transcriptome and lipid composition were compared during development. The contribution of the cytosolic and plastidial glycolytic routes differed markedly between the mesocarp and seed tissues, but transcriptional patterns of genes involved in the conversion of sucrose to pyruvate were not related to variations for oil content. Accumulation of lauric acid relied on the dramatic up-regulation of a specialized acyl-acyl carrier protein thioesterase paralog and the concerted recruitment of specific isoforms of triacylglycerol assembly enzymes. Three paralogs of the WRINKLED1 (WRI1) transcription factor were identified, of which EgWRI1-1 and EgWRI1-2 were massively transcribed during oil deposition in the mesocarp and the endosperm, respectively. None of the three WRI1 paralogs were detected in the embryo. The transcription level of FA synthesis genes correlated with the amount of WRI1 transcripts and oil content. Changes in triacylglycerol content and FA composition of Nicotiana benthamiana leaves infiltrated with various combinations of WRI1 and FatB paralogs from oil palm validated functions inferred from transcriptome analysis.GlossaryFAfatty acidPUFApolyunsaturated fatty acidACPacyl carrier proteinHCAHierarchical Clustering AnalysisDECdifferentially expressed contigERendoplasmic reticulumACAudic-ClaverieFDRFalse Discovery RateTAGtriacylglycerolMCFAmedium-chain fatty acidDAPd after pollinationPDHpyruvate dehydrogenaseLACSlong-chain acyl-CoA synthesisSADstearate desaturasePDATphospholipid:diacylglycerol acyltransferasePDCTphosphatidylcholine:diacylglycerol cholinephosphotransferaseGPATglycerol-3-P-acyltransferaseLPCAT1-acylglycerol-3-phosphocholine acyltransferaseENOenolaseGAPDHglyceraldehyde 3-P dehydrogenasePEPphosphoenolpyruvatePPiinorganic pyrophosphatePPiaseinorganic pyrophosphatasePFPPPi-dependent phosphofructokinaseTPTtriose-P/phosphate translocatorPGKphosphoglycerate kinasePGLYMphosphoglycerate mutaseCPTcholinephosphotransferase1.3PGA1,3-bisphosphoglycerateFAMEfatty acid methyl estersPA1,2-diacylglycerol-3-PPCphosphatidylcholinePFGATP-dependent phosphofructokinase