Table I. FA composition of transgenic Arabidopsis seeds of T4 lines expressing castor RcFAH12 and RcROD1

The transgenic lines of these genes were obtained from independent transformation events. Data represent measurements of three homozygous lines (means ± se).

LineFA CompositionTotal HFAa
16:018:018:118:218:320:118:1OH18:2OH20:1OH
wt % of total
Col-08.0 ± 0.12.7 ± 0.115.8 ± 0.230.6 ± 0.218.7 ± 0.121.3 ± 0.1
rod17.9 ± 0.12.5 ± 0.133.5 ± 0.215.5 ± 0.113.1 ± 0.125.3 ± 0.1
rod1-RcROD17.2 ± 0.53.0 ± 0.516.2 ± 1.729.7 ± 1.217.6 ± 0.420.7 ± 0.9
rod1-FAH1212.4 ± 0.65.2 ± 0.238.3 ± 0.814.5 ± 0.35.9 ± 0.115.4 ± 0.52.8 ± 0.11.2 ± 0.11.5 ± 0.15.5 ± 0.1
rod1-FAH12/RcROD110.1 ± 0.14.8 ± 0.225.5 ± 0.324.3 ± 0.211.6 ± 0.511.1 ± 1.56.2 ± 0.23.0 ± 0.40.6 ± 0.19.7 ± 0.6
Col-FAH1210.1 ± 0.44.7 ± 0.319.6 ± 0.422.2 ± 0.210.7 ± 0.917.9 ± 0.26.3 ± 0.12.1 ± 0.11.6 ± 0.19.9 ± 0.3
Col-FAH12/RcROD110.0 ± 0.55.1 ± 0.324.2 ± 0.717.5 ± 1.25.2 ± 0.114.9 ± 0.612.7 ± 0.24.6 ± 0.52.6 ± 0.119.9 ± 0.5
  • a Excluding small amounts of 20:2OH.